Objective To explore the effects of blood transfusion on the vital signs and hemodynamics in preterm infants with anemia. Methods A total of 60 anemic preterm infants with gestational age less than 34 weeks who accepted blood transfusion one week after birth were enrolled for the study from January 2013 to June 2015.Each infant had 2sets of vital and echocardiographic evaluations within 24 h after transfusion.The following data were collected：respiratory rate in breaths/min,heart rate in beats/min,diastolic,systolic,and mean arterial blood pressures （DBP,SBP,and MBP） in mmHg,ventilation indexes,oxygenation and lactic acid,and left ventricular output （LVO） in ml/min/kg. Results The heart rate and respiratory rate decreased significantly within 24 h after blood transfusion （P<0.05）.The systolic pressure,diastolic pressure,mean arterial pressure,and body temperature were not statistically different in pre- and post-transfusion （P>0.05）.The LVO and SV showed no significant changes after transfusion （P>0.05）. Conclusions Blood transfusion can decrease heart rate and respiratory rate and shows no significant effect on the hemodynamics in preterm infants with anemia.And no significant improvement was noted in oxygenation following blood transfusion in preterm infants.

Objective To compare the application effects of acute normovolemic hemodilution（ANH） and acute hypervolemic hemodilution （AHH） in ectopic pregnancy surgery. Methods 96 cases of surgical patients with ectopic pregnancy were randomly divided into ANH group,AHH group and control group,32 cases in each group. The patients in ANH group were received ANH after anesthesia induction and before the operation,the patients in AHH group were received AHH after anesthesia induction and before the operation,the patients in control group were not received acute hemodilution before the operation,the compound sodium lactate injection was infused after anesthesia induction and before the operation. The indicators such as Hb,Hct,BE,LAC were compared before and after acute hemodilution in ANH group and AHH group. Mean arterial pressure （MAP）,heart rate（HR）,cardiac index （CI） and stroke volume variation rate （SVV） at different time points were compared in ANH group and AHH group. The levels of Hb,Hct,PLT and cases of allogeneic blood transfusion were compared after the operation in three groups. Results ①The indicators such as Hb,Hct,BE,LAC were no statistical differences（P>0.05）before and after acute hemodilution in ANH group and AHH group. ② MAP,CI before and after autologous blood transfusion in ANH group was higher than those in AHH group（P<0.05）,and SVV was lower than that in AHH group（P<0.05）.③The levels of Hb、Hct、PLT at 2 h after the operation in ANH group were higher than those in AHH group and control group（P<0.05）,the cases and volumes of allogeneic blood transfusion in ANH group were less than those in AHH group and control group（P<0.05）. The cases and volumes of allogeneic blood transfusion in AHH group were less than those in control group（P<0.05）. Conclusion ANH can effectively reduce the loss of red blood cells in ectopic pregnancy surgery and reduce allogeneic blood transfusion,has less influence on hemodynamics,safety performance is good,has a certain clinical value.

Objective To analyze the total blood consumption for surgical and non-surgical use in hospitals at all levels in Jiyuan.Try to implement a further strengthened management on blood for clinical use. Method Divided hospital into 3 levels,including one tertiary,eight secondary,and fifteen primary.Then made statistical analysis on four following factors：the inpatients and surgery operation numbers,surgical and total consumption of blood,the blood constituent condition of surgical and non-surgical infusion as well as the blood consumption of each constituent in tertiary hospital （surgery and non-surgery）. Result The blood usage for surgery occupies 55.8% of total blood consumption of the whole city,among which the infusion rate of plasma,erythrocytes,cryoprecipitate and platelet are 49.1%,46.3%,3.1% and 1.5% respectively.The blood consumption of tertiary hospital for clinical use accounts for 57.7% of total city blood consumption,among which blood usage for surgery,emergency treatment,ICU and non-surgical use are 52.3%,4.5%,16.8% and 27.4% respectively.The blood consumption of secondary and primary hospitals occupies 40.3% and 1.9% of the whole consumption. Conclusion The infusion rate of platelet for hospitals at all levels appears to be low while secondary and primary hospitals use extra plasma for surgery.It indicates that clinicians are not familiar with varieties and properties of blood,thus management and instruction shall be enhanced to ensure scientific and reasonable blood transfusion.

