Objectives To explore the possibility to utilize unrelated donors' cells as sources for adoptive T cell anti-tumor therapy, thus we need to identify HLA restriction for neoantigens and evaluate neoantigen induced reactive T cells' cytolysis ability. Methods 16 neoantigen peptides were used to induce 18 unrelated donors' (group 1) PBMC (peripheral blood mononuclear cells) into reactive T cells, and HLA typing was performed, the affinity between peptides' and HLA molecule was predicted by NetMHC database, then neoantigen with HLA-A2 restriction was selected to induce another group (group 2) of 17 unrelated donors' PBMC into reactive T cells, these cells would act as effectors, tumor cell line T2 cells or the tumor cells with the same source of this neoantigen would act as targets either, LDH release tests and RTCA (real time celluar analysis) were performed to evaluate the effectors' anti-tumor ability. Then these results were compared between HLA-A2+ and HLA-A2- samples. Results NetMHC database predicted neoantigen LM7 showed high affinity with HLA-A2+ antigens, 5/11 of HLA-A2+ samples can successfully be induced into reactive T cells, including 3/3 homozygous HLA-A2+ samples, while 2/7 of HLA-A2- samples with more reactive T cells. Induced T cells from A2+ samples showed higher percentages of tumor cells killed than those A2- samples(60.72±11.28 vs 47.2± 4.46, P=0.03). Conclusions Our study suggests prediction by NetMHC is more helpful in homozygous samples, neoantigens LM7 is confirmed as HLA-A2 restrictive, which can induce some HLA-A2+ PBMC into reactive T cells which can kill tumor cells more efficiently, unrelated donors should be screened not only by HLA-typing but also cell function tested, thus induced reactive T cells can take the roles as the source for adoptive anti-tumor T cells therapy.

Objectives To assess the recover cells from Leukocyte reduction filters (LRFs) and their related factors, this study analyzed the number of natural killer (NK) cells and T cells from LRFs and whole blood (WB). The correlation between the number of these cells and age, sex and other factors was further analyzed. Methods WB and LRFs (400 mL) were collected from 51 repeat blood donors in Shenzhen Blood Center from July 2023 to June 2024. White blood cells (WBCs) were obtained by washing LRFs with PBS buffer. The cells from WB and LRFs were counted by blood cell analyzer. Mononuclear cells were isolated by density gradient centrifugation. The proportions of CD3 ^-CD56 ⁺NK, CD3 ⁺CD4 ⁺T and CD3 ⁺CD8 ⁺T cells were detected by flow cytometry. Statistical analysis was performed using GraphPad Prism v.9.0 software. Results In a certain flush volume, the number of total lymphocytes from LRFs increased with the increase of flush volume, but the cell density decreased with the increase of flush solution. There was no significant difference in the total number of lymphocytes from LRFs among donors of different ages and genders ( P>0.05). According to the results of cell number and cell ratio, the number of CD3 ^-CD56 ⁺NK cells from LRFs was different in age groups ( P<0.05), but no significant difference was found in gender groups ( P>0.05). The number of CD3 ^-CD56 ⁺NK cells from WB in male donor were significantly higher than that in female donor ( P>0.05). Whether from LRFs or WB, there was no significant difference in the number of CD3 ⁺CD4 ⁺T cells and CD3+CD8+T cells in different age groups and gender groups ( P>0.05). Conclusion In a certain flush volume, the total number of lymphocytes from LRFs was correlated with the volume of the flush solution. The number of CD3 ⁺CD4 ⁺T cells and CD3 ⁺CD8 ⁺T cells were not significantly correlated with the age and gender of blood donor, while the number of CD3 ^-CD56 ⁺NK cells was correlated with the age of blood donor.

