Evaluating the Quality and Storage Duration of Washed Red Blood Cells Resuspended in Mannitol-adenine-phosphate (MAP) Solution

doi:10.3969/j.issn.1671-2587.2025.03.016

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2025, Vol. 27 ›› Issue (3): 378-383.DOI: 10.3969/j.issn.1671-2587.2025.03.016

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Evaluating the Quality and Storage Duration of Washed Red Blood Cells Resuspended in Mannitol-adenine-phosphate (MAP) Solution

ZHAO Wei¹, ZHENG Ziwei², LU Faqiang¹, YANG Yicheng²

¹Affiliated Zhongshan Hospital of Dalian University;
²Dalian Blood Center 116001

Received:2024-12-30 Published:2025-06-23

Abstract

Abstract: Objective To compare membrane proteins dynamics and pH changes between MAP-suspended washed RBCs and suspended RBCs to determine the optimal storage period for MAP-suspended washed RBCs. Methods Packed RBCs were equally divided into two groups: suspended RBCs and MAP-suspended washed RBCs. Membrane protein expression (CD47, CD55, CD59, CD147) and PS externalization rate, along with pH values, were measured on storage days 2, 7, 14, 21, 28, and 35. Results The pH values of MAP-suspended washed RBCs were significantly lower than those of suspended RBCs at all timepoints (P<0.05). By day 35, MAP-suspended washed RBCs showed significantly reduced CD47, CD55, and CD59 expression (P<0.01), elevated PS externalization (P<0.01), and a non-significant decline in CD147. A significant correlation was observed between membrane protein changes and pH values (P<0.01). Conclusion The quality of MAP-suspended washed RBCs not inferior to that of suspended RBCs during short-term storage (≤21 days). It is recommended that the optimal storage period for MAP resuspended washed RBCs be 21 days to ensure the safety and efficacy of transfusion.

Key words: Washed RBCs, Storage duration, Quality evaluation, Membrane protein

CLC Number:

R331.1⁺41R457

ZHAO Wei, ZHENG Ziwei, LU Faqiang, YANG Yicheng. Evaluating the Quality and Storage Duration of Washed Red Blood Cells Resuspended in Mannitol-adenine-phosphate (MAP) Solution[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2025, 27(3): 378-383.

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Evaluating the Quality and Storage Duration of Washed Red Blood Cells Resuspended in Mannitol-adenine-phosphate (MAP) Solution

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