Objective To analyze the changes of infectious indexes of apheresis platelet donors in Chengdu from 2012 to 2015,find ways to reduce fraction defective and determine the target recruitment donors of apheresis platelet. Methods Retrospectively analyzed the blood screening results of 60,132 apheresis platelet blood donors from 2012 to 2015 in Chengdu.The unqualified results were analyzed by χ² test. Results The unqualified rate of platelet detection in Chengdu was 3.45%.There was statistically significant difference in annual unqualified rate of the detection （χ² =61.049,P<0.05）.HBsAg and anti-TP were the major unqualified items.There were significant differences in different age groups （χ²=42.299,P<0.05）. Conclusion Test rate in the past three years showed a downward trend.We should increase the publicity efforts of voluntary blood donation,improve screening and blood testingand recruitment of low-risk blood donors.

Objective To improve the efficiency of blood resource utilization in various blood storage sites. Methods The institutions and volumes of blood supply and the expired blood scraps were analyzed in three blood storage sites one year before and after operation. Results Construction of the blood storage facilities provided the convenience for hospital users due to their easy access for emergency treatments,and markedly decreased the ratio of blood expired scraps. Conclusion Blood storagesiting and facility availability may help integrate the resources of the blood supply network to hospitals and healthcare institutions.

Objective This study was designed to evaluate the efficacy and safety of washed RBCs by WG-XDJ-1 RBC washer in the transfusion treatment. Methods A randomized double blind control trial was conducted.Individuals were randomly divided trial group （55 cases） and control group （54 cases）. The two groups were treated with washed RBCs 2 units prepared by WG-XDJ-1 RBC washer and ACP215 RBC washer respectively. Before and after blood transfusion,we recorded the patients' changes about clinical symptoms and physical signs and also examined blood routine,hepatic and renal functions.The effective rates of blood transfusion was evaluated by intention-to-treat （ITT）analysis and per-protocol（PP） analysis,using χ² test; The adverse transfusion reaction of the 2 groups were compared by χ² test;The preservation period of RBCs of the 2 groups before washing were compared by independent samples t test; Wilcoxon rank sum test was used to compare the liver and kidney function before and after blood transfusion in the experimental group. Results According to ITT analysis,the effective rates of trial and control groups were 78.94%,71.93% and there was no difference between trial and control groups （P>0.05）; According to PP analysis,the effective rates of trial and control groups were 81.81%,75.93% and there was no difference between trial and control groups （P>0.05）.The preservation period of RBCs of trial and control groups were 7.037±0.823 days and 7.204±0.877 days and there was no difference between trial and control groups （P>0.05）.The incidences of adverse transfusion reaction were 0.00% and 1.85% for trial and control groups.There was no difference between trial and control groups （P>0.05）.There was no difference in hepatic and renal functions before and after blood transfusion in the trial group（P>0.05）. Conclusion The transfusion treatment with washed RBCs prepared by WG-XDJ-1 RBC washer was effective and well tolerated.

Objective To study the correlation between the changes of RBCs morphology and injury in the process of freezing-thawing-deglycerolizing. Methods The matching design method was employed to prepare frozen RBCs and thawed deglycerolized RBCs. Method1 : without supernatant glycerol reduction before being frozen. Method2 : supernatant glycerol reduction by centrifugation before being frozen. Take samples for blood routine test and free hemoglobin (FHb) content detection in the process of freezing-thawing-deglycerolizing, analysis the shape variation of red blood cells and its correlation with injury. Result (1) In the process of freezing-thawing-deglycerolizing, volume of red blood cell increase due to glycerolize ,and then recovery due to deglycerolize. Compare with the method 2 and method 1, mean corpuscular volume (MCV) of glycerolized RBCs (before being frozen) and thawed deglycerolized RBCs increased (P<0.01, P<0.05);(2) The destruction of red blood cells is distributed in three processes, deglycerolizing process (63.20±10.99) %, glycerolizing process (30.78±10.47) %, freezing-thawing process (6.02±4.11) %, no statistical difference between two kinds of preparation methods.(3) the red blood cell damage is moderate negative correlation with volume change rate of glycerolized RBCs ; (4) with the extension of storage time, free hemoglobin content of thawed deglycerolized RBCs gradually increased. In which the 0.9% NaCl suspension thawed deglycerolized RBCs, the content of FHb showed significantly different 12 h and 24 h after preparation as well (P<0.05).In which the MAP suspension thawed deglycerolized RBCs, the content of FHb showed significantly different 21d and 28d after preparation as well (P<0.05). Conclusion In the process of freezing-thawing-deglycerolizing, red blood cell damage is negatively related to its deformation ability, and the damage mainly happens on the deglycerolizing process. The removal of supernatant glycerol by centrifugation caused the red cell membrane skeleton to be damaged by the shear stress, thereby adversely affecting the storage quality of the thawed deglycerolized RBCs.

