Enhancing the Prevention of Cold Storage-induced Lesions: An Experimental Investigation into Modulation of Platelet Reactive Oxygen Species via Low-level Near-infrared Laser Irradiation

doi:10.3969/j.issn.1671-2587.2024.05.007

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2024, Vol. 26 ›› Issue (5): 617-623.DOI: 10.3969/j.issn.1671-2587.2024.05.007

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Enhancing the Prevention of Cold Storage-induced Lesions: An Experimental Investigation into Modulation of Platelet Reactive Oxygen Species via Low-level Near-infrared Laser Irradiation

XIE Rufeng, ZHANG Jingyi, YANG Yiming, GAO Li, JIANG Xueyu, SUN Juan, YANG Jie

Blood Engineering Laboratory, Shanghai Blood Center, Shanghai 200051

Received:2024-06-07 Online:2024-10-20 Published:2024-09-20

Abstract

Abstract: Objective To explore the modulating effects of near-infrared low level laser (LLL) irradiation on reactive oxygen species (ROS) levels in platelets during cold storage, ultimately reducing cold storage-induced lesions and protecting the platelets from phagocytosis by macrophages and hepatocytes. Methods Buffer-coat-derived platelets were divided into three groups: room-temperature-stored platelets (RTP), cold-stored platelets (CSP), and CSP subjected to LLL irradiation (CSP-L). In the CSP-L group, platelets were irradiated with varying intensities of LLL irradiation (1 J, 5 J, 10 J) after 12 hours of cold-storage and then maintained under cold condition. After 5-day storage, ROS generation, expression of activation markers, exposure of glycosyl proteins and their residues in platelets from each group were analyzed and compared by flow cytometry (FACS). PMA-induced THP-1 cells and HepG2 hepatocytes were used to examine the phagocytosis of platelets in each group. Results After 5 days of storage, the platelet cytoplasmic ROS (cyto-ROS) level increased significantly in the CSP group compared with the RTP group (RTP vs.CSP, 3 960±259.6 vs. 5 846±757.4, P<0.01), whereas the mitochondrial ROS (mito-ROS) level was lower than that in the RTP group (RTP vs.CSP, 595±47.1 vs. 416±50.2, P<0.05). Notably, low-intensity LLL (1 J) irradiation in the CSP-L1 group significantly reduced cyto-ROS levels (5 846±757.4 vs. 3 945±459.4, P<0.01) and diminished the exposure of CD62P, phosphatidylserine (PS) expression and GPIb/ɑ glycoprotein residues compared with the CSP group (P<0.05), aligning closely with the RTP group. Furthermore, the phagocytic rate of platelets by THP-1 and HepG2 cells was also obviously lower in the CSP-L1 group than in the CSP group (P<0.05). Importantly, LLL irradiation had no significant effect on the platelet count, pH, or mean platelet volume (MPV) in cold storage. Conclusion Low-intensity LLL irradiation treatment could effectively lower the cyto-ROS levels of cold-stored platelets while maintaining low mito-ROS generation, and inhibit platelet activation and GPⅠb/ɑ desialylation, thereby reducing cold storage-induced lesions and preventing phagocytosis.

Key words: Near-infrared low level laser, Cold-stored platelet, Cold storage-induced lesions, Reactive oxygen species

CLC Number:

R457

XIE Rufeng, ZHANG Jingyi, YANG Yiming, GAO Li, JIANG Xueyu, SUN Juan, YANG Jie. Enhancing the Prevention of Cold Storage-induced Lesions: An Experimental Investigation into Modulation of Platelet Reactive Oxygen Species via Low-level Near-infrared Laser Irradiation[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2024, 26(5): 617-623.

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Enhancing the Prevention of Cold Storage-induced Lesions: An Experimental Investigation into Modulation of Platelet Reactive Oxygen Species via Low-level Near-infrared Laser Irradiation

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