Abstract: Objective To the cause of non ABO hemolytic disease of the newborn,irregular antibodies were analysis and research,and to explore its clinical significance. Experimental sample detection method in 2010 and 2015 in hemolytic disease of the newborn,the gel cassette anti ball protein in irregular antibodies screening,Liss-IAT tube method for the identification of irregular antibody; types of irregular antibodies and related clinical data analysis. The results 2311cases of hemolytic disease of the newborn detection in experimental sample,caused by irregular antibodies hemolytic disease of the newborn （HDN） 36 cases were identified,which 28 cases of irregular antibodies for irregular antibodies of Rh blood group system,3 cases as Lewis blood group System,4 cases for Kidd blood group system,1 case for Diego blood group system. In the irregular antibodies of Rh blood group system,2 cases of anti-D,7.1% （2/28）; 8 cases for anti-e,accounting for 28.6% （8/28）; 11 cases anti -cE accounted for 39.3% （11/28）; 2 cases anti-C,accounting for 7.1% （2/28）; 5 cases anti CE,accounting for 17.9% （5/28）; 36 cases of hemolytic disease of the newborn cases,26 cases of exchange transfusion in the treatment of.Conclusion the irregular antibody prone to cause severe hemolytic disease of the newborn and in detection should be the sensitive method,avoid To avoid missed and misdiagnosis.

Key words: Hemolytic disease of the newborn Irregular antibody, Transfusion therapy