LncRNAs ABHD11-AS1 Targets miR-133a to Upregulate EGFR Expression and Promote Ovarian Cancer cell Proliferation

doi:10.3969/j.issn.1671-2587.2022.05.005

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2022, Vol. 24 ›› Issue (5): 574-581.DOI: 10.3969/j.issn.1671-2587.2022.05.005

Previous Articles Next Articles

LncRNAs ABHD11-AS1 Targets miR-133a to Upregulate EGFR Expression and Promote Ovarian Cancer cell Proliferation

WANG Zhi-feng, SUN Hao, HU Dian, et al

Department of Gynecology and Obstetrics, Second Affiliated Hospital of Naval Medical University, Shanghai 200003

Received:2021-11-26 Online:2022-10-20 Published:2022-10-31

Abstract

Abstract: Objective To investigate the expression of LncRNAs ABHD11-AS1 in ovarian cancer cell and oviductal epithelial cell and explore the effect of LncRNAs ABHD11-AS1 on ovarian cancer cell proliferation. Methods The level of LncRNAs ABHD11-AS1 and miR-133a was detected by Real-time PCR, and interaction between them was confirmed by Dual- Luciferase reporter assay (DR). Cells were transfected with NC siRNA, ABHD11-AS1 as well as siRNA NC mimic, miR-133a mimic and NC inhibitor and miR-133a inhibitor, respectively, and the proliferation was detected by Cell Counting Kit-8 (CCK-8) assay. Real-time PCR and western blotting were performed to detect the EGFR expression. Results Downregulation of lncRNAs ABHD11-AS1 could significantly inhibit the proliferation of ovarian cancer cells. Moreover, lncRNAs ABHD11-AS1 was negatively correlated with the expression of miR-133a. DR results showed that luciferase activity was significantly reduced in cells transfected with miR-133a mimic and wild-type ABHD11-AS1 fragments; and the level of miR-133a was lower in ovarian cancer cell compared to oviductal epithelial cells. Transfection of miR-133a mimic significantly upregulated miR-133a expression in both of these cells and inhibited their proliferation and EGFR expression. In addition, downregulating miR-133a significantly promoted the proliferation of ovarian cancer cells and increased the EGFR expression, while downregulating LncRNAs ABHD11-AS1 significantly decreased the EGFR expression. Conclusion LncRNAs ABHD11-AS1 promoted the malignant behaviors of ovarian cancer by targeting miR-133a to upregulate its downstream target gene EGFR.

Key words: LncRNAsABHD11-AS1, MiR-133a, Ovarian cancer, Proliferation

CLC Number:

R737.31

WANG Zhi-feng, SUN Hao, HU Dian, et al. LncRNAs ABHD11-AS1 Targets miR-133a to Upregulate EGFR Expression and Promote Ovarian Cancer cell Proliferation[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2022, 24(5): 574-581.

