RHD Gene Genotyping in Pregnant Women with Rare Blood Type

doi:10.3969/j.issn.1671-2587.2019.04.004

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2019, Vol. 21 ›› Issue (4): 348-351.DOI: 10.3969/j.issn.1671-2587.2019.04.004

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RHD Gene Genotyping in Pregnant Women with Rare Blood Type

LIANG Zhi-hong, XIE Jing-wen, RUAN Li-fen, et al

City Bridge Hospital of Guangzhou Panyu Distric 511400

Received:2018-08-07 Online:2019-08-20 Published:2019-08-28

Abstract

Abstract: Objective To study the polymorphism of RHD in the pregnant women with RhD（-）who were detected by serological method so as to improve the prenatal screening and prevent hemolytic disorders of the newborns in Panyu district of Guangzhou. Methods Eighteen cases of RhD (-) pregnant women were collected. The RhD (-) recognition test was carried out with indirect anti-human globulin method, and the RhD (-) samples were verified by absorption and release test for identification of DeL phenotype. RHD genotyping was performed with PCR-SSP. Results Serological tests revealed 18 cases of RhD (-) and 4 cases of DeL phenotype (22.2%). Genotyping results showed 10 cases of 1~10 exons (55.6%), 4 cases of RHD1227A DEL (22.2%), 3 cases of RHD-CE (2-9)-D (16.7%), and one case of positive RHD. Conclusions The RHD1227A DEL variation of RHD gene dominates, suggesting that a combination of RHD genotyping with anti-D antibody detection might help guide prophylactic use of Rh immunoglobulin to prevent hemolytic diseases in newborn infants.

Key words: RHD, Blood type, Genotyping, Polymorphism, Pregnant women

CLC Number:

LIANG Zhi-hong, XIE Jing-wen, RUAN Li-fen, et al. RHD Gene Genotyping in Pregnant Women with Rare Blood Type[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2019, 21(4): 348-351.

References

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RHD Gene Genotyping in Pregnant Women with Rare Blood Type

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