Abstract: Objective We compare the outcomes of Hepatitis B surface antigen （HbsAg）by enzymelinked immunosorbent assay with colloidal gold method in order to provide reference for clinic. Methods The five markers of Hepatitis B in pregnant women were detected by enzymelinked immunosorbent assay,there are 60 positive serum and 100 negative serum. Those samples were detected by colloidal gold method again. Results Both methods may detect 150 same positive outcomes and 100 negative outcomes,only 10 sample was discrepancy.,Positive predictive value of colloidal gold method was 98.3%,specificity was 100%,sensitivity was 98.1%,accuracy was 98.2%. Conclusions Compared to chemiluminiscence,Colloidal gold method is rapid、convenient,can satisfy the pregnatnt need,but can not replace chemiluminiscence.

Key words: Pregnant women, Hepatitis B surface antigen