Abstract: Objective To set up a method for detection of anti-G for Rh blood grouping and to analyze the anti-G in the Rh negative individuals with anti-D and anti-C. Methods The Rh D was tested by the gel column method. The Rh D（-）was confirmed by indirect anti-globulin tests with three different antibodies. The irregular antibodies in the sera of patients were screened and identified by cell screening panel. The adsorption-elution studies were performed to examine the specificity of the antibodies. Results The blood group of the patients was AB Rh D（-）（ccdee）.The serum detections showed agglutination reactions with AB Rh D（+）red blood cells （RBCs）. The reactivity was also seen with AB Rh D（-）RBCs with C antigen. The antibodies were demonstrated to be anti-D and anti-G after differential adsorption-elution experiments. Conclusion The anti-G is associated with hemolytic diseases such as transfusion reactions of newborns. The adsorption-elution assay is simple and practical,particularly for identification of anti-G if the specific RBCs are unavailable.

Key words: Rh negative, Irregular antibody, Anti-G, Anti-D