Abstract: Objective To observe the effect of Daratumomab on detection of IgG anti-D antibody by polybrene test. Methods Ten plasma samples with AB RhD positive blood type and negative irregular antibody screening were selected, and each sample was divided into 3 groups by 2 mL in 3 tubes. Any substance was added in the antibody screening negative control group. IgG anti-D antibody was added in the antibody screening positive control group. Daretozumab (400 mg/20 mL) and IgG anti-D reagent were added in the experimental group. The irregular antibody screening test was performed by microcolumn gel card test and polybrene test to identify IgG anti-D antibody. Results There was significant difference between the experimental group (23.80±1.40) and the positive control group (18.80±1.40) by microcolumn gel card test (P<0.05). There was no significant difference between the experimental group (14.80±2.53) and the positive control group (14.80±2.53) by polybrene test (P>0.05). The lower limit of detection of IgG anti-D in the experimental group by polybrene test after dilution was the same result as that in the positive control group (1∶64). Conclusion Daretozumab has no effect on the detection of IgG anti D antibody by polybrene test, but has effect by the microcolumn gel card test.

Key words: Daratumomab, Alloantibody, Polybrene technique