PRP Releasate Prepared with Calcium Gluconate and Calcium Chloride in Promotion of Migration and Adhesion of UcMScs

doi:10.3969/j.issn.1671-2587.2020.01.007

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2020, Vol. 22 ›› Issue (1): 28-31.DOI: 10.3969/j.issn.1671-2587.2020.01.007

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PRP Releasate Prepared with Calcium Gluconate and Calcium Chloride in Promotion of Migration and Adhesion of UcMScs

LIU Su-rui, YANG Xiao-ya, GAO Yu-hua, et al

The 980th Hospital of Joint Logistic Support Force,Shijiazhuang 050082

Received:2018-07-11 Online:2020-02-20 Published:2020-02-28

Abstract

Abstract: Objective To observe the role of PRP releasate prepared with calcium gluconate and calcium chloride in promotion of migration and adhesion of human umbilical cord mesenchymal stromal cells (h-ucMSCs). Methods Blood cell separator was used to collect PRP releasate, which was subsequently subjected to the preparation with 10% calcium gluconate or 5% calcium chloride. The h-ucMSCs cultured with FBS was taken as the control. The migration and adhesion of the cells in the three treatments were examined by transwell or matrigel. Western blotting was used to check the expression of Cdc42, RhoA and Rac1. Results The PRP releasate prepared with both calcium gluconate and calcium chloride notably promoted the migration and adhesion of h-ucMSCs compared to the control. Meanwhile, expression of Cdc42, RhoA and Rac1 associated with cell migration and adhesion were elevated. Conclusion PRP releasate prepared with calcium gluconate may improve the migration of h-ucMSCs compared with calcium chloride, suggesting that combined use of both calcium agents with h-ucMSCs might provide a therapeutic strategy for tissue repairs.

Key words: PRP releasate, ucMSCs, Migration, Adhesion, Rho GTPases

CLC Number:

R331.1+4

LIU Su-rui, YANG Xiao-ya, GAO Yu-hua, et al. PRP Releasate Prepared with Calcium Gluconate and Calcium Chloride in Promotion of Migration and Adhesion of UcMScs[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2020, 22(1): 28-31.

References

1 Dohan Ehrenfest D M,Andia I,Zumstein M A,et al. Classification of platelet concentrates (Platelet-Rich Plasma-PRP,Platelet-rich Fibrin-PRF) for topical and infiltrative use in orthopedic and sports medicine:current consensus,clinical implications and perspectives[J]. Muscles Ligaments Tendons J,2014,4(1):3-9.
2 Harke H,Tanger D,Fürst-Denzer S,et al.Effect of a preoperative separation of platelets on the postoperative blood loss subsequent to extracorporeal circulation in open heart surgery (author's transl)[J]. Anaesthesist,1977,26(2):64-71.
3 Glenister K M,Payne K A,Sparrow R L.Proteomic analysis of supernatant from pooled buffy-coat platelet concentrates throughout 7-day storage[J]. Transfusion,2008,48(1):99-107.
4 DeLong J M,Russell R P,Mazzocca A D. Platelet-rich plasma:the PAW classification system[J]. Arthroscopy,2012,28(7):998-1009.
5 Hildner F,Eder M J,Hofer K,et al.Human platelet lysate successfully promotes proliferation and subsequent chondrogenic differentiation of adipose-derived stem cells:a comparison with articular chondrocytes[J]. J Tissue Eng Regen Med,2015,9(7):808-818.
6 Jonsdottir-Buch S M,Lieder R,Sigurjonsson O E. Platelet lysates produced from expired platelet concentrates support growth and osteogenic differentiation of mesenchymal stem cells[J]. PLoS One,2013,8(7):e68984.
7 Moreira Teixeira L S,Leijten J C,Wennink J W,et al. The effect of platelet lysate supplementation of a dextran-based hydrogel on cartilage formation. Biomaterials[J]. 2012,33(14):3651-3661.
8 Murphy M B,Blashki D,Buchanan R M,et al.Adult and umbilical cord blood-derived platelet-rich plasma for mesenchymal stem cell proliferation,chemotaxis,and cryo-preservation[J]. Biomaterials,2012,33(21):5308-5316.
9 Van Pham P,Bui K H,Ngo D Q,et al.Activated platelet-rich plasma improves adipose-derived stem cell transplantation efficiency in injured articular cartilage[J]. Stem Cell Res Ther,2013,4(4):91.
10 Cho H S,Song I H,Park S Y,et al.Individual variation in growth factor concentrations in platelet-rich plasma and its influence on human mesenchymal stem cells[J]. Korean J Lab Med,2011,31(3):212-218.
11 Huang Q,Wang Y D,Wu T,et al.Preliminary separation of the growth factors in platelet-rich plasma:effects on the proliferation of human marrow-derived mesenchymal stem cells[J]. Chin Med J (Engl),2009,122(1):83-87.
12 单桂秋,李艳辉,张雅妮,等. 血小板凝胶制备方法的体外实验研究[J].中国输血杂志,2011,24(4):270-274.
13 Qi Y,Niu L,Zhao T.Combining mesenchymal stem cell sheets with platelet-rich plasma gel/calcium phosphate particles:a novel strategy to promote bone regeneration[J]. Stem Cell Res Ther,2015,6:256.
14 Assirelli E,Filardo G,Mariani E,et al.Effect of two different preparations of platelet-rich plasma on synoviocytes[J]. Knee Surg Sports Traumatol Arthrosc,2015,23(9):2690-2703.
15 Raeissadat S A,Babaee M,Rayegani S M,et al. An overview of platelet products (PRP,PRGF,PRF,etc.) in the Iranian studies[J]. Future Sci OA,2017,3(4):FSO231.
16 Yin W J,Xu H T,Sheng J G,et al.Advantages of Pure Platelet-Rich Plasma Compared with Leukocyte- and Platelet-Rich Plasma in Treating Rabbit Knee Osteoarthritis[J]. Med Sci Monit,2016,22:1280-1290.
17 Campard D,Lysy P A,Najimi M,et al.Native umbilical cord matrix stem cells express hepatic markers and differentiate into hepatocyte-like cells[J]. Gastroenterology,2008,134(3):833-848.
18 Pereira W C,Khushnooma I,Madkaikar M,et al.Reproducible methodology for the isolation of mesenchymal stem cells from human umbilical cord and its potential for cardiomyocyte generation[J]. J Tissue Eng Regen Med,2008,2(7):394-399.
19 Ren G W,Chen X D,Dong F P,et al.Concise review:mesenchymal stem cells and translational medicine:emerging issues[J]. Stem Cell,Transl Med,2012,1(1):51-58.
20 Fu Y S,Cheng Y C,Lin M Y,et al.Conversion of human umbilical cord mesenchymal stem cells in Wharton's jelly to dopaminergic neurons in vitro:potential therapeutic application for Parkinsonism[J]. Stem Cells,2006,24(1):115-124.
21 吕璐璐,宋永平,魏旭东,等.人脐带和骨髓源间充质干细胞生物学特征的对比研究[J].中国实验血液学杂志,2008,16(1):140-146.
22 Tapon N,Hall A.Rho,Rac and Cdc42 GTPases regulate the organization of the actin cytoskeleton[J]. Curr Opin in Cell Biol, 1997,9(1):86-92.
23 刘素蕊,李俊峡,杨晓亚,等. Cdc42参与脐带间充质干细胞向炎性因子的趋化[J]. 中国组织工程研究,2014,18(23):3616-3621.

PRP Releasate Prepared with Calcium Gluconate and Calcium Chloride in Promotion of Migration and Adhesion of UcMScs

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