Abstract: Objective To investigate the expression levels and clinical significance of serum miR-205 and miR-944 in patients diagnosed with cervical cancer. Methods One hundred sixty two patients with cervical cancer（cervical cancer group）, 85 patients with cervical intraepithelial neoplasia（CIN group）and 65 healthy women（control group）in our hospital were selected from January 2017 to December 2019. The expression levels of serum miR-205 and miR-944 in each group were detected, and the relationship between expression levels and clinicopathological characteristics was analyzed. The diagnostic value of miR-205 and miR-944 in cervical cancer was analyzed using receiver operating characteristic (ROC) curve. Pearson correlation analysis was used to analyze the correlation between miR-205 and miR-944. Results The serum levels of miR-205（1.25±0.61 vs 0.71±0.13, 0.14±0.10）and miR-944（0.82±0.38 vs 0.46±0.02, 0.03±0.01）in cervical cancer group were significantly higher than those in CIN group and control group (P<0.01). In cervical cancer patients, the expression levels of miR-205 and miR-944 were correlated to clinical stage, depth of invasion and lymph node metastasis ( P<0.05). The ROC curves showed that the optimal cut-off values of miR-205 and miR-944 for diagnostic tests of cervical cancer were 0.94 and 0.53, respectively. The area under the curve (AUC) of the two combined diagnosis of cervical cancer was the largest（0.926, 95% CI: 0.864-0.985）, with 92.0% sensitivity, 88.7% specificity. The correlation analysis showed that there was a positive relationship between miR-205 and miR-944 in cervical cancer patients（r=0.824, P<0.01）. Conclusion The expression levels of miR-205 and miR-944 in cervical cancer patients were significantly higher, and a combination of the two tests can help guide the diagnosis of cervical cancer.

Key words: Cervical cancer, MiR-20, MiR-944, Clinical significance