Objectives To evaluate the effect of virus reduction in plasma using LED white light. Methods Methylene blue was added to plasma at a final concentration of 1 µM. The activity of coagulation factors in plasma and the inactivation effect on Sindbis virus were compared by different treatment methods (10 cm/5 cm spacing, unilateral/bilateral irradiation), different light intensities (30 000 lx, 35 000 lx, 40 000 lx, 45 000 lx, and 50 000 lx) and different time points (10, 20, and 30 min) using LED white light as the light source. Results Increase time of irradiation led to increase of plasma temperature under 50 000 lx light intensity. The extent of increase was found to be greater at 5 cm than at 10 cm spacing, and greater with bilateral than unilateral irradiation. The 5 cm/10 cm spacing and unilateral/bilateral irradiation had no significant differences in their effect on coagulation factor Ⅷ and fibrinogen (FIB) activity. The 10 cm bilateral spacing was selected as the device parameter for photochemical treatment. Plasma was irradiated at intensities of 30 000, 35 000, 40 000, 45 000, and 50 000 lx, respectively. The results showed Sindbis virus in plasma was inactivated after 5 minutes of irradiation at different light intensities, with titer reduction >4 LogTCID ₅₀/0.1 mL. Factor Ⅷ and FIB activities were retained at the level of 70% and 60%, respectively after 20 minutes of irradiation. Correlation analysis revealed that there was no correlation between different light intensities and loss of factor Ⅷ activity. However, the effect of light intensities on FIB activity was more pronounced within 20 minutes of irradiation. A significant decline in the FIB activity was observed with increasing light intensities, although no significant difference was found after 30 minutes of irradiation. The irradiation time was found to be significantly correlated with the loss activity of coagulation factors, regardless of the light intensities. Conclusion LED white light can act as a new light source for methylene blue virus reduction. Under the right conditions it reduced viruses effectively and preserves the activity of the coagulation factors in plasma.

Objectives To study the clinical efficacy and safety of ABO incompatible platelet transfusion in emergency situations. Methods 271 cases of ABO incompatible platelet transfusion in our hospital from January 2020 to January 2024 were collected, and 128 cases were enrolled which were divided into bidirectional mismatch group (10 cases), major mismatch group (56 cases), and minor mismatch group (62 cases). All 128 patients had ABO homologous platelet transfusion history before and within 10 days after ABO incompatible platelet transfusion. The incompatible transfusion was set as the ABO blood group incompatible experimental group (subgroup B), the previous ABO homozygous platelet transfusion was set as the ABO homozygous control group (subgroup A), and the subsequent ABO homozygous platelet transfusion was set as the ABO homozygous experimental group (subgroup C). Platelet count change after platelet transfusion (△PLT) and 24 h platelet corrected count Increment (CCI) were used to evaluate the clinical effect of platelet transfusion, and the safety of platelet transfusion was assessed by the presence or absence of hemolytic transfusion reactions. Results In the bidirectional mismatch group, there was no significant difference in △PLT and CCI among subgroups A, B and C ( P>0.05). In the major mismatch group, the △PLT and CCI of subgroup B were significantly lower than those of subgroups A and C ( P<0.05), but there was no significant difference between subgroup A and C ( P>0.05). In the minor mismatch group, there was no significant difference in △PLT and CCI among subgroups A, B and C ( P>0.05). There was no acute hemolytic transfusion reactions in any of the ABO incompatibility infusion. Conclusion The clinical effect of ABO major mismatch platelet transfusion is obviously weaker than that of ABO compatible platelet transfusion, but did not affect the outcome of subsequent ABO homozygous platelet transfusion. The clinical effect of ABO minor mismatch platelet transfusion is similar to that of ABO compatible platelet transfusion, but could potentially affect the outcome of subsequent ABO homozygous platelet transfusions. No significant hemolytic transfusion reaction was found by ABO incompatible platelet transfusion.

Objectives To clarify whether NLRP3 inflammasome can modulate macrophage phagocytic capacity towards red blood cells (RBCs) and regulate macrophage subtype polarization. Methods THP-1 cells with low expression of NLRP3 (ID3 THP-1), cells transfected with an empty vector (shNC THP-1), and wild-type THP-1 cells were used as macrophage models. These cells were incubated with untreated RBCs, water bath-aged RBCs, and IgG-opsonized RBCs, respectively. Flow cytometry was used to detect the phagocytic rate of THP-1 cells to different RBC types and to measure the expression of M1 subtype markers (CD16 and CD86), and M2 subtype markers (CD163 and CD206) on THP-1 cells. Results ID3 THP-1 with reduced NLRP3 expression exhibited significantly downregulated phagocytic capacity towards RBCs. Aged RBCs induced the differentiation of THP-1 cells into the M1 subclass while inhibiting their differentiation into the M2 subclass. Decreased expression of the NLRP3 inflammasome in THP-1 cells led to a downregulation of their ability to differentiate into the M1 subclass following RBC phagocytosis, accompanied by their enhanced capacity to differentiate into the M2 subclass. Conclusion NLRP3 inflammasome can serve as a pivotal regulatory target, governing macrophage phagocytosis of RBCs and their subsequent subclass differentiation.