Objective To establish a reference range of thyroid stimulating hormone（TSH） and free thyroxine（FT4） in pregnant women in Hefei. Methods A total of 1 309 women in the normal pregnancy period were collected and divided into three groupsof T1,T2 and T3 depending on their different gestation periods from June 2016 to June 2017. Additionally,150 cases of non-gestation women were selected for pre-pregnancy screening in the hospital as control. Chemiluminescence immunoassay was used for detection of the serum thyroid peroxidase antibody （TPOAb）,TSH,and FT4. Results The reference range of the serum level of TSH was 0.06-5.74mIU/L in the early stage of pregnancy,0.10-5.30mIU/L in the medium term and 0.53-4.67mIU/L in the last term,respectively.The range of FT4values in the early,medium and late periods were 8.29-15.28pmol/L,6.56-11.97pmol/L,and 5.79-10.30pmol/L,respectively. Conclusion The TSH reference range varies in regions and the distinct values should be establishedso as toevaluatethe thyroid function of pregnant women timely and precisely.

Objective To understand acute upper respiratory trace infections （acute upper respiratory tract infection,AURTI） people hospitalized children with partial pulmonary virus（ human metapneumovirus,hMPV） infection status and the prevalence of hMPV advantage subtypes in Shenzhen area. Methods From June 2015 to June 2016 in shenzhen longhua district people's hospital and Hutian people's hospital hospitalized Children with AURTI nasopharyngeal drain or swallow test specimens 1 720,at the same time respiratory specimens from 60 healthy children of the same period were collected,Qiagen One - step RT-PCR method for screening of hMPV in the sample was employed,and amplification products randomly selected from 6 cases of hMPV positive specimens were sequenced by CEQ8800 sequencer through forward and reverse primers,BLAST software was used to sequence alignment analysis for sequencing of gene sequences with GenBank representative strains . Results Nasopharyngeal hMPV positive specimens of 362 cases were detected from 1 720 cases of hospitalized children with AURTI drains content or swallow test specimen,positive rate was 21.05% （362/1 720）,the boy's positive rate was 10.06% （173/1 720）,the girl's positive rate was 10.99% （189/1 720）,there was no statistically significant difference between male and female children with positive rate （χ²=0.917,P>0.05）,and healthy children hMPV were all negative; <1 year old group AURTI hospitalized with an hMPV infection rate was 31.46%,significantly higher than the other group,and the difference was statistically significant （χ²=6.105~11.973,P<0.05~0.01）;1~3 month AURTI hospitalized children with hMPV infection positive rate was 49.74%,significantly higher than the other month,difference was statistically significant between （χ²=7.927~15.038,P<0.05~0.01）; The virus nucleotide of the six hMPV positive strains was 91.0%~100.0%. 6 strains of hMPV positive strains belong to the A2b and B1 and B2 subtype respectively. Conclusionh MPV is one of the main pathogens of infection in children with AURTI in the hospital in Shenzhen area,occurs in winter and spring seasons and children under the age of 1,A and B are two prevalent genotypes.

