Application of Chemiluminescence Resonance Energy Transfer Technology in Functional Monitoring of Apheresis Platelets during Storage

doi:10.3969/j.issn.1671-2587.2026.04.025

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2026, Vol. 28 ›› Issue (4): 628-636.DOI: 10.3969/j.issn.1671-2587.2026.04.025

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Application of Chemiluminescence Resonance Energy Transfer Technology in Functional Monitoring of Apheresis Platelets during Storage

WANG Tao¹, WANG Xiaoqing¹, MA Qiaoqin¹, HE Linyan²

¹Changzhou Central Blood Station; Changzhou, Jiangsu, 213012;
²Jiangsu Institute of Hematology, NHC Key Laboratory of Thrombosis and Hemostasis, National Clinical Research Center for Hematologic Diseases, Collaborative Innovation Center of Hematology, Suzhou, Jiangsu 215006

Received:2026-07-06 Online:2026-08-20 Published:2026-08-26

Abstract

Abstract: Objective To investigate the application value of chemiluminescence resonance energy transfer (CRET) technology in functional monitoring of apheresis platelets during storage, and to evaluate its feasibility for detecting platelet membrane molecules and activation-related indicators in combination with flow cytometry. Methods Apheresis platelet samples were randomly collected from 48 donors in 2026 and randomly assigned to seven storage groups according to storage duration from day 1 to day 7. Flow cytometry was used to detect CD61 expression percentage, CD61 mean fluorescence intensity (MFI), CD62P expression percentage and CD62P MFI in each group. CRET technology was used to detect CD61, basal CD62P and ADP-stimulated CD62P-related indicators, and positive expression percentage and relative light units (RLU) were recorded. Intra-assay precision of the CRET method was evaluated. Results Flow cytometric CD62P expression percentage and CD62P MFI increased with prolonged storage time (P<0.05). Basal CRET-CD62P expression percentage was significantly positively correlated with storage duration (P<0.05), and basal CRET-CD62P RLU was also significantly positively correlated with storage duration (P<0.05). After ADP stimulation, both CRET-CD62P expression percentage and RLU were significantly higher than those in the basal state (P<0.05), whereas the response reserve expressed as the difference between ADP-stimulated and basal values showed no significant correlation with storage duration (P>0.05). CRET-CD61 RLU was significantly positively correlated with flow cytometric CD61 MFI (P<0.05), while the correlation between CD61 expression percentages detected by the two methods was insufficient. Repeatability testing showed that the coefficients of variation (CVs) of low- and high-level CD61 samples were 6.25% and 3.91%, respectively, and the CVs of low- and high-level CD62P samples were 14.27% and 11.43%, respectively. Conclusion CRET technology can reflect changes in basal CD62P-related activation signals during apheresis platelet storage and can detect ADP-induced platelet activation responses. It has potential value for rapid monitoring of platelet storage quality.

Key words: Apheresis platelet, Resonance energy transfer, CD62P, CD61, Platelet activation, Flow cytometry

CLC Number:

R457

WANG Tao, WANG Xiaoqing, MA Qiaoqin, HE Linyan. Application of Chemiluminescence Resonance Energy Transfer Technology in Functional Monitoring of Apheresis Platelets during Storage[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2026, 28(4): 628-636.

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Application of Chemiluminescence Resonance Energy Transfer Technology in Functional Monitoring of Apheresis Platelets during Storage

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