Abstract: Objective We aimed to establish a relatively complete operation procedure for platelet storage using classical SSP+ platelet additive solution, and to compare the quality of preserved platelet concentrates in SSP+ versus in plasma. Methods Eighty-four blood （400 mL/person） were randomly collected from the general population in Chongqing. A buffy coats product derived from the whole blood donations of 6 same blood type donors were pooled to make a buffy coats platelet concentrate（n=14）. Each pool was divided into two groups, suspended in SSP+ and plasma, respectively. Platelet count, pH, MPV, platelet metabolism, hypotonic shock response（HSR）, CD62p expression and apoptosis indicator （AnnexinⅤ） were measured on Days 1, 5 and 7. Results There were no significant differences in platelet concentration, content, volume, MPV and pO₂ during 7 days of storage between SSP+ group and plasma group （P>0.05）. However, the pH value in SSP+ group was significantly higher than that in plasma group （P<0.05）. The glucose consumption in SSP+ PAS group and the corresponding increase of lactic acid was significantly lower than that in plasma group （P<0.05）. With the decrease of pCO₂, CO₂ emission increased. The consumption of bicarbonate in additive SSP+ group was significantly lower than that in plasma group （P<0.05）. The HSR in SSP+ group was higher than that in plasma group. Both the CD62p expression and AnnexinⅤ were lower than that in plasma group. All products were performed the test for sterility on the 7th day of storage, and the results were negative. On the 7th day of storage, the pH and MPV in SSP+ group were still remained 6.94 ±0.11 and 9.89±0.52, respectively. Conclusion Platelets concentrates suspended in the classical SSP+ could metabolize normally within 7 days of storage. It has better in vitro parameters in platelet metabolism, platelet activation and apoptosis.

Key words: Platelet additive solution, SSP+, Platelet storage