Objective SERPINE1 is associated with poor prognosis of many cancers and plays an important role in tumor metastasis. This study aims to clarify the specific regulatory mechanism of miR-532-3p/SERPINE1 in colon adenocarcinoma. Methods TCGA database was used to screen differentially expressed mRNAs, and then mirDIP and miRWalk databases were used to analyze and identify upstream regulatory gene miRNAs. GSEA database was used to the analysis of enrichment pathways. Real time quantitative polymerase chain reaction (qRT-PCR), CCK-8, cloning experiment, scratch healing, Transwell, Western blot (WB) to study the regulatory mechanism of SERPINE1 and its upstream genes on proliferation, migration and invasion of colon adenocarcinoma. Results SERPINE1 was significantly overexpressed in colon adenocarcinoma cells and tissues, while miR-532-3p was significantly overexpressed. SERPINE1 is related to the poor prognosis of colon adenocarcinoma patients and can promote the proliferation, migration and invasion of colon adenocarcinoma cells. SERPINE1 was significantly enriched in ECM RECEPTOR INTERACTION pathway. MiR-532-3p targets and negatively regulates the expression of SERPINE1. The reversion experiment shows that miR-532-3p/SERPINE1 regulates the ECM RECEPTOR INTERACTION pathway and affects the malignant progression of colon adenocarcinoma cells. Conclusion As the upstream regulatory gene of SERPINE1, miR-532-3p inhibits the proliferation, migration and invasion of colon adenocarcinoma cells by regulating ECM RECEPTOR INTERACTION. MiR-532-3p/SERPINE1 has the potential to become a new target for the treatment of colon adenocarcinoma.

In order to further improve and standardize the laboratory diagnosis methods of hemolytic disease of the fetus and newborn（HDFN）and improve the level of clinical diagnosis and treatment, we investigated the laboratory detection of HDFN in 57 medical institutions in China, performed a Meta-analysis of 26 studies including 32 932 maternal and fetal ABO incompatibility cases, and summarized the diagnosis and treatment strategies at home and abroad. We hope to put forward the existing problems of laboratory detection, and discuss new strategy for diagnosis and treatment for HDFN.

Aplastic anemia (AA) is a bone marrow hematopoietic failure disease. Blood component transfusion is necessary to treat anemia and maintain quality of life in AA patients. However, long-term blood transfusion easily leads to transfusion dependence, and the current blood supply is insufficient, and it is also increasingly difficult to meet the blood transfusion needs of AA patients. Therefore, it is particularly important to perform "patient blood management" (PBM) for AA, apply various non-transfusion methods to treat anemia, reduce the transfusion of allogeneic blood, and improve the quality of life of AA patients. Traditional Chinese medicine (TCM) treatment can improve the symptoms of anemia in AA patients, reduce the need for allogeneic blood transfusion, and facilitate the blood management of AA patients. This article reviews the traditional Chinese medicine and its mechanism of action in the treatment of AA, and discusses the clinical significance of traditional Chinese medicine in the blood management of AA patients.

Objective To explore the effectof phosphorylated AMPK and adiponectinresistance on valvular heart disease（VHD） complicated with cardiac insufficiency. Methods A total of 47 VHD inpatients were selected as VHD group，33 cases of cardiac trauma as experimental control，and 50 healthy personsas normal group. ELISA was used to detect the serum adiponectin（APN），tumor necrosis factor-α （TNF-α） and brain natriuretic peptide（BNP）. A small amount of myocardial tissue specimenswas collected bybiopsy in the patients and control group，respectively. APN mRNA expression in cardiac tissue was detected by real time quantitative PCRand APN，AMPK and phosphorylated AMPK （pAMPK） expressions in cardiac muscle tissuesweredetected by Western blotting. Results The serum levels of APN，TNF-α，BNP in VHD group were significantly higher than those in the control and normal group （P<0.05）; serum APN，TNF-α，BNP in control group were obviouslyhigher than thosein normal group （P<0.05）. The myocardial fibers in the tissues of VHDgroup were found to beirregular，with a high expression of APN mRNA and protein （P<0.05）.The expression of pAMPK in VHD group was notablylower than that in the control （P<0.05）. No significant difference of AMPK expressionbetween the VHDpatients and the control （P>0.05）. Conclusion Patients with VHD combined with cardiac insufficiencyshowed increasedserumAPN，TNF-α and BNP but decreasedphosphorylated AMPK，and the APN resistance of myocardial tissuesmay occur，leading to the failure of APN-mediated myocardial protection.

