Abstract: Objective To explore the significance of （1,3）-β-D-glucan,galactomannan antigen and （1,3）-β-D-glucan Combined with galactomannan antigen tests in the diagnosis of invasive fungal diseases. Methods Patients suspected with invasive fungal infections were retrospectively analyzed from July 2014 to July 2016. According to the Diagnostic Criteria for Invasive Fungal Infections 2008 by US National Institute of Allergy and Infectious Fungal Disease,the patients were grouped into IFI group and non-IFI group. The sensitivity,specificity,positive and negative predictive values were analyzed by G-test,GM-test,and combined tests of both for IFI diagnosis. Results Among the 250 patients,10 patients were definitely diagnosed as IFI,100 patients were clinically diagnosed,32 patients were suspected,and fungal infection was excluded in 108 patients. The cut-off value of positive G-test was >100 pg/mL. The sensitivity,specificity,positive or negative predictive value and the Younger Index of the G-test were 74.5%,65.7%,63.6%,76.6%,and 0.402 respectively. When the cut-off value≥0.5 was taken as positive in GM-test,the above indexes were 50%,83.5%,70.5%,and 68%,respectively for the IFI diagnosis while when G-test combined with GM-test was used,the above indexes were increased. The area under ROC curve of GM-test was 0.666 whereas that of G-test was 0.712 if ≥100 pg/mL was used as positive threshold in G-test and≥0.5 in GM-test. Conclusion Both GM- and G-tests are reliable and combination of the two tests may improve the diagnosis of invasive fungal infections.

Key words: Serum galactomannan, （1,3）-β-D-glucan, GM test, G test, Invasive fungal disease