Abstract: Objective To report 10 cases of N-ABO-hemolytic disease in newborns （N-ABO-HDN） caused by maternal irregular antibodies through retrospective analysis. Methods The NEO was used to detect the irregular antibodies,and the positive samples were further identified. Cord or venous blood were collected from 74 pregnant women with irregular antibodies for hemolytic disease of newborns after delivery. Results Seventy four of 23 241 cases were found to present positive irregular antibodies. Among them,10 case of the survived infants were diagnosed as N-ABO-HDN. Two cases showed anti M-HDN; 3 cases,anti D -HDN; and 5 cases,anti E （anti Ec） -HDN. Conclusion Examination of irregular antibodies in pregnant women is quite usefull in ensuring the security of both mathers and foetuses. Early detection of irregular antibodies is crucial in multiparous pregnancies,especially in those with maternal habitual abortions.

Key words: Irregular antibody, Maternal, Hemolytic Disease of Newborn（HDN）