Abstract: Objective To investigate the clinical significance of serum β-hCG, ICAM-1 and MMP9 in the intrauterine infection and prognosis of premature rupture of membranes. Methods 296 patients with precancerous premature rupture of membranes were collected. Blood samples were drawn at the time of prenatal fasting. After treatment, the serum levels of β-hCG, ICAM-1 and MMP9 were measured. The patients were divided into non-infected group and infected group according to the infection status of the patients. There were 184 cases of non-infected group and 112 cases of infection group. Then, Serum levels of β-hCG, ICAM-1 and MMP9 were compared between the two groups, and the prognosis of the two groups was evaluated. Results The levels of MMP9, ICAM-1 and β-hCG in the non-infected group were significantly lower than those in the infection group (P<0.05). Serum MMP9, ICAM-1 and β-hCG levels were predictive of the value of intrauterine infection, the sensitivity, specificity and accuracy of ICAM-1>MMP9>β-hCG, the positive predictive value of MMP9>ICAM-1>β-hCG, The predicted value of MMP9>β-hCG> ICAM-1, but after χ2 test, no significant statistical difference, P>0.05; The combined value of the above serum markers for the predictive value of intrauterine infection in premature rupture of membranes was compared. Compared with the combined detection of the two, there was no significant difference in sensitivity and specificity (P>0.05), but the accuracy was significantly higher (P<0.05).The time and length of hospitalization of antibiotics in patients with MMP9, ICAM-1 and β-hCG positive were significantly higher than those in MMP9, ICAM-1 and β-hCG negative patients (P<0.05). Conclusion Detection of serum β-hCG, ICAM-1 and MMP9 levels in pregnant women with premature rupture of membranes can predict the situation of intrauterine infection in pregnant women more accurately and can predict the prognosis of patients.

Key words: β-hCG, ICAM-1, MMP9, Premature rupture of membranes, Intrauterine infection