Objective To observe the diagnostic value of des-gamma-carboxy prothrombin （DCP）and explore effective methods for early diagnosis of hepatocellular carcinoma（HCC）. Methods Fourty cases of HCC patients were collected from 2013 to 2016 in the hospital; 40 inpatients with other liver diseases and cancers except for HCC,and 40 healthy subjects were taken as control. ELISA and electrochemiluminescence immunoassay were used to detect DCP and AFP for comparison of DCP and AFP with those in the four groups so as to reveal the relations of their concentrations to HCC clinical characters. Results DCP concentrations（ng/ml）in HCC,liver tumors and liver disorders rather than HCC,and control groups were found to be 642.7±467.8,8.96±6.71,10.62±7.71 and 3.69±2.00 respectively;AFP concentrations（ng/ml）were 781.0±683.9,8.92±7.65,9.10±8.42 and 4.16±2.83 resectively. The average concentrations of DCP and AFP in HCC group were notably higher than those in the other three groups（P<0.01）. No significant difference of the concentrations of DCP and AFP was seen between liver tumors and other liver disorders（P>0.05）. Based on the cut-off value of DCP>12 ng/ml,the sensitivity of 80.0% and specificity of 83.2% were obtained while on the value of AFP>20ng/ml，sesitivity of 75.0% and specificity of 86.0% were noted. A combined tests of DCP and AFP gave rise to the sensitivity and specificity of as high as 87.5% and 88.7% respectively. Correlation analysis showed that DCP concentration was positively related to tumor size（r=0.632，P＜0.021）. Conclusion A combined test of DCP and AFP may benefit for early diagnosis of HCC although DCP is an essential marker.

Objective To investigate the predictive value of serum fibronectin （FN） in the risk of preeclampsia in obese pregnant women. Methods From January 2016 to May 2017,120 cases for health care during pregnancy pregnant women were selected in Hebei province people's Hospital,60 cases of normal pregnant women with normal blood pressure and no pre eclampsia,including pregnant women of normal weight （A group） and obese pregnant women （B group） each 30 cases; another 60 cases of pregnant women with preeclampsia,including pregnant women of normal weight （C group） and obese pregnant women （D group）each 30 cases,the serum FN levels were detected in pregnant women of four groups,and the outcome of pregnancy in patients with preeclampsia and pre eclampsia was compared. Results Compared with A group,the BMI,MAP,serum FN level of B group increased significantly（P<0.05）;Compared with C group,the BMI of D group increased significantly （P<0.05）,but MAP,serum level of FN was no statistical significance （P>0.05）;Compared with A and B group,the serum level of FN in C and D group significantly increased,and the D group was significantly higher than that of C group （P<0.05）;Compared with pre eclampsia,the cesarean section rate,neonatal Apgar score,fetal distress,premature delivery,intrauterine growth retardation were significantly reduced in no-preeclampsia pregnant women（P<0.05）,but there were no statistical significance in gestational age of delivery and stillbirth between above two groups （P>0.05）. Conclusion The detection of serum FN level has some predictive value for the risk of pre eclampsia in pregnant women with obesity,and worse pregnancy outcome of preeclampsia.

Objective To investigate the concentration and its significance of interleukin -32 （IL-32） in sera of patients with asthma. Methods One hundred and sixty five cases of asthma were selected,including 65 cases of acute attack,42 cases of chronic asthma and 58 stable phases and 50 healthy controls. Enzyme linked immunosorbent assay was used to detect serum IL-32. The respiratory function,the number of EOS,ECP level,and ACT score were measured and the correlation between IL-32 and the serum indicators was analyzed. Results IL-32 concentrations were found to be 53.87+20.07 ng/L,43.48+7.17 ng/L,29.55+6.26 ng/L,and 13.68+4.16 ng/L in acute,chronic and stable asthma,and healthy control,respectively （P<0.05）. FEV1,FEV1/FVC and PEF increased while EOS and ECP decreased with remission of the disease. ACT score became high with amelioration. The concentration of IL32 in sera was negatively correlated with FEV1,FEV1/FVC and ACT,but positively correlated with EOS and ECP （P<0.05）. No correlation of IL32 level in the sera was noted with PEF. Conclusion IL-32 is closely related to the occurrence and advance of asthma. Monitoring concentration of IL-32 in the sera of patients may help for the diagnosis,treatment and prognosis of asthma.