Objective To improve HLA genotyping performance in clinical laboratory,the results of National Proficiency Testing（PT）for HLA-A/B/DRB1 low-resolution genotyping were collected and summarized from 2017 to 2020. Methods HLA genotyping results from 2017~2020 PT were analyzed and different kinds of errors were described and classified,and the possible reasons were also analyzed. Results The number of participant laboratories was increasing from 78 to 96 during 2017 and 2020. 12 096 HLA alleles were returned from the participants during the 4 years and total of 127 errors（1.05%）were found. These 127 errors were classified into two major types including 13 technical errors and 114 artificial errors. The proportion of laboratory which made mistakes was 2.56％（2/78）、4.88％（4/82）、8.75％（7/80） and 3.13％（3/96）in 2017,2018,2019 and 2020,respectively. Conclusion There were above 2.5% of laboratories with errors in HLA genotyping PT surveys past four years. More attentions should be greatly paid to clinical HLA genotyping test and the procedure of reporting.

Objective To realize the statistical analysis of data such as fourfold table chi-square test, evaluation and diagnostic test of four-fold table, and paired design of fourfold table chi-square test through Excel, so as to supplement the statistical function of chi-square test of Excel, and apply it to the teaching practice. Methods The statistical program is compiled through Excel VBA program interface to complete fourfold table chi-square and evaluation and diagnostic test, which compared with the case analysis of Health Statistics to vertify the accuracy of results. Results The input parameters include A (positive number in A rest), B (negative number in A test), C (positive number in B test) and D (negative number in B test). According to the above-mentioned conditions, the software automatically outputs the corresponding statistical description parameters: A+C, B+D, A+B, C, D, A+B+C+D, chi-square test, P values, and judgment. The assessment of diagnostic test and paired chi-square input parameters are: A (true positive), B (false positive), C (false negative) and D (true negative) should be numbers, otherwise there is a prompt dialog box. The software output corresponding statistical description parameters automatically according to these conditions: A+C, B+D, A+B, C+D, A+B+C+D, the diagnostic test's sensitivity, specificity, positive predictive value, negative predictive value and accuracy, matching chi-square test, P values and judgment. Conclusion Excel could replace manual process of fourfold tables data, automatically select the corresponding test statistics method, and provide P values and judgment.

ObjectiveThe study was to investigate the diagnostic values of FDP and D-dimer in patient with acute cerebral infarction(ACI) and deep vein thrombosis(DVT). MethodsThe plasma concentrations of FDP and D-dimer were retrospectively statistically analysed, which included 295 cases of ACI, 84 cases of DVT,and 300 cases without the above- mentioned diseases were set up as control. ResultsFDP and D-dimerin varied in ACI,DVT and the control(P<0.01). Patients with DVT, compared to those with ACI showed a significant increase(P<0.01).Diagnostic sensitivity of FDP and D-dimer was 21.5% and 36.2% in ACI, and 44.1% and 64.2% in DVT,while negative predictive value was 48.8% and 53.8% in ACI,and 86.1% and 90.4% in DVT. ROC curve analysis suggested that FDP and D-dimer had certain diagnostic value in patients with ACI and DVT. ConclusionPlasma markers of FDP and D-dimer can be used as diagnostic marker of acute vascular thromboembolic diseases, particularly in showing higher negative exclusion value in deep venous thrombosis disease.

Objective The study aimed to investigate the relationship between the results of the three hemolysis tests,age on admission,and total serum bilirubin（TSB） in ABO hemolytic disease of the newborn(HDN). Methods Four hundred and eleven cases with ABO HDN admitted to the Affiliated Hospital of Qingdao University between December 2016 and April 2020 were selected. The three hemolysis tests and TSB were tested. We did a retrospective analysis of their clinical data,such as age on admission. Results Among the 411 infants,admission age,positive or negative direct antiglobulin test（DAT）, and positive or negative results for the three hemolysis tests were statistically significant differences（P<0.05） in TSB levels,except for gestational age and birth weight. The rates of positive DAT newborns were 50%,46.67% and 3.33% in admission age groups of ≤1 d,2~4 d and ≥5 d,respectively. The difference was statistically significant（P<0.05）. Excluding cases with gestational age less than 35 weeks and birth weight less than 2 500 g,the correlation coefficient between TSB levels and age on admission was 0.42（P<0.001）. The median TSB levels were 176.37 μmol/L,220.20 μmol/L,and 271.10 μmol/L in age on admission group of ≤1 d, 2~4 d and ≥5 d,respectively. After we applied Bonferroni corrections,the difference was statistically significant（P<0.001）. The older the age on admission,the higher TSB levels in newborns the lower the DAT positive rate. Conclusion For newborns with suspected ABO HDN,the three hemolysis tests are performed in time. Earlier diagnosis and intervention reduce the occurrence of major adverse events.