Abstract: Objective To understand the cross matching compatibility rate of Rh system antibody in Changsha、Zhuzhou and Yueyang blood bank. To solve the problem of clinical difficulty blood matching as soon as possible and find a matched blood timely. Method 310 cases of the distribution of Rh system antibody were reviewed. The data of cross-matching in 310 cases was analyzed. Serological method used to detect Rh antigen in blood donors. Results 310 cases of Rh antibody distribution were as follows：anti-E 166 cases （53.54%）,anti-D 76 cases （24.52%）,anti-c 27 cases （8.71%）,anti-cE 19 cases （6.13%）,anti-C 14 cases （4.52%）,anti-Ce 4 cases （1.29%）,anti-e 4 cases （1.29%）. In type A blood,the highest compatibility rates of anti-E（69.06%）,anti-c（57.55%）,and anti-cE（69.06%） were in Changsha blood bank,while anti-C（29.05%） was in Yueyang blood bank. In type B blood,the highest compatibility rate of anti-cE（50.00%） was in Yueyang blood bank. In type AB blood,the highest compatibility rate of anti-E（62.50%） was in Changsha blood bank. In type O blood,the highest compatibility rate of anti-E（56.79%） was in Changsha blood bank. Conclusion The cross matching of anti-E、anti-c and anti-cE in type A blood,anti-E in type AB blood,anti-E in type O blood was preferred in Changsha blood bank. The cross matching of anti-C in type A blood,anti-cE in type B blood was preferred in Yueyang blood bank.

Key words: Rh blood group, Cross-match, Rh antigen