Abstract: Objective DIA proteomics technique was used to analyze the effect of Potentilla anserina capsule on the differential expression of serum protein in rats with hemorrhagic shock under hypothermia and hypoxia. Methods Twenty-two male SD rats were randomly divided into sham operation group (n=8), model group (n=8), and treatment group (n=6). The rats in the 3 groups were fed adaptively for 1 week. Before modeling, the rats in the treatment group were given Potentilla anserina capsule daily by intragastric administration, and rats in sham operation group and model group were given the same amount of saline for 7 days. Serum samples in the sham operation group were collected during the same time after the successful modeling of the other two groups. All blood samples were subjected to proteomics to identify proteins with significant changes in expression levels, and Gene Ontology (GO) enrichment analysis was performed to determine their biological functions. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was also conducted to explore the biological pathways involved. Results A total of 3 502 protein spots were identified. Compared with sham operation, 196 proteins were significantly differentially expressed in the model group, including 110 up-regulated proteins and 86 down-regulated proteins. Compared with the model group, 104 proteins were significantly differentially expressed in the treatment group, including 68 up-regulated proteins and 36 down-regulated proteins. The protein expressions of Slfn2, Tex52, Fmod, Fabp5 and Exoc3 were significantly different between the model group and the treatment group. The GO analysis of differentially expressed proteins showed that differential proteins were mainly involve cellular processes, biological regulation, stress response, protein-containing complexes, catalytic activity, and regulation of molecular functional activity, etc. KEGG pathway analysis involves pathways such as ribosome, platelet activation, inositol phosphate metabolism, and phosphatidylinositol signaling system. Conclusion The study has confirmed that Potentilla anserina capsules can significantly change the expression of immune response-related proteins and inflammatory related proteins in serum of rats with hemorrhagic shock under hypothermia and hypoxia, which may be regulated through metabolic pathways such as ribosome metabolism and platelet activation.

Key words: Hemorrhagic shock, Potentilla anserina capsule, Proteomics