Objective To investigate the level and clinical value of autoantibodies in patients with malignant pulmonary nodules. Methods Seventy-one patients with asymptomatic pulmonary nodules admitted to our hospital from November 2018 to January 2019 were enrolled, including 58 malignant pulmonary nodules and 13 benign pulmonary nodules. And ten healthy people as control group.The levels of p53, PGP9.5, SOX2, GAGE7, GBU4-5, MAGEA1 and CAGE in all specimens were detected by ELISA kit. The ROC curve was used to analyze the diagnostic value of autoantibodies for malignant pulmonary nodules. Results Compared with the benign pulmonary nodules group and the normal control group, the level of autoantibodies in the malignant nodules of the lungs was significantly increased (P<0.05);ROC curve analysis showed that seven autoantibodies were important for the differential diagnosis of benign and malignant pulmonary nodules (AUC>0.5, P<0.05), and seven autoantibodies combined detection could improve the differential diagnosis efficiency (AUC=0.822);The positive detection rate of lung malignant nodules was 81.7% by LDCT and was increased to 94.0% combining with autoantibodies detection (P<0.05). Conclusion Serum autoantibody detection has potentialclinical value in the differential diagnosis of benign and malignant pulmonary nodules.

Objective To compare the consistence of HPV typing tests by using three kits that are domestically available. Methods A total of 82 positive samples and 10 negative samples were collected that had been detected with Liferiver kits in recent 2 months and the Results of HPV typing tests were compared for the agreements with the kits purchased from other two companies of Kaipu and Amply. Results The repeatability of the Results in the three domestic kits were 95.6 %,96.7% and 95.6%,respectively. The consistent rate was 51.0%（47/92）,the partial consistent rate was 23.9%（22/92）,and the inconsistent rate was 25.0%（23/92）.The consistent rate of 86.4%（32/37）was seen in detections of single type infection,22.2%（10/45）in multiple infections,and the partial consistent rate found to be 48.9%（22/45）. Conclusion of the Results in the three domestic kits varied,and the hospital should be fixed for HPV typing before and after treatments of the infection.

Objective The invalid results and listsmay influence thetimely sending ofthe nucleic acid test reports. This work aimed to explore the reason for the invalid results and to optimize the corresponding operations for safety of blood transfusion. Methods Blood samples were collected from the voluntarydonors in Anyang from Jun 2016 to may 2017and DNAs were extracted withHaoyuan ChiTas BSS1200 automatic nucleic acid separator and subsequently amplified with ABI7500 Fluorescent Amplifier. The invalid results and lists were analyzed. Results A total of 88739 samples were tested for DNAs and 0.06% results and 1.04% lists were found to be invalid. Further analyses of the invalid results showed that it is not the batche's number of reagent but the insufficient amounts of samples as well as personal operation errorsor laboratory contaminationsthat causedthe invalid amplification of internal standard. Conclusion The invalid results and lists can be minimized by enhancing the training of the staff who are involved in nucleic acid tests,improving operating procedures,and avoiding laboratory contaminations by daily cleaning and disinfecting.

Objective To retrospectively analyze the influencing factors of arachidonic acid （AA） and adenosine diphosphate （ADP） inhibition rates and the efficacy of different antiplatelet drugs. Methods A total of 114 patients with platelet inhibition rates detected in the hospital were selected from March 2015 to December 2018 . The inhibition rates of AA and ADP were divided into several groups according to the antiplatelet treatment strategies. Results The ADP inhibition rate,rather than the AA inhibition rate（P>0.05）,was negatively correlated with age （P<0.01） and positively with triglyceride （TG） test results （P<0.01）.Significant differences of ADP inhibition rates between groups of aspirin and aspirin+Greg los（P< 0.01） and between groups of clopidogrel and aspirin+Greg los（P<0.05） were noted.Similarly,remarkable differences of AA inhibition rates between groups of aspirin and clopidogrel（P<0.01）,clopidogrel and aspirin+Greg los（P<0.01）,and clopidogrel and aspirin+clopidogrel（P<0.05） were seen. Conclusion A comprehensive analysis of age and TG level should be taken into account in terms of the ADP inhibitory rate detection.Greg los treatment is effective based on the ADP inhibition rate measurement while aspirin therapy is promising based on AA inhibition.

Objective To explore the effective management of blood bag recycling and improve the awareness of the importance of blood bag recycling in blood transfusion departments. Methods The clinical use of blood and blood bag recycling rate were calculated within 24h in the hospital from April to September 2017,the reasons for the low recycling rate of the blood bag were analyzed timely so as to improve the measures and sum up the experience. Results The 24h blood bag recycling rate was 78.54% in April 2017, after appropriate measures were taken,with a greatly improved situation in the later months,and clinical departments paid more attention to the implementation of blood bag recycling work. Conclusion The safety of clinical transfusion needs a participation of all related personels. The PDCA circulation method that includes planing,doing,checking,and action can continuously improve the rate of timely blood bag recycling in the security ensurance of clinical blood transfusion.

Objective To choose a more reliable method to determine the hemoglobin concentration of blood donors in order to further improve the accuracy. Methods On the basis of gold standard method for the detection of hemoglobin (HiCN method), we used blood cell analyzer as the control group. Comparison test was carried out between visual reagent on hemoglobin (copper sulfate proportion) and dry hemoglobin test paper. Results Regression analysis of 151 cases of data samples with confidence degree P =95% was carried out. Comparison of Uritest detection(y) and Sysmex reference(x), y=0.892 2x+15.979, R ²=0.7461; comparison of Hemocue detection(y) and Sysmex reference(x), y=0.949 4x+15.018, R ² =0.838 1. Conclusion Determination of hemoglobin with dry hemoglobin test paper is a method with similar sensitivity and stability with that of the method using blood cell analyzer. The precision, accuracy and linear degree fully meet the needs of testing. It has practical significance in especially blood screening test in blood banks.

Objective To investigate the efficacy of batroxobin and vasodilators in the treatment of sudden deafness and coagulations. Methods Eighty-four patients with sudden deafness admitted to the hospital from 2015 to 2017 were randomLy divided into two groups,with 42 cases in each. The control was treated with vasodilators and the observation group was with batroxobin. Clinical efficacy and changes in coagulation parameters of pre- and post-treatments were compared between the two groups. Results The clinical curative effect of the observation group was higher than that of the control （P<0.05）. The prothrombin time,thrombin time and activated partial thromboplastin time in the observation group were prolonged than those in the control. The levels of fibrinogen and platelet factorswere found to be lowedin the observation group （P<0.05）. Conclusion The efficacy of batroxobin treatment in patients with sudden deafness is superior to that of vasodilators,which can effectively improve the coagulation function of patients and is worthy of clinical application.

ObjectiveTo explore the necessity and feasibility of RBC blood typing by molecular technique for unidentified blood group. MethodsThe blood group was identified by serological test and molecular detection. Results

Objective Identification of a rare blood type of Di(a+b-) with anti-Dib antibody by serological and molecular biology technique. Methods The patient's blood type, direct anti-globulin test, and irregular antibody screening and identification were performed by serology means. DNA sequencing was used to identify the Diego blood type. Results The patient has irregular antibodies against highly prevalent antigens. DNA sequencing confirmed the rare Di(a+b-) blood type. Conclusion Blood typing by DNA sequencing is pivotal for identification of antibodies against highly prevalent antigens.