Abstract: Objective To Analyze the change of Syphilis antibodies in patients with hematopathy before or after use of human Immunoglobulintreatment,and provide a reference for clinical diagnosis. Method Fifty-two cases of Syphilis antibody absorbance signal of sample cutoff value（S/CO）>0.5 in patients with hematological diseases were detected by chemiluminescence immunoassay （CLIA）,42 of them were injected with human immunoglobulin. Two ELISA reagents were used for verication. Syphilis haemagglutination assay（TPHA） was performed for confirmation. Nine cases were followed up for detection of Syphilis antibody after withdraw of human Immunoglobulin administration. To compare the Results of human Immunoglobulin in vivo and in vitro,times dilution the Harbin Paisi ficco Human Immunoglobulin with saline and AB type plasma to detect Syphilis antibody,simultaneous test the Results of Chengdu rongsen Human Immunoglobulin（PH4）. Result 10 patients showed positive TPHA,42 patients who had received human Immunoglobulin injection presented positive rates of 78.6%,88.1% and 66.7% in Syphilis antibody detection when tested by different reagents purchased from Johnson,Wan Tai and Sorin,TPHA,however,was found to be negative in all patients. Nine patients revealed positive Syphilis antibody after withdraw of human Immunoglobulin. Conclusion Human Immunoglobulin administration may cause a false positive detection of Syphilis antibody in patients with hematological diseases. The patients with the experience of human Immunoglobulin treatment together with low Syphilis antibody reaction should be re-checked for Syphilis antibody after three months.

Key words: Human immunoglobulin, Syphilis antibody, Hematopathy