Abstract: Objective The purpose of this study was to retrospectively analyze independent risk factors for HLA and/or HPA antibodies detected in patients with platelet transfusion refractoriness（PTR）. Methods The clinical data of 910 patients with PTR and their status of HLA and/or HPA antibodies were collected. The influence of sex,age,blood group, disease,blood transfusion times,hemogram parameters and coagulation index before PTR occurrence were analyzed. Results HLA and/or HPA antibodies were more easily observed in sera from women and women with a history of pregnancy. The average age of antibody-positive patients was significantly higher than that of antibody-negative patients （51.46±18.49 year vs. 40.68±20.08 year,P<0.001）. The average number of blood transfusion in antibody-positive patients was significantly lower than that in antibody-negative patients （13.13±18.64 vs. 18.66±22.52,P=0.024）. The incidence of antibody was not correlated with the number of blood transfusion. The platelet counts （13.13±18.64×10⁹/L vs. 18.66±22.52×10⁹/L, P=0.021） and hemoglobin levels （72.41±18.64 g/L vs. 78.61±21.66 g/L,P=0.009）in antibody-positive patients were also significantly lower than those in antibody-negative patients. The independent risk factors for antibody production were over 60 years old,female,MDS,frequency of transfusions and platelet counts. Conclusion The understanding of risk factors for the formation of HLA and/or HPA antibody is helpful for clinicians to take appropriate measures to avoid PTR and improve the efficacy of platelet transfusion.

Key words: Platelet transfusion refractoriness（PTR）, Human leucocyte antigen（HLA） antibody, Human platelet antigen （HPA） antibody, Independent risk factor, Retrospective analysis