Objectives To investigate the effects of 4 ℃ cold-stored platelets (cold storage platelets, CSP) transfusion on acute kidney injury caused by hemorrhagic shock/resuscitation (HS/R) in rats. Methods 20 healthy male SD rats were randomly divided into 4 groups: sham operation group (blank group), HS/R model group (model group), CSP transfusion group, room temperature-stored platelets (RTP) kept at 22 ℃ transfusion group (RTP transfusion group). The rats were executed 24 hours after volume resuscitation, and blood and kidney tissue specimens were collected. Serum concentrations of creatinine (Scr), urea nitrogen (BUN) , cystatin C (CysC) and interleukin-1β (IL-1β) and interleukin-18 (IL-18) in renal tissue were detected in each group. The expression levels of neutrophil gelatinase-associated lipid carrier protein (NGAL) and renal injury cause-1 (KIM-1) in renal tissue were detected by Western Blot method, and the pathological changes of renal tissue were observed by HE staining. Results ①There were no significant differences in baseline body mass, MAP, SCr, BUN and other indexs among four groups ( P>0.05). ②24 h after resuscitation, the levels of SCr ( P<0.05), BUN ( P<0.01), CysC ( P<0.01) were higher in the model group than in the other three groups; the levels of BUN ( P<0.01), SCr ( P=0.037), CysC ( P=0.020) in the CSP infusion group were lower than those in the RTP infusion group. ③Compared with the model group, serum IL-1β and IL-18 levels were significantly lower in both CSP infusion group and RTP infusion group. Compared with the RTP infusion group, serum IL-1β levels were reduced in the CSP infusion group ( P=0.041). Compared with model group, NGAL protein expression was significantly decreased in CSP infusion group ( P<0.01), and KIM-1 protein expression was significantly decreased in both CSP infusion group and RTP infusion group ( P<0.01). Renal histopathological results showed that CSP transfusion could reduce the degree of renal cell degeneration, necrosis and inflammatory cell infiltration. Conclusion Compared with RTP, CSP transfusion could reduce HS/R acute kidney injury in SD rats.

Whole blood was the earliest blood product ever used, however, with the advent of component transfusion treatments, whole blood all but disappeared from blood bank lists in the 1970 s. In recent years, based upon the successful military experience, the use of whole blood to resuscitate patients with hemorrhagic trauma has again attracted the attention of the blood transfusion circle,and it has gradually been introduced from the military field to the civilian treatment settings.Based on the guidelines for whole blood transfusion at home and abroad and related published studies, this paper reviews the classification of whole blood, history of whole blood use, whole blood storage and platelet protection, removal of whole blood white blood cells, inactivation of whole blood pathogens, and urgent problems to be solved in scientific and accurate transfusion of whole blood, and looks forward to its future development direction, providing reference for the introduction of whole blood more widely, and the development of blood transfusion medicine.

Objective To know the frequency of HLA antibodies in donors in Shanghai area, to provide basic data for the research on TRALI. Methods Samples of blood donors from October 2020 to April 2021 were randomly selected to detect HLA-specific antibodies by flow cytometry and microbead method, and the incidence of HLA antibodies was statistically analyzed. Results In 9 797 serum samples of blood donors, 1 715 (17.51%) were positive for HLA antibodies, among which the frequency of HLA antibodies in males and females was 4.81% and 28.08%. Excluding the history of blood transfusion, the frequency of HLA antibodies in women with no pregnancy history and women with pregnancy history was 12.10% and 36.62%, and comparative analysis the group of once, twice and three or more times pregnancies donors population, which frequency of HLA antibodies was 29.97%, 40.70% and 44.80%. The frequency of HLA antibodies in female donors with multiple pregnancy history was significantly higher than that in single pregnancy ( P<0.05). Conclusion Consultation of pregnancy history and HLA antibodies detection of female blood donors in blood stations can prevent from the occurrence of TRALI.

