Abstract: Objective Irregular blood group antibodies were detected in patients to prevent the occurrence of allogeneic delayed hemolytic transfusion reaction （DHTR） and transfusion safety. Methods Classical anti-human globulin test was taken to detect the specific antibodies in the serum of patients. Results The serum titers of IgM and IgG of anti-E were 1∶32 and 1∶128,respectively. Conclusion Rh antigen （CcEe） typing is necessary in the patients who have primary blood transfusion of red blood cells. Screening of irregular antibodies before transfusion is especially important to reduce the reverse reaction of hemolytic transfusion and ensure the clinical blood transfusion safety.

Key words: Immunity, Antibody identification, Rh antigen typing, Delayed hemolytic transfusion, Reaction