References

[1] SLATNIK C L P,DUFF E. Ovarian cancer[J]. Nurse Pract,2015,40(9):47-54.
[2] KUJAWA K A,LISOWSKA K M.Ovarian cancer—from biology to clinic[J]. Postepy Hig Med Dosw (Online),2015,69:1275-1290.
[3] HENDERSON J T,WEBBER E M,SAWAYA G F.Screening for ovarian cancer:updated evidence report and systematic review for the US preventive services task force[J]. JAMA,2018,319(6):595-606.
[4] MENON U,KARPINSKYJ C,GENTRY-MAHARAJ A.Ovarian cancer prevention and screening[J]. Obstet Gynecol,2018,131(5):909-927.
[5] CHIEN J,POOLE E M.Ovarian cancer prevention,screening,and early detection:report from the 11th biennial ovarian cancer research symposium[J]. Int J Gynecol Cancer,2017,27(9S Suppl 5):S20-S22.
[6] ZHOU Y,JIN Z J,WANG C C.Glycogen phosphorylase B promotes ovarian cancer progression via Wnt/β-catenin signaling and is regulated by miR-133a-3p[J]. Biomed Pharmacother,2019,120:109449.
[7] ZHANG G L,PIAN C,CHEN Z,et al.Identification of cancer-related miRNA-lncRNA biomarkers using a basic miRNA-lncRNA network[J]. PLoS One,2018,13(5):e0196681.
[8] ZHONG G S,LOU W Y,YAO M Y,et al.Identification of novel mRNA-miRNA-lncRNA competing endogenous RNA network associated with prognosis of breast cancer[J]. Epigenomics,2019,11(13):1501-1518.
[9] ZENG X Y,JIANG X Y,YONG J H,et al.lncRNA ABHD11-AS1,regulated by the EGFR pathway,contributes to the ovarian cancer tumorigenesis by epigenetically suppressing TIMP2[J]. Cancer Med,2019,8(16):7074-7085.
[10] LEI X Y,LI L C,DUAN X Y. Long non-coding RNA ABHD11-AS1 promotes colorectal cancer development through regulation of miR-133a/SOX4 axis[J]. Biosci Rep,2018,38(6):BSR20181386.
[11] ACUNZO M,ROMANO G,WERNICKE D,et al.microRNA and cancer—a brief overview[J]. Adv Biol Regul,2015,57:1-9.
[12] NAM E J,YOON H,KIM S W,et al.microRNA expression profiles in serous ovarian carcinoma[J]. Clin Cancer Res,2008,14(9):2690-2695.
[13] LUO J,ZHOU J H,CHENG Q,et al.Role of microRNA-133a in epithelial ovarian cancer pathogenesis and progression[J]. Oncol Lett,2014,7(4):1043-1048.
[14] PENG W X,KOIRALA P,MO Y Y.LncRNA-mediated regulation of cell signaling in cancer[J]. Oncogene,2017,36(41):5661-5667.
[15] XIN H R,YAN Z F,CAO J.Long non-coding RNA ABHD11-AS1 boosts gastric cancer development by regulating miR-361-3p/PDPK1 signalling[J]. J Biochem,2020,168(5):465-476.
[16] LIU Y,WANG L L,CHEN S,et al.LncRNA ABHD11-AS1 promotes the development of endometrial carcinoma by targeting cyclin D1[J]. J Cell Mol Med,2018,22(8):3955-3964.
[17] ZHANG W,HUANG X,SHI J G.EZH2-mediated lncRNA ABHD11-AS1 promoter regulates the progression of ovarian cancer by targeting miR-133a-3p[J]. Anticancer Drugs,2021,32(3):269-277.
[18] LIU B W,WANG W,SUN S F,et al.Knockdown of lncRNA ABHD11-AS1 suppresses the tumorigenesis of pancreatic cancer via sponging miR-1231[J]. Onco Targets Ther,2020,13:11347-11358.
[19] LAGOS-QUINTANA M,RAUHUT R,YALCIN A,et al.Identification of tissue-specific microRNAs from mouse[J]. Curr Biol,2002,12(9):735-739.
[20] FENG Y,NIU L L,WEI W,et al.A feedback circuit between miR-133 and the ERK1/2 pathway involving an exquisite mechanism for regulating myoblast proliferation and differentiation[J]. Cell Death Dis,2013,4(11):e934.
[21] TANG Y B,PAN J C,HUANG S,et al.Downregulation of miR-133a-3p promotes prostate cancer bone metastasis via activating PI3K/AKT signaling[J]. J Exp Clin Cancer Res,2018,37(1):160.
[22] KINOSHITA T,NOHATA N,FUSE M,et al.Tumor suppressive microRNA-133a regulates novel targets:moesin contributes to cancer cell proliferation and invasion in head and neck squamous cell carcinoma[J]. Biochem Biophys Res Commun,2012,418(2):378-383.
[23] SHI W Y,TANG T T,LI X P,et al.Methylation-mediated silencing of miR-133a-3p promotes breast cancer cell migration and stemness via miR-133a-3p/MAML1/DNMT3A positive feedback loop[J]. J Exp Clin Cancer Res,2019,38(1):429.
[24] GAO L,LI S H,TIAN Y X,et al.Role of downregulated miR-133a-3p expression in bladder cancer:a bioinformatics study[J]. Onco Targets Ther,2017,10:3667-3683.
[25] GAO S H,LIU J,ZHANG H J,et al.Low miR-133a expression is a predictor of outcome in patients with esophageal squamous cell cancer[J]. Eur Rev Med Pharmacol Sci,2016,20(18):3788-3792.
[26] LI W F,CHEN A Q,XIONG L L,et al.miR-133a acts as a tumor suppressor in colorectal cancer by targeting eIF4A1[J]. Tumour Biol,2017,39(5):1010428317698389.
[27] SONG X S,SHI B,HUANG K X,et al.miR-133a inhibits cervical cancer growth by targeting EGFR[J]. Oncol Rep,2015,34(3):1573-1580.
[28] WANG L K,HSIAO T H,HONG T M,et al.microRNA-133a suppresses multiple oncogenic membrane receptors and cell invasion in non-small cell lung carcinoma[J]. PLoS One,2014,9(5):e96765.
[29] ZHENG L,KANG Y,ZHANG L,et al.miR-133a-5p inhibits androgen receptor (AR)-induced proliferation in prostate cancer cells via targeting FUsed in Sarcoma (FUS) and AR[J]. Cancer Biol Ther,2020,21(1):34-42.
[30] CUI W J,ZHANG S,SHAN C L,et al.microRNA-133a regulates the cell cycle and proliferation of breast cancer cells by targeting epidermal growth factor receptor through the EGFR/Akt signaling pathway[J]. FEBS J,2013,280(16):3962-3974.
[31] ROSKOSKI R Jr.Small molecule inhibitors targeting the EGFR/ErbB family of protein-tyrosine kinases in human cancers[J]. Pharmacol Res,2019,139:395-411.
[32] TAKEDA M,NAKAGAWA K.First- and second-generation EGFR-TKIs are all replaced to osimertinib in chemo-naive EGFR mutation-positive non-small cell lung cancer?[J]. Int J Mol Sci,2019,20(1):146.

LncRNAs ABHD11-AS1 Targets miR-133a to Upregulate EGFR Expression and Promote Ovarian Cancer cell Proliferation

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 4

Recommended Articles

Metrics

Comments

About

Author

Journal

Access Statistics

Contact

[1]	SU Hong-e, ZHONG Qing-ji, FU Chun-li, et al. Expression and Clinical Significance of MiR-222-3p, HE4 and CA 125 in Serum of Epithelial Ovarian Cancer [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(3): 371-375.
[2]	SHI Lan, XU Liang-fei, NIE Zheng-chao, et al. Study on the Inhibitory Mechanism of Staphylococcus Aureus Leukocidin S Component LukS-PV on the Proliferation in Acute Myeloid Leukemia Cells [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2020, 22(6): 603-609.
[3]	ZHAO Xing-yun, BIAN Mao-hong. Advances in Research on Relationship between MicroRNAs and Erythroid Growth, Proliferation, Differentiation and Apoptosis [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2020, 22(4): 445-448.
[4]	WANG Yun-jie, ZHAO Shao-jie. The MiR-31 and Efficacy of the Combined Chemotherapy of Paclitaxel with Cisplatin: A Retrospective Study in Elderly Females with Ovarian Cancer [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2019, 21(3): 236-238.