Objective To evaluate the differential biological effects of human serum albumin (HSA) derived from different sources on vascular endothelial cells. Methods Human umbilical vein endothelial cells (HUVEC) were serum-starved and then exposed to HSA from varied sources. Cellular responses were assessed using the CCK-8 assay for proliferation, Annexin V-FITC kit for apoptosis, and flow cytometry for cell cycle analysis. The proliferative, apoptotic and cell cycle effects of HSA from different sources were statistically compared. Additionally, the impact of HSA on HUVEC cell migration and tube formation was assessed via scratch assays and tube formation experiments. Results Yeast-derived rHSA1 did not affect HUVEC proliferation ( P=0.49, q=1.601), while other HSA sources significantly promoted it ( P<0.001, F=10.84). All HSA treatment groups exhibited an inhibitory effect on HUVEC apoptosis ( P<0.001), with no statistically significant differences in the proportions of live cells ( P=0.07, F=2.415), apoptotic cells ( P=0.2, F=1.624), and dead cells ( P=0.28, F=1.376) across treatment groups. Compared to the serum-free control group, except for the pHSA2 and pHSA6 treatment groups, which showed a significant decrease in G0/G1 phase cell proportion ( P<0.001) and a significant increase in G2/M phase cell proportion ( P<0.01), the proportions of cells in other cell cycle phases did not show significant changes in the HSA treatment groups. In the scratch assay, in contrast to the serum-free control group, the pHSA(1) and pHSA(2) groups exhibited a higher degree of wound healing at 12 h ( P<0.001), 24 h ( P<0.001), and 36 h ( P<0.001, P=0.002); however, no significant differences were observed in the rHSA(1) and rHSA(2) groups compared to the control group. Likewise, in the tube formation assay, the pHSA(1) and pHSA(2) groups significantly enhanced node formation ( P=0.001, P=0.005), tubular branching ( P<0.001), and mesh structure development ( P<0.001), whereas the rHSA(1) and rHSA(2)groups showed no such enhancements. Conclusion HSA from different sources significantly inhibits endothelial cell apoptosis under serum-free conditions, but exerts varying effects on proliferation, cell cycle regulation, cell migration, and tube formation of vascular endothelial cells.

Non-transfusional hemotherapy should be mainly carried out by the Transfusion Department, which basis is the apheresis technology by centrifugation. The plasmapheresis can be realized in two ways: centrifugal and membrane filtration, each of which has its own technical characteristics. Apheresis/blood purification based on centrifugation shows the advantages of higher plasma separation efficiency, shorter treatment time, less platelet loss, less destruction of red blood cells, and the use of citrate anticoagulation for non-continuous clinical treatment of critically ill patients. Secondary columns suitable for centrifugal technology can realize immunoadsorption, artificial liver support system, centrifugation-filtration plasmapheresis and inflammatory factor adsorption. Using increasingly sophisticated secondary column technology should be a useful supplement to traditional TPE.

Objective Detecting the plasma vascular cell adhesion molecule 1 (VCAM-1) level in neonates with ABO hemolytic disease (HDN), to predict the degree of endothelial damage and in vivo hemolysis in ABO-HDN children. Methods A total of 127 cases of ABO-HDN attending our hospital between June 2022 and June 2023 were retrospectively collected, and further divided into three subgroups, namelymild, moderate, and severe hyperbilirubinemia. 127 healthy newborns with matching maternal and infant blood groups were recruited as a healthy control group. 41 cases of non-hemolytic jaundice were set up as the control group. A triple hemolytic test clarified the diagnosis of ABO-HDN, and all samples were tested for blood type and irregular antibodies. Plasma VCAM-1 were determined by enzyme-linked immunosorbent assay. Results 1 There was no statistically significant difference between the ABO-HDN group and the healthy control group regarding the sex of the newborns, birth weight, blood type, mode of delivery of their mothers, and the presence or absence of preterm rupture of membranes ( P>0.05), and the neonatal gestational age, maternal age, and number of pregnancies showed significant differences between the two groups ( P<0.05). There were differences in hemoglobin (Hb), reticulocyte (Ret), indirect bilirubin (IBIL), lactate dehydrogenase (LDH), high-sensitivity C-reactive protein (hs-CRP) and plasma VCAM-1 ( P<0.05, and the levels of VCAM-1 were positively correlated with the levels of LDH, IBIL, and Ret, and negatively correlated with the levels of Hb ( P<0.05). VCAM-1 levels in the ABO-HBN group showed independent correlations with Hb, Ret, IBIL, and LDH levels ( P<0.05). Among the three subgroups, VCAM-1 levels were significantly higher in the severe hyperbilirubinemia group than in the mild and moderate hyperbilirubinemia groups ( P<0.05). Conclusion Elevated VCAM-1 in children with ABO-HDN may be associated with vascular endothelial damage and with help in assessing the severity of hemolysis in the disease.

