Abstract: Objective This study aimed to establish a visualized tumor-bearing mouse model carrying ovalbumin (OVA) and evaluate the anti-tumor efficacy of dendritic cell (DC) vaccines. Methods A lentiviral vector expressing OVA and firefly luciferase (FLUC) genes was constructed via full-length gene synthesis and transduced into B16F10 melanoma cells to generate a stable tumor cell line. The established cells were intraperitoneally injected into C57BL/6J mice to develop an omental transplantation tumor model, with tumor progression monitored using small-animal in vivo imaging. Results Tumors predominantly formed metastatic foci in the omentum, and the number of inoculated tumor cells was significantly correlated with survival duration. OVA-specific DC vaccines were further prepared, revealing that mature DC vaccines markedly suppressed tumor growth, with complete tumor regression observed in some mice, whereas immature DC vaccines exhibited limited efficacy. Conclusion This study successfully established a visualized and quantifiable intraperitoneal omental transplantation tumor model in mice, demonstrating its utility for evaluating the anti-tumor effects of DC vaccines. This model provides a valuable platform for immunotherapy research.

Key words: Omental implantation tumor, Visualized tumor monitoring, DC therapy, Anti-tumor