Abstract: Objective To explore the clinical significance of irregular antibody screening of pregnant women in diagnosis of hemolytic disease of newborns.Methods Antibody screening was performed in 3719 pregnant women by microcolumn gel anti-human globulin card method,and antibody specific identification was carried out in those with positive antibodies. Blood type serological examination and antibody specific identification of neonatal hemolytic disease were simultaneously performed.Results Twenty four cases （0.64%） of irregular antibodies were found,including 13 cases of anti-D,3 cases of anti-M,2 cases of anti-E,1 case of anti-Ce,1 case of anti-DC,1 case of anti-Lea and 3 cases of cold autoantibodies. Among the 24 pregnant women with positive antibody screening,pathological jaundice occurred and 11 cases （45.8%） were diagnosed as neonatal hemolytic disease （HDN） by serology of blood grouping.Conclusion Screening of maternal irregular antibodies is of great significance in diagnosis,prevention and treatment of neonatal hemolytic disease.

Key words: Pregnant women, Irregular antibody, Neonatal hemolytic disease