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JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2018, Vol. 20 ›› Issue (6): 643-646.DOI: 10.3969/j.issn.1671-2587.2018.06.028

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Application of Capillary Ultracentrifugation in Identification of Rh Blood Group in the Patients after Transfusion

LIU Li-na, YIN Wen, HU Xing-bin   

  1. Department of Transfusion,Xijing Hospital,No.4 Military Medical University,Xi'an,710032
  • Received:2018-02-19 Online:2018-12-20 Published:2018-11-12

Abstract: Objective To investigate the feasibility of capillary ultracentrifugation for identification of Rh blood group in patients after transfusion. Methods Erythrocytes of 10 patients with positive antibody screening and Rh antigen confirmed in the mixed field of view were washed to prepare pressed red blood cells. The blood cells were isolated by capillary ultracentrifugation. The Rh blood group was identified using both proximal and distal red blood cells. Results Ten specimens from the patients after capillary centrifugal separationwere accurately identified as Rh blood group,with its capability of identifying antibody types. Conclusion Capillary ultracentrifugation technique can isolate the red blood cells of patients following transfusion and correctly indicate the blood type of patients,being applicable to clinical blood transfusion.

Key words: Capillary ultracentrifugation, Transfusion, Rh blood group identification

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