Objective Identification of a rare blood type of Di(a+b-) with anti-Dib antibody by serological and molecular biology technique. Methods The patient's blood type, direct anti-globulin test, and irregular antibody screening and identification were performed by serology means. DNA sequencing was used to identify the Diego blood type. Results The patient has irregular antibodies against highly prevalent antigens. DNA sequencing confirmed the rare Di(a+b-) blood type. Conclusion Blood typing by DNA sequencing is pivotal for identification of antibodies against highly prevalent antigens.

Objective To compare the consistence of HPV typing tests by using three kits that are domestically available. Methods A total of 82 positive samples and 10 negative samples were collected that had been detected with Liferiver kits in recent 2 months and the Results of HPV typing tests were compared for the agreements with the kits purchased from other two companies of Kaipu and Amply. Results The repeatability of the Results in the three domestic kits were 95.6 %,96.7% and 95.6%,respectively. The consistent rate was 51.0%（47/92）,the partial consistent rate was 23.9%（22/92）,and the inconsistent rate was 25.0%（23/92）.The consistent rate of 86.4%（32/37）was seen in detections of single type infection,22.2%（10/45）in multiple infections,and the partial consistent rate found to be 48.9%（22/45）. Conclusion of the Results in the three domestic kits varied,and the hospital should be fixed for HPV typing before and after treatments of the infection.

Objective To study the changes of laboratory test indexes of pneumonia patients infected by novel coronavirus and its clinical significance to provide scientific reference for clinical diagnosis and treatment. Methods The relevant blood test indexes of 80 patients with new diagnosed novel coronavirus pneumonia admitted to our hospital in 2020 were retrospectively analyzed. Normal physical examination subjects were enrolled as Group A （n=40 cases）, patients with pneumonia suspected of novel coronavirus infection were Group B （n=40 cases） and patients with pneumonia diagnosed as novel coronavirus infection were Group C（n=80 cases）. The changes of laboratory blood routine, T lymphocyte subgroup and biochemical parameters in the three groups were compared. Results Compared with normal group A, WBC, L（%）, RBC, HGB, CD3 ⁺, CD4 ⁺, CD8 ⁺, BUN, UA, in confirmed group C were lower than those in normal group A, with statistical difference（P<0.05）;while G（%）, hs-CRP, ALT, AST, TB, DB, SAA, LDH, CK-MB in confirmed group C were higher than those in normal group A, with statistical difference（P<0.05）.Compared with suspected group B, CD4 ⁺、TnI are lower in confirmed group C than that in suspected group B, AST, SAA, LDH, CK-MB in confirmed group C are higher than those in suspected group B with statistical difference（P<0.05）. There was no significant difference in PLT, CD4 ⁺/CD8 ⁺, CR, CYC, PCT, MYO, CK among the three groups（P>0.05）. Multivariate logistic stepwise regression analysis showed that the higher are in SAA, CK-MB, LDH, AST\TB, DB, the greater is the risk of novel coronavirus pneumonia; The lower are in the WBC, L%, HGB, BUN, UA, CD4 ⁺, CD8 ⁺, the greater is the risk of novel coronavirus pneumonia. Conclusion The changes of various laboratory indexes in patients infected by novel coronavirus have obvious significance. These indexes have certain reference value for the early diagnosis and treatment of novel coronavirus.It is an effective supplement to nucleic acid test（a gold standard method）, which is helpful for clinical diagnosis of novel coronavirus pneumonia.

Objective To investigate the level and clinical value of autoantibodies in patients with malignant pulmonary nodules. Methods Seventy-one patients with asymptomatic pulmonary nodules admitted to our hospital from November 2018 to January 2019 were enrolled, including 58 malignant pulmonary nodules and 13 benign pulmonary nodules. And ten healthy people as control group.The levels of p53, PGP9.5, SOX2, GAGE7, GBU4-5, MAGEA1 and CAGE in all specimens were detected by ELISA kit. The ROC curve was used to analyze the diagnostic value of autoantibodies for malignant pulmonary nodules. Results Compared with the benign pulmonary nodules group and the normal control group, the level of autoantibodies in the malignant nodules of the lungs was significantly increased (P<0.05);ROC curve analysis showed that seven autoantibodies were important for the differential diagnosis of benign and malignant pulmonary nodules (AUC>0.5, P<0.05), and seven autoantibodies combined detection could improve the differential diagnosis efficiency (AUC=0.822);The positive detection rate of lung malignant nodules was 81.7% by LDCT and was increased to 94.0% combining with autoantibodies detection (P<0.05). Conclusion Serum autoantibody detection has potentialclinical value in the differential diagnosis of benign and malignant pulmonary nodules.

Objective To choose a more reliable method to determine the hemoglobin concentration of blood donors in order to further improve the accuracy. Methods On the basis of gold standard method for the detection of hemoglobin (HiCN method), we used blood cell analyzer as the control group. Comparison test was carried out between visual reagent on hemoglobin (copper sulfate proportion) and dry hemoglobin test paper. Results Regression analysis of 151 cases of data samples with confidence degree P =95% was carried out. Comparison of Uritest detection(y) and Sysmex reference(x), y=0.892 2x+15.979, R ²=0.7461; comparison of Hemocue detection(y) and Sysmex reference(x), y=0.949 4x+15.018, R ² =0.838 1. Conclusion Determination of hemoglobin with dry hemoglobin test paper is a method with similar sensitivity and stability with that of the method using blood cell analyzer. The precision, accuracy and linear degree fully meet the needs of testing. It has practical significance in especially blood screening test in blood banks.

Objective To explore the effect of MDSCs on the number and function of CD8+T cells,tumor growth in vivo and survival in Lewis lung cancer mice. Methods LLC tumor cells were inoculated into the flank of C57 LB/6 mice. MDSC were depleted by intravenous administration of Gr-1 blocking antibody. Tail blood was collected and CD8+T cells,MDSCs and splenocyte cytotoxicity of LLC were detected by flow cytometry. Tumor growth and survival of tumor bearing mice were recorded. Results On day 7 after tumor inoculation,the percentages of MDSCs from tumor bearing mice were significantly higher than the control （23.87±1.35% vs 7.600±0.677%,P<0.001） and CD8+T cells were declined compared with the control （7.906±0.428%） vs （11.22±0.606%,P<0.001）. Gr-1 blocking antibody was administered to deplete MDSCs. On day 3 after administration,the percentages of MDSCs were rapidly decreased（1.578±0.299% vs 38.34±3.214%,P<0.001） and CD8+T cells were significantly higher than control mice（9.464±0.820 vs 4.024±0.488,P<0.001）. On day 10 after administration,MDSCs percentages were increased,but decreased when compared to control mice（1.578±0.299%） vs（38.34±3.214%,P=0.0016） and CD8+T cells were deceased but elevated compared with control mice（9.464±0.820%）vs（ 4.024±0.488%,P=0.0019）. Tumor growth in mice with MDSCs depletion was markedly inhibited compared with control group（P<0.05）. The survival of mice with MDSCs depletion was notably longer than control mice（P=0.036,median survival 46 vs 53）. Conclusion MDSCs depletion in LLC tumor bearing mice eliminated the inhibition of CD8+T cells by MDSCs Leading to a restriction of tumor growth and prolonged survival.