Cell-free fetal DNA (cff-DNA) exists in the peripheral blood of pregnant women during gestation, and it carries DNA fragments with relevant genetic information of the fetus, which can be screened for fetal chromosomal and gene-related diseases. It is now widely used in non-invasive prenatal testing (NIPT) because of its low operational risk and lack of side effects. Non-invasive cff-DNA blood group testing uses molecular technology to detect the genes associated with cff-DNA blood grouping, resulting in a fetal blood group. The test can be used to detect the consistency of fetal and maternal blood groups during pregnancy and to determine the risk of hemolytic disease of the fetus and newborn (HDFN) due to blood group incompatibility.

The mammalian target of rapamycin (mTOR) is a serine/threonine protein kinase that plays an important role in regulating cell growth, proliferation.The mTOR complex regulates cell growth, proliferation, protein metabolism by phosphorylating and activating downstream substrates such as S6K1, 4E-BP, etc. The mTOR signalling pathway similarly plays an important role in the haematopoietic system, integrating multiple signals to regulate the three processes of haematopoietic stem cell quiescence, self-renewal and multidirectional differentiation.This article systematically describes how relevant signalling molecules and proteins affect the mTOR signalling pathway and further influence haematopoietic stem cell function.

In recent years, the mechanisms of platelet aging have garnered increasing attention. With advancements in detection technologies, researchers are now able to study the age stratification of platelets in the peripheral blood of healthy individuals, revealing extensive changes at both the transcriptomic and proteomic levels during the aging process. Pathophysiological studies have further elucidated the critical role of platelets in various diseases, closely associated with age-related changes in platelets. However, the specific characteristics of platelet aging and their precise role in disease development remain to be fully elucidated. Therefore, understanding the physiological characteristics and molecular mechanisms of aging platelets under normal conditions is crucial for research. This review summarizes the transcriptomic and proteomic studies on platelet aging in physiological conditions, as well as the changes in platelet turnover in related diseases, aiming to provide a reference for exploring the relationship between platelet aging and disease development.

Objective To provide new insights into the prevention and treatment of human cytomegalovirus (HCMV) by observing the effect of autophagy induced by oxidative stress on the proliferation of HCMV in cells. Methods Using PCR to amplify the LC3B encoding fragment, an autophagy expression vector was constructed to observe the formation of cellular autophagy. Then, HFF cells infected with HCMV (AD169) for 72 hours were collected. The levels of UL122 expression and viral particles were detected by real-time quantitative PCR. And the viral protein pp65 expression level and the proliferation of virion were detected by Western blot and TCID ₅₀, respectively. Results Autophagy detection vectors were successfully constructed, which could be used to indicate the formation of cellular autophagy. Compared with normal cultured cells, oxidative stress could induce autophagy formation, and upregulated UL122 gene expression, pp65 protein levels and viral load by this pathway. The viral titer test also showed that autophagy could promote the replication of HCMV in cells. Conclusion Oxidative stress can induce autophagy and promote the replication and proliferation of HCMV, while autophagy inhibitor 3-MA can inhibit the replication of HCMV promoted by oxidative stress. It is confirmed that autophagy is one of the mechanisms of oxidative stress promoting the replication and proliferation of HCMV.

In recent years, research on immune substances in red blood cells has shown that red blood cells are not only the main carrier of oxygen transportation, but also play a variety of immune and regulatory functions as innate immune cells. These cells are capable of recognizing and adhering to antigens, fostering phagocytosis, engaing with complement system, and eliminating circulating immune complexes. Immune substances on its surface, such as Toll-like receptor 9(TLR9), complement receptor 1 and CD58, can act as bridges to maintain the immune balance of the body. Exploring the role of red blood cells with immune characteristics and their cytokines in the development and progression of diseases has important clinical significance.

Platelets play a crucial role in a variety of physiologic and pathologic processes, especially in hemostasis and wound healing. In recent years platelet-rich plasma, platelet-rich fibrin, platelet-lysate and other platelet-derived products have become a promising treatment in regenerative medicine and have been widely applied clinically. By release a variety of growth factors, cytokines, and chemokines, platelet-derived products induce cell migration, proliferation, differentiation, and chemotaxis, stimulating mitosis in multiple cell types and neovascularization, increasing endothelial cell response to pro-angiogenic factors, and promoting fibroblast migration and proliferation. This article reviews the classification and origin of platelet-derived products, biological characteristics of the main growth factors, and their therapeutic effects in promoting the healing of various wounds, then, also analyzed the possible issues and the future development possibilities.

