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JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2024, Vol. 26 ›› Issue (6): 744-749.DOI: 10.3969/j.issn.1671-2587.2024.06.005

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Differential Expression and Clinical Significance of Vascular Cell Adhesion Molecule-1 in Plasma of Neonates with ABO Hemolytic Disease

SHEN Qianyun1,2, CHENG Wenguo2, HOU Shuning2, YAO Genghong1   

  1. 1Department of Rheumatology and Immunology, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, Nanjing, Jiangsu 210001;
    2Department of Clinical Laboratory, Yangzhou Maternal and Child Health Hospital, Affiliated Hospital of Medical School of Yangzhou University, Yangzhou, Jiangsu 225002
  • Received:2024-04-23 Online:2024-12-20 Published:2024-12-20

Abstract: Objective Detecting the plasma vascular cell adhesion molecule 1 (VCAM-1) level in neonates with ABO hemolytic disease (HDN), to predict the degree of endothelial damage and in vivo hemolysis in ABO-HDN children. Methods A total of 127 cases of ABO-HDN attending our hospital between June 2022 and June 2023 were retrospectively collected, and further divided into three subgroups, namelymild, moderate, and severe hyperbilirubinemia. 127 healthy newborns with matching maternal and infant blood groups were recruited as a healthy control group. 41 cases of non-hemolytic jaundice were set up as the control group. A triple hemolytic test clarified the diagnosis of ABO-HDN, and all samples were tested for blood type and irregular antibodies. Plasma VCAM-1 were determined by enzyme-linked immunosorbent assay. Results 1 There was no statistically significant difference between the ABO-HDN group and the healthy control group regarding the sex of the newborns, birth weight, blood type, mode of delivery of their mothers, and the presence or absence of preterm rupture of membranes (P>0.05), and the neonatal gestational age, maternal age, and number of pregnancies showed significant differences between the two groups (P<0.05). There were differences in hemoglobin (Hb), reticulocyte (Ret), indirect bilirubin (IBIL), lactate dehydrogenase (LDH), high-sensitivity C-reactive protein (hs-CRP) and plasma VCAM-1 (P<0.05, and the levels of VCAM-1 were positively correlated with the levels of LDH, IBIL, and Ret, and negatively correlated with the levels of Hb (P<0.05). VCAM-1 levels in the ABO-HBN group showed independent correlations with Hb, Ret, IBIL, and LDH levels (P<0.05). Among the three subgroups, VCAM-1 levels were significantly higher in the severe hyperbilirubinemia group than in the mild and moderate hyperbilirubinemia groups (P<0.05). Conclusion Elevated VCAM-1 in children with ABO-HDN may be associated with vascular endothelial damage and with help in assessing the severity of hemolysis in the disease.

Key words: ABO hemolytic disease of the newborn, Vascular endothelial injury, Vascular cell adhesion molecule 1

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