Abstract: Objective To understand acute upper respiratory trace infections （acute upper respiratory tract infection,AURTI） people hospitalized children with partial pulmonary virus（ human metapneumovirus,hMPV） infection status and the prevalence of hMPV advantage subtypes in Shenzhen area. Methods From June 2015 to June 2016 in shenzhen longhua district people's hospital and Hutian people's hospital hospitalized Children with AURTI nasopharyngeal drain or swallow test specimens 1 720,at the same time respiratory specimens from 60 healthy children of the same period were collected,Qiagen One - step RT-PCR method for screening of hMPV in the sample was employed,and amplification products randomly selected from 6 cases of hMPV positive specimens were sequenced by CEQ8800 sequencer through forward and reverse primers,BLAST software was used to sequence alignment analysis for sequencing of gene sequences with GenBank representative strains . Results Nasopharyngeal hMPV positive specimens of 362 cases