Cellular immunotherapies, new complementary therapies for tumor patients, are aimed at malignant tumors with low ability of autoimmune cells, including T cell-based chimeric antigen receptor T cell (CAR-T) therapy, tumor infiltrating lymphocytes (TILs) therapy, NK cell-based chimeric antigen receptor NK cell (CAR-NK) therapy, and autologous cell immunotherapy (CIK), as well as infusion therapies based on other immune cells such as mononuclear phagocytes like dendritic cells and macrophages. Among them, nature killer cells (NK cells), important to the body's natural immunity, matter greatly in anti-tumor, antiviral infection and immune regulation. Like T cells, it can be engineered to target tumor treatment, and can also be transfused with allogeneic NK cells, making up for the shortcomings of limited T cell source and allogeneic immune rejection. No evidence shows that patients tansfused with allogeneic NK cells may develop severe Graft Versus Host Disease (GVHD). NK cells not only expand the types of cells used in cellular immunotherapy, but also provide a broad application prospect for the formation of low-cost cellular immunotherapy products. However, there are still problems such as unstable quality of NK cells and lack of unified quality standards in the preparation process. Although some NK cell immunotherapy products have been approved by the Food and Drug Administration (FDA) or the National Medical Products Administration (NMPA), there is still no clear and publicly available standardized production system for NK cell immunotherapy products. Starting from the unique immunomodulatory mechanism of NK cells, this paper summarizes the therapeutic strategies applied by researchers in the treatment of malignant tumors in recent years and the latest progress of preclinical studies and clinical trials, and finally focuses on the research progress of in vitro expansion methods and maintenance of active function of NK cells, indicating that NK cells promise to be a unified quality of cellular immunotherapy products.

Objective To explore the feasibility and risks of using extracellular vesicles (EVs) secreted by tolerant dendritic cells (DC) to intervene in transfusion-related acute lung injury (TRALI). Methods Mouse bone marrow-derived DC cells were induced in vitro to become tolerant DCs by treatment with rapamycin, and the culture supernatant was collected and EVs were harvested by gradient centrifugation. Balb/c mice were induced into a TRALI disease model using LPS and anti-H2Kd antibodies, and EVs secreted by isogenic rapamycin-DCs or allogenic rapamycin-DCs were used to intervene in TRALI mice, and a series of data of diseased mice were observed and recorded. Results Compared with the TRALI onset group, after using rapamycin-treated tolerant DCs to intervene in TRALI onset mice, the mortality rate of the mice was significantly reduced. Unexpectedly, intervention with EVs generated from allogeneic tolerant DC (after rapamycin treatment) aggravated the mortality of TRALI. Despite EVs intervention, lung wet-to-dry ratio, pleural effusion and body temperature were not significantly different from those in mice treated with LPS and H2Kd antibodies alone. However, the mortality rate after EVs intervention was positively correlated with the injection concentration, even though the allogeneic vesicles were derived from tolerant cells and did not induce TRALI in mice when combined with LPS. Conclusion Allogeneic extracellular vesicle infusion combined with anti-leukocyte antibodies will increase the risk of death in TRALI, even though autologous DC has a protective effect on TRALI. Also, the allogeneic extracellular vesicles only combined with LPS does not cause respiratory distress. The data from this study suggest that we need to pay more attention on the risk of TRALI aggravated by the application of allogeneic EV therapies.

Objective Through the research of many cases of mimicking antibodies, To study the characteristics of mimicking antibodies and explore the transfusion principles of mimicking antibodies. Provide effective detection methods and clinical suggestions for patients with mimicking antibodies. Methods A retrospective analysis was made on the sex, age, diagnosis and serological test of 160 cases of mimicking antibodies. Analyze the experimental data and find the relevant rules. Results The specificity of RH blood group antibodies is the most common in all mimicking antibodies. Mimicking antibodies of anti-Ce, anti-Ec and anti-D accounted for 94.48% in 160 cases. The type of CCDee is the most common type in all cases. GEL test in serum showed strong antibody reactivity (the score was 7.16). Indirect antiglobulin test in serum with tube showed strong specificity. Conclusion It is suggested that a variety of identification methods should be employed because it's difficult to identify the specificity of mimicking antibodies. The GEL test is more reactive in detecting antibodies and Indirect antiglobulin test method is more specific in distinguishing antibodies. The two methods should be combined. Blood transfusion treatment should be as far as possible to avoid mimicking antibody specificity, transfuse corresponding antigen negative of red blood cells will have obvious therapeutic effect on the patients.