The Effect of RhD Polypeptide to Inhibit the Reaction of RhD Antigen and Anti-D

doi:10.3969/j.issn.1671-2587.2020.01.006

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2020, Vol. 22 ›› Issue (1): 23-27.DOI: 10.3969/j.issn.1671-2587.2020.01.006

Previous Articles Next Articles

The Effect of RhD Polypeptide to Inhibit the Reaction of RhD Antigen and Anti-D

WU Fan, ZHUANG Nai-bao, LIANG Shuang, et al

Shenzhen Blood Center,Shenzhen 518035

Received:2018-12-07 Online:2020-02-20 Published:2020-02-28

Abstract

Abstract: Objective To evaluate the RhD polypeptide's inhibitory effect of the reaction of RhD antigen and anti-D. Methods RhD phage polypeptides were synthesized according to the amino acid sequence from phage display library screening. The physical and chemical properties and secondary structure of polypeptides were analyzed by bioinformatics. The effective polypeptides were verified by agglutination inhibition experiment using microcolumn gel (LISS and anti-human globulin). The effective blocking concentration of polypeptides was observed by using microscope to observe the agglutination reaction. The blocking effect of polypeptides was detected by flow cytometry. Results Peptide 4 is the most stable polypeptide. There are no random coil and antigenic in this polypeptide. Peptide 4 is able to inhibit the agglutination of IgG monoclonal anti-D and D+ cells in a concentration dependent manner and completely inhibit the reaction when the concentration reached 2.5 mg/50 μL. Conclusion Peptide 4 is the effective polypeptide. It is able to block the binding sites of anti-D and RhD antigen. The result has great significance to study the structure and function of RhD protein, to modify the antigen of RhD protein, and to improve the safety of clinical blood transfusion.

Key words: Rh blood group, Polypeptide, Anti-D antibody, D antigen

CLC Number:

R446.63

WU Fan, ZHUANG Nai-bao, LIANG Shuang, et al. The Effect of RhD Polypeptide to Inhibit the Reaction of RhD Antigen and Anti-D[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2020, 22(1): 23-27.

References

1 Maureen R Newman,Danielle S W Benoit. In Vivo Translation of Peptide-Targeted Drug Delivery SystemsDiscovered by Phage Display[J].Bioconjug Chem,2018,29(7):2161-2169.
2 Steven Janvier,Karlien Cheyns,Michael Canfyn,et al.Impurity profiling of the most frequently encountered falsified polypeptide drugs on the Belgian market[J].Talanta,2018,188:795-807.
3 Tecla Ciociola,Walter Magliani,Laura Giovati,et al.Antibodies as an unlimited source of anti-infective,anti-tumour and immunomodulatory peptides[J].Sci Prog,2014,97(Pt 3):215-233.
4 Huiyuan Li,Rachna Aneja,Irwin Chaiken.Click chemistry in peptide-based drug design[J].Molecules,2013,18(8):9797-9817.
5 李小飞,刘兴莉.新生儿体内高效价抗-D遮蔽RhD抗原1例[J].中国输血杂志,2012,25(8):798-799.
6 吴凡,庄乃保,张印则,等.筛选及鉴定IgG型单克隆抗-D噬菌体克隆的实验研究[J].中国输血杂志,2017,30(10):1121-1125.
7 Tom Frame,Tim Carroll,Elena Korchagina,et al.Synthetic glycolipid modification of red blood cell membranes[J]. Transfusion,2007,47(5):876-882.
8 高红伟,李素波,鲍国强,等.原核基因工程制备新型重组α-半乳糖苷酶应用于人B→O血型改造的研究[J].中国实验血液学杂志,2011,19(2):503-507.
9 Danielle M Raymond,Bradley L Nilsson.Multicomponent peptide assemblies[J]. Chem Soc Rev,2018,47(10):3659-3720.
10 Fidel Toldrá,Milagro Reig,M-Concepción Aristoy,et al.Generation of bioactive peptides during food processing[J].Food Chem,2018,267:395-404.
11 Anna Lucia Tornesello,Maria Lina Tornesello,Franco M Buonaguro.An Overview of Bioactive Peptides for in vivo Imaging and Therapy in Human Diseases[J].Mini Rev Med Chem,2017,17(9):758-770.
12 Barrios-Lopez B,Raki M,Bergström K.Radiolabeled peptides for Alzheimer's diagnostic imaging:mini review[J]. Curr Radiopharm. 2013,6(4):181-191.
13 Valentina Ambrosini,Stefano Fanti.68Ga-DOTA-peptides in the diagnosis of NET[J]. PET Clin,2014,9(1):37-42.
14 Crystal S F Cheung,Julian C Lui,Jeffrey Baron. Identification of chondrocyte-binding peptides by phage display[J].J Orthop Res,2013,31(7):1053-1058.
15 Jong Min Lim,Ji Hong Kim,Myung Yi Ryu,et al.An electrochemical peptide sensor for detection of dengue fever biomarker NS1[J].Anal Chim Acta,2018,1026:109-116.
16 Richard G Kay,Benjamin G Challis,Ruth T Casey,et al.Peptidomic analysis of endogenous plasma peptides from patients with pancreatic neuroendocrine tumours[J]. Rapid Commun Mass Spectrom,2018,32(16):1414-1424.

[1]	WANG Lei, WU Xue-zhong. Retrospective Analysis of Rh (D,C, c, E, e) Antigens Compatibility between Blood Recipients and Donors [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(2): 181-185.
[2]	WANG Wen-ting, DANG Pan-yu, ZHANG Bao-ping, et al. Transfusion Strategy and Prognosis of Hemolytic Disease of the Fetus and Newborn Caused by Anti-D Antibody [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(1): 38-40.
[3]	FAN Liang-feng, LU Qiong, JIANG Yue-qing, et al. Changes of Hemolysis Rate,Blood Type Antigens and Antibodies in Human Blood after Incubation at 56℃ [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(1): 54-60.
[4]	LU Chun-liu, SU Jian-cong, HUANG Jian, et al. A Correlative Analysis of RhD Negative Blood Type with Anti-D Antibodies and Hemolytic Diseases of Newborns (HDNs) in Guangxi District [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2019, 21(4): 345-347.
[5]	LIU Li-na, YIN Wen, HU Xing-bin. Application of Capillary Ultracentrifugation in Identification of Rh Blood Group in the Patients after Transfusion [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2018, 20(6): 643-646.
[6]	YAO Run, LING Han, LI Ning, et al. Comparison of the Blood-matching Compatibility Rate of Rh Blood Group System Antibody in Three Blood Banks [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2018, 20(6): 652-655.
[7]	WANG Min, WANG Bao-Long, ZHOU Ming, et al. The Safety of DEL Red Blood Transfusion in RhD-negative Recipients [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2017, 19(3): 230-233.
[8]	CHEN Qing, LI Ping, LU Le, et al. Identification of Weak D Type 15 of Rh Blood Group System [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2016, 18(5): 416-419.

The Effect of RhD Polypeptide to Inhibit the Reaction of RhD Antigen and Anti-D

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 8

Recommended Articles

Metrics

Comments

About

Author

Journal

Access Statistics

Contact