Objective To compare the consistence of HPV typing tests by using three kits that are domestically available. Methods A total of 82 positive samples and 10 negative samples were collected that had been detected with Liferiver kits in recent 2 months and the Results of HPV typing tests were compared for the agreements with the kits purchased from other two companies of Kaipu and Amply. Results The repeatability of the Results in the three domestic kits were 95.6 %,96.7% and 95.6%,respectively. The consistent rate was 51.0%（47/92）,the partial consistent rate was 23.9%（22/92）,and the inconsistent rate was 25.0%（23/92）.The consistent rate of 86.4%（32/37）was seen in detections of single type infection,22.2%（10/45）in multiple infections,and the partial consistent rate found to be 48.9%（22/45）. Conclusion of the Results in the three domestic kits varied,and the hospital should be fixed for HPV typing before and after treatments of the infection.

Objective To investigate the level and clinical value of autoantibodies in patients with malignant pulmonary nodules. Methods Seventy-one patients with asymptomatic pulmonary nodules admitted to our hospital from November 2018 to January 2019 were enrolled, including 58 malignant pulmonary nodules and 13 benign pulmonary nodules. And ten healthy people as control group.The levels of p53, PGP9.5, SOX2, GAGE7, GBU4-5, MAGEA1 and CAGE in all specimens were detected by ELISA kit. The ROC curve was used to analyze the diagnostic value of autoantibodies for malignant pulmonary nodules. Results Compared with the benign pulmonary nodules group and the normal control group, the level of autoantibodies in the malignant nodules of the lungs was significantly increased (P<0.05);ROC curve analysis showed that seven autoantibodies were important for the differential diagnosis of benign and malignant pulmonary nodules (AUC>0.5, P<0.05), and seven autoantibodies combined detection could improve the differential diagnosis efficiency (AUC=0.822);The positive detection rate of lung malignant nodules was 81.7% by LDCT and was increased to 94.0% combining with autoantibodies detection (P<0.05). Conclusion Serum autoantibody detection has potentialclinical value in the differential diagnosis of benign and malignant pulmonary nodules.

Objective To retrospectively analyze the influencing factors of arachidonic acid （AA） and adenosine diphosphate （ADP） inhibition rates and the efficacy of different antiplatelet drugs. Methods A total of 114 patients with platelet inhibition rates detected in the hospital were selected from March 2015 to December 2018 . The inhibition rates of AA and ADP were divided into several groups according to the antiplatelet treatment strategies. Results The ADP inhibition rate,rather than the AA inhibition rate（P>0.05）,was negatively correlated with age （P<0.01） and positively with triglyceride （TG） test results （P<0.01）.Significant differences of ADP inhibition rates between groups of aspirin and aspirin+Greg los（P< 0.01） and between groups of clopidogrel and aspirin+Greg los（P<0.05） were noted.Similarly,remarkable differences of AA inhibition rates between groups of aspirin and clopidogrel（P<0.01）,clopidogrel and aspirin+Greg los（P<0.01）,and clopidogrel and aspirin+clopidogrel（P<0.05） were seen. Conclusion A comprehensive analysis of age and TG level should be taken into account in terms of the ADP inhibitory rate detection.Greg los treatment is effective based on the ADP inhibition rate measurement while aspirin therapy is promising based on AA inhibition.

Objective The invalid results and listsmay influence thetimely sending ofthe nucleic acid test reports. This work aimed to explore the reason for the invalid results and to optimize the corresponding operations for safety of blood transfusion. Methods Blood samples were collected from the voluntarydonors in Anyang from Jun 2016 to may 2017and DNAs were extracted withHaoyuan ChiTas BSS1200 automatic nucleic acid separator and subsequently amplified with ABI7500 Fluorescent Amplifier. The invalid results and lists were analyzed. Results A total of 88739 samples were tested for DNAs and 0.06% results and 1.04% lists were found to be invalid. Further analyses of the invalid results showed that it is not the batche's number of reagent but the insufficient amounts of samples as well as personal operation errorsor laboratory contaminationsthat causedthe invalid amplification of internal standard. Conclusion The invalid results and lists can be minimized by enhancing the training of the staff who are involved in nucleic acid tests,improving operating procedures,and avoiding laboratory contaminations by daily cleaning and disinfecting.