Abstract: Objective To evaluate the disinfection effect of bloodmobile carriage (hereinafter referred to as "carriage") by hydrogen peroxide mist disinfection machine. Methods After using a hydrogen peroxide mist disinfection machine to disinfect the interior of the carriage, we evaluated the disinfection effect by testing the natural environmental air microbial counts inside the carriage, the Bacillus stearothermophilus (ATCC12980) tablets, as well as the number of surface colonies on the left and right sides of the carriage, near the interior wall of the driver's cabin, the handle positions, and around and in the center of the built-in refrigeration unit. Results After disinfection, the air colonies and surface colonies inside the carriage were qualified and the logarithmic value of extermination was >1.00, and the killing rate of Bacillus sphaericus (ATCC12980) was 100%, only the bacteria were detected in the inner door handle and the built-in refrigeration unit close to the cab (front)with the median colony counts 4.21 CFU/cm² (range: 0 CFU/cm²~6.84 CFU/cm²) and 6.84 CFU/cm² (range: 0 CFU/cm²~8.42 CFU/cm²), respectively, and the rest of the detected locations were free of bacterial growth. The median manual operation time of hydrogen peroxide mist disinfection machine was 45 s (range: 40 s~51 s), and the median of manual operation time required by conventional disinfection spray was 610 s (range: 605 s~613 s). Conclusion The use of a hydrogen peroxide mist disinfection machine can meet the disinfection requirements for air colonies and the surface of objects inside the carriage, improve work efficiency and increase safety.

Key words: Hydrogen peroxide mist disinfection machine, Bloodmobile, Logarithmic value of extermination