Abstract: Objective To compare the time duration of blood centrifugation and its impact on the quality of red blood cells in leukocytes-reduced suspension. Methods Sixty samples with leukocytes reduced from 400 mL whole blood were randomly divided into 3 groups, centrifuged at 4 669 gx 4℃ for 10 min, 15 min and 20 min, respectively. Hemoglobin concentration, HCT and leukocyte residues were detected and storage terminal hemolysis rate was analyzed. Results No difference was seen in all of the indicators observed with different durations of centrifugation stated above (P>0.05). Conclusion Duration of blood centrifugation for removing leukocytes has no distinct influence on the quality of red blood cells.

Key words: Centrifugation, Red blood cells, Leukocytes reduced blood