Abstract: Objective To analyze the characteristic of RhD negative blood type and anti-D antibodies and their association with hemolytic diseases of newborns (HDNs) in Nanning, Guigang and Baise regions of Guangxi province. Methods A total of 96 471 samples from pregnant women with no blood transfusion were tested with blood type cards and tube testing. Results 304 samples (0.32%) of RhD-negative blood were found in the pregnant woman and 7 of them had co-test with anti-D antibodies（2.30﹪）, among them, 5 newborns（71.43%） suffered from HDNs. Conclusion The distribution frequency of RhD negative blood type varied in regions of Guangxi, with different frequencies of positive anti-D antibodies that may be associated with hemolytic diseases of infants.

Key words: RhD negative blood type, Pregnant women, Anti-D antibody, Hemolytic disease of newborns