MiR-532-3p Targets SERPINE1 and Regulates ECM RECEPTOR INTERACTION to Inhibit Proliferation and Migration of Colon Adenocarcinoma Cells

doi:10.3969/j.issn.1671-2587.2023.03.007

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2023, Vol. 25 ›› Issue (3): 324-333.DOI: 10.3969/j.issn.1671-2587.2023.03.007

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MiR-532-3p Targets SERPINE1 and Regulates ECM RECEPTOR INTERACTION to Inhibit Proliferation and Migration of Colon Adenocarcinoma Cells

LIU Ting, WEN Yanping

Department of Gastrointestinal Surgery, The Central Hospital of Yongzhou, Yongzhou 425000

Received:2023-03-10 Published:2023-07-10

Abstract

Abstract: Objective SERPINE1 is associated with poor prognosis of many cancers and plays an important role in tumor metastasis. This study aims to clarify the specific regulatory mechanism of miR-532-3p/SERPINE1 in colon adenocarcinoma. Methods TCGA database was used to screen differentially expressed mRNAs, and then mirDIP and miRWalk databases were used to analyze and identify upstream regulatory gene miRNAs. GSEA database was used to the analysis of enrichment pathways. Real time quantitative polymerase chain reaction (qRT-PCR), CCK-8, cloning experiment, scratch healing, Transwell, Western blot (WB) to study the regulatory mechanism of SERPINE1 and its upstream genes on proliferation, migration and invasion of colon adenocarcinoma. Results SERPINE1 was significantly overexpressed in colon adenocarcinoma cells and tissues, while miR-532-3p was significantly overexpressed. SERPINE1 is related to the poor prognosis of colon adenocarcinoma patients and can promote the proliferation, migration and invasion of colon adenocarcinoma cells. SERPINE1 was significantly enriched in ECM RECEPTOR INTERACTION pathway. MiR-532-3p targets and negatively regulates the expression of SERPINE1. The reversion experiment shows that miR-532-3p/SERPINE1 regulates the ECM RECEPTOR INTERACTION pathway and affects the malignant progression of colon adenocarcinoma cells. Conclusion As the upstream regulatory gene of SERPINE1, miR-532-3p inhibits the proliferation, migration and invasion of colon adenocarcinoma cells by regulating ECM RECEPTOR INTERACTION. MiR-532-3p/SERPINE1 has the potential to become a new target for the treatment of colon adenocarcinoma.

Key words: Adenocarcinoma of colon, SERPINE1, miR-532-3p, Proliferation, Transfer

CLC Number:

R735.3

LIU Ting, WEN Yanping. MiR-532-3p Targets SERPINE1 and Regulates ECM RECEPTOR INTERACTION to Inhibit Proliferation and Migration of Colon Adenocarcinoma Cells[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2023, 25(3): 324-333.

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MiR-532-3p Targets SERPINE1 and Regulates ECM RECEPTOR INTERACTION to Inhibit Proliferation and Migration of Colon Adenocarcinoma Cells

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