Abstract: Objective This study aimed to investigate the effects of platelet-rich plasma (PRP) on the proliferation and migration of immortalized human endometrial stromal cells (iehESCs), to determine the optimal concentration of PRP, and to identify the key gene mediating PRP-induced enhancement of cell proliferation and migration. Methods iehESCs were exposed to graded PRP concentrations. Cell proliferation and migratory capacity were assessed by CCK-8 assay and Transwell migration assay, respectively. Transcriptome profiling was performed to screen differentially expressed genes (DEGs), and the findings were validated by quantitative RT-qPCR and Western blot. Results PRP at 5% and 10% significantly enhanced both proliferation and migration of iehESCs, whereas 15% PRP showed no stimulatory effect. Transcriptomic analysis revealed 1 032 DEGs between the 5% PRP group and control group. GO and KEGG enrichment analyses highlighted 12 up-regulated and 3 down-regulated genes. Among them, CDCA8 (cell division cycle associated 8) was markedly up-regulated at both the mRNA and protein levels. Conclusion Low concentrations of PRP (5%~10%) promote iehESC proliferation and migration, whereas high concentrations (15%) are ineffective. The beneficial effects of PRP are, at least partially, mediated by up-regulation of CDCA8, which facilitates cell-cycle progression.

Key words: Platelet-rich plasma (PRP), Human endometrial stromal cells, Cell proliferation, Cell migration CDCA8