Objective To avoid to send wrong test reports,we screen and ascertain suggestive parameter of cold agglutination in specimens of blood routine. Methods The specimen of cold agglutinin was tested immediate by the sysmex XE-2100,at the same time,make the physiological salt solution smear（PSSS） to observe RBC's modality under the microscope. Results Various RBC conglomeration can be found under microscope in all the specimen of cold agglutinin PSSS with MCH,MCHC prominent increased（MCH>33 pg,MCHC>380 g/L）,RBC prominent decreased in blood routine report. Conclusion MCH,MCHC prominent increased and RBC prominent decreased were the suggestive parameter in blood routine of cold agglutination specimens.

Objective To explore the value of thinprep cytology test （TCT） and human papilloma virus （HPV） test in cervical intraepithelial neoplasia （CIN） screening. Methods A retrospective analysis was performed by using the data of 674 patients with neoplasia from 2012 to 2015,and all patients received TCT and HPV tests and confirmed diagnoses were made by biopsy. Results Five hundred and seventy eight of 674 cases received TCT tests and 508 cases received HPV tests. TCT or HPV tests were positive in 509 （88.1%） and 459 cases （90.4%）,respectively. Three hundred and fifty of 444 cases （78.8%） were found to have overlaped positivity,and 90 cases were TCT positive （20.3%）,with general positive rate of 99.1%. The results showed that a combined testing gives rise to a higher positive rate than the single test（P<0.05）although no significant difference was noted between TCT and HPV（P>0.05）. Conclusion Combination tests of both TCT and HPV may improve the efficacy and avoid missed diagnosis in CIN screening.

Objective To explore the GLS1 expression in gastric cancer tissues and its relationship with clinical prognosis. Methods A total of 88 samples of gastric mucosa cancer tissues and theiradjacent tumor mucosa epithelial tissueswere collectedfrom the patients with gastric carcinoma.The GLS1,Raptor,Rictor protein expressions were detected by immunohistochemistry,and the mRNA expressionsweretested by RT-PCR.The relationship betweenclinical pathological data and GLS1 expressionwere analysed.The patients were followed up for the survival rate. Results Frequencies of positiveexpressionsof GLS1（80.7%,71/88）,Raptor（73.9%,65/88）,and Rictor（88.6%,78/88） in gastric cancer tissuesweresignificantly higher than those in the adjacent tissues of 38.6%（34/88）,23.9%（21/88）,and 46.6%（41/88）,respectively （P<0.05）.Additionally,mRNA expressionsof GLS1,Raptor,and Rictor in the cancer tissueswere （1.04±0.21）,（1.73±0.32）,and （1.32±0.17）,respectively,significantly higher （P<0.05）than those in adjacent tissues（0.65±0.09）,（0.81±0.12）,and（0.75±0.11）.Patients with tumors of bigger than 5cm in diameter,low differentiation,III~IV stage,lymph node metastasis,and the depth of invasion between T3~T4 presented a significantly high GLS1 positivity （P<0.05）.The three year cumulative survival rate of patients with negativeGLS1 was 78.1%,higher than 47.2%（P<0.05） in positiveGLS1 patients.Multivariate analysis showed that GLS1 expression,differentiation degree and TNM stage were independent risk factors for prognosis of patients with gastric cancer （P<0.05）. Conclusion The expression level of GLS1 in gastric carcinoma are significantly elevated,and the high GLS1 expression is associated with the poor prognosis of the disease,suggesting that GLS1 may be used as an indicator for evaluating the prognosis of gastric cancer.

Objective To learn the effect of paraprotein in bilirubin measured by diazo method, vanadate oxidation and dry chemistry through bilirubin concentration basing on in the patients with paraprotein. Methods The serum of 247 cases of patients with paraprotein positive from Anqing Municipal Hospital from August 2015 to June 2017 was collected and divided into treatment groups: multiple myeloma (MM) group and non MM group. The serum of 50 case of patients with negative paraprotein was collected as the control group.Each sample was analyzed by dry chemical method, diazonium method, and vanadate oxidation method.. Analyse the test results of the determination of bilirubin in ways of diazo and vanadate compared with dry chemical. Results The difference of the bilirubin concentration by the means of diazonium and vanadate oxidation were statistically significant in the group of MM and non MM,while the difference were not statistically significant in the control group.The D-value of diazo method and dry chemistry were statistically significant in the MM group and control group, as in the groups of MM and non MM,meanwhile they were statistically significant in the non MM group and control group.The D-value of vanadate oxidation and dry chemistry were statistically significant in the MM group and control group, as non MM group and control group ,while they were not statistically significant in the groups of MM and non MM. Conclusion The total bilirubin concentration was affected by diazotization and vanadate oxidation in patients with paraprotein,while paraprotein in patients with MM and non MM patients has different effects on the determination of bilirubin by diazo method.

Objective To investigate the influence of male reproductive system infection on semen quality and male infertility. Methods Three hundred and twenty-eight male infertile patients with previously or currently reproductive system infection were selected,Among them 140 patients were infected with Ureaplasma urealyticum 93 with Chlamydia trachomatis,41 with cytomegalovirus,and 54 with mumps virus. The control group was 230 healthy men with reproductive history,perform semen quality analysis on the semen samples of the subjects above. Results Compared with the control group,the sperm density and living rate of the infection group were significantly decreased （P<0.05）,sperm malformation rate was significantly higher（P<0.05）,and the semen volume,semen liquefaction time and pH value and other parameters were not statistically significant difference（P>0.05）. Conclusion Male reproductive system infection can lead to a decline in semen quality,affecting male fertility.

Objective To detect immunoglobulins and C3 and C4 in children with hand-foot-mouth disease so as to evaluate the immune status of the patients. Methods One hundred and two cases of children with hand-foot-mouth disease,including 47 cases of mild manifestations 55 cases of severe manifestations and 30 cases of healthy children Serum IgA,IgG,IgM,C3,and C4 levels were examined. Results A statistical significance （P<0.05） was noted among the three groups of children,and the child patients presented high levels of IgM and IgG antibodies compared with the healthy control. IgA,C3,and C4 levels was lower than those of healthy children. Conclusion The immune status of children with hand-foot-mouth disease produced a remarkable immunity against the virus infection,which may help for the early diagnosis and clinical managements.

Objective To approach the reasons and characteristics of question blood group among blood donors in the area,provide basis for accurate typing of blood and clinical blood transfusion safety. Methods The blood group examination of RBC blood group was proceed in healthy donors in our center from January 2008 to December 2015. All the unconformity of positive and passive defined ones and the agglutination of negative O cell for comparison were defined as question blood group. The question blood groups were sent to blood typing reference center for further identification. Its causes and characteristics were analyzed. Results Among 285 282 blood samples,375 cases of question blood groups were screened out with the positive rate of 0.139%. 168 cases were men with the positive rate of 0.110%; 228 cases were women with the rate of 0.172%. The positive rate of women was higher than that of men with a statistical significance. For the causes of question blood group,316 cases of specific antibody were detected,accounting for 79.8%,28 cases were cold agglutinin,account for 7.1%,21 cases were nonspecific antibody,accounting for 5.3%. Anti-M（24.4%）,cold antibody （21.9%）and ABO isoforms（19.3%） were common specific antibody of causing the question blood group. The AχB（27.9%） and A₂B （13.1%） isoforms of AB typing of blood and B3 isoforms（11.5%） of B typing of blood were common types of ABO isoforms of causing the question blood group. Conclusion Certain detection rate of question blood group exists among blood donors. Many reasons could cause the occurrence of question blood group. Accurate blood group examination and reason analyses of question blood group could provide an important basis for the safety of clinical blood transfusion.

Objective Irregular blood group antibodies were detected in patients to prevent the occurrence of allogeneic delayed hemolytic transfusion reaction （DHTR） and transfusion safety. Methods Classical anti-human globulin test was taken to detect the specific antibodies in the serum of patients. Results The serum titers of IgM and IgG of anti-E were 1∶32 and 1∶128,respectively. Conclusion Rh antigen （CcEe） typing is necessary in the patients who have primary blood transfusion of red blood cells. Screening of irregular antibodies before transfusion is especially important to reduce the reverse reaction of hemolytic transfusion and ensure the clinical blood transfusion safety.

Objective To research the method of freeze-dried cryoprecipitate and evaluate its effect. Methods Forty units of fresh whole blood （200ml） were collected from volunteer donors. Two bags of cryoprecipitate with same capacity were prepared from each unit of whole blood by conventional method,one bag was stored at-20℃ as frozen cryoprecipitate,and the other was prepared into freeze-dried cryoprecipitate after adding Compound Amino Acid Injection by lyophilizer and stored at 4℃. Two sets of cryoprecipitates from 20 donors were taken out and analyzed for factor Ⅷ,fibrinogen,and pH after 24 h,and the other cryoprecipitates were evaluated after 12 months. Results There are no significant differences of factor Ⅷ,fibrinogen,and pH between frozen and freeze-dried cryoprecipitate after 24 hours,and 12 months storage,respectively （P>0.05）. Conclusion The freeze-dried cryoprecipitate stored at 4℃ after 12 months remain normal factor Ⅷ and fibrinogen contents and can be used as blood reserve for wartime.

Objective To evaluate antineoplastic activity of granulocytes in blood donors screened by two different methods. Methods The anti-tumor genes were detected in 681 cases within 72 hours. The in vitro cytotoxicity assay of granulocytes and the anti-tumor gene test were carried out within 24 and 72 hours,respectively. 811 cases were detected. The results of immunoprecipitation were compared with those of 189 blood donors who had been tested for anti-tumor gene. The results were compared in 16 patients （excellent,good,moderate and low in 4 cases,respectively）. Blood donors were followed for 1 month and 3 months Of the gene detection and granulocyte in vitro killing test. Results The proportion of excellent blood donation was 8.4% and 11.0% was good. The excellent blood donation rate of granulocyte in vitro test was 19.9%,and the score of genetic testing was " Excellent "and" good "and 19.4% similar gene score and granulocyte in vitro killing test score roughly in line. Conclusion The result of genetic testing is stable,and the result is constant,which may be related to genetic information. The disadvantage is that it can not truly reflect the current anti-cancer activity of blood donors,and the cost of screening is high; the results of granulocyte in vitro killing test are unstable; The change of anticancer activity of the individual at different time may be related to the mood,physical condition and seasonal variation of blood donors. The advantage is that the screening cost is low and it is easy to be applied and popularized.

【Abstract】 Objective To ensure the safety of clinical blood transfusion,Comparison of known ELISA Detection,ALT negative combined with NAT detection of reactive specimens and the corresponding CLIA method to detect HBV were analyzed. The correlation of the three detection methods was analysed. Methods 17 508 cases of blood donors （excluding ones of the same positive in ELISA,ALT positive） were detected by NAT and CLIA. Results Nucleic acid was detected in 9 cases of DNA HBV reactive specimens,RNA HCV reactive specimens of 0 cases and 0 cases of RNA HIV reactive specimens. 1 case in ELISA method was "negative value" High specimens. In CLIA method HBV "two pairs of half" detection,7 cases of HBcAb reactivity,2 cases no response. Conclusion First,blood donors detect methods in NAT and ELISA method was complementary,NAT detection can make up the "window period" of ELISA method. Second,NAT and CLIA test results were consistent; Third,OBI may be the main types of HBV DNA NAT detected blood donors in Handan area. Fourth,to reduce blood transfusion infection risk,the "negative value" high specimen in ELISA,which affected the quality of blood,should not be used,

Objective To evaluate hospitals'satisfac;tion with blood transfusion servicein order to provide a basis forimproving the quality of service. MethodThe hospitals' satisfaction with blood supply,blood station service and bloodquality in medical administration departments blood transfusion departments within 50 general hospitalswere studied orrespondance. There are 20 the tertiary hospitals ,20 the secondary hospitals and 10 the private hospitals. ResultTheir satisfactions with blood department staff service attitude、blood box temperature、timely delivery of blood service were 90%,76% and 68%respectively;The satisfactions of blood quality,quality problem feedback and blood supply were 88%,72% and 86% respectively. The service demand of clinical hospital to blood center is reflected in the guidance and training of transfusion technology. Conclusion The results of hospital satisfaction and service needs should be used as an important index to measure the blood quality and service quality.Ensuring continuous improvement of quality management and the safety and effectiveness of clinical blood transfusion.