The Application of Third-generation Sequencing Technology in the Study of Blood Group Gene Polymorphism

doi:10.3969/j.issn.1671-2587.2025.05.018

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2025, Vol. 27 ›› Issue (5): 701-711.DOI: 10.3969/j.issn.1671-2587.2025.05.018

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The Application of Third-generation Sequencing Technology in the Study of Blood Group Gene Polymorphism

LIANG Lixin¹, LIANG Jieling¹, WEI Yaming^2,3,4, YUAN Zhaohu^2,3,4

¹Department of Blood Transfusion Zhaoqing First People's Hospital, Zhaoqing, Guangdong 526000;
²Department of Blood Transfusion Guangzhou First People's Hospital, Guangdong 510180;
³Guangdong Engineering Research Center for Precision Blood Transfusion Technology, Guangdong 510180;
⁴Institute of Blood Transfusion and Hematology, Guangzhou Medical University, Guangzhou, Guangdong 510180

Received:2025-09-05 Revised:2025-09-12 Online:2025-10-20 Published:2025-10-11

Abstract

Abstract: Blood group gene polymorphism holds significant importance in the fields of genetic research, clinical blood transfusion, and transplant immunology, as it determines the polymorphism of blood group antigens. In recent years, third-generation sequencing (TGS) technology, also known as single-molecule sequencing, has emerged as a research hotspot in the precision medicine genetic testing industry, opening a new door for the entire field of genetics. Research findings on blood group genes based on TGS technology have been successively published, continuously expanding the application boundaries of this technology in blood group polymorphism analysis. This review summarizes the latest advancements in TGS technology for studying blood group gene polymorphism and its applications in clinical transfusion medicine.

Key words: Third-generation sequencing, Blood group genes, Single molecule real-time, Oxford nanopore sequencing

CLC Number:

R394.3

LIANG Lixin, LIANG Jieling, WEI Yaming, YUAN Zhaohu. The Application of Third-generation Sequencing Technology in the Study of Blood Group Gene Polymorphism[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2025, 27(5): 701-711.

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The Application of Third-generation Sequencing Technology in the Study of Blood Group Gene Polymorphism

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