Abstract: Objective 1）To explore the serological characteristics of cells with different Rh phenotypes bound to the anti-CD47 IgG4 monoclonal antibody TJC4;2）To clarify the treatment strategy of TJC4 to eliminate the interference of blood transfusion compatibility test. Method 5 mL of venous blood from patients with EDTA-K₂ anticoagulation, non-tumor and no acute or chronic underlying diseases with Rh phenotypes of CCDee, CcDEe, CcDee, ccDEE, ccDEe, ccdee, Ccdee, and CCdee were collected, using microcolumn gel method, saline Blood transfusion compatibility tests such as DAT (direct antiglobulin test), IAT (indirect antiglobulin test), and cross-matching blood were respectively performed by the method and polybrene method to detect TJC4 in vitro sensitization and idiotype antibody ID-ab neutralization. , Immucor AHG interference removal.Results 1)All Rh phenotype cells sensitized by TJC4 showed positive reaction of DAT, IAT and cross-matching with varying intensity of agglutination. The interference can be eliminated by using the Immucor AHG LISS method. 2)In order to remove the interference of CD47 McAb to ccDEe and ccdee phenotypes, higher doses should be used in idiotypic antibody ID-ab neutralization test.Conclusion 1)In this study, various blood transfusion compatibility test methods with different Rh phenotypes combined with TJC4 were used and showed notable serological differences. 2)The idiotypic antibody ID-ab neutralization test and the Immucor AHG LISS enhancer assay lacking IgG4 subtype detection can eliminate the serological interference caused by CD47 McAb. However, there were dose differences among the different Rh phenotypes.

Key words: Rh phenotype, TJC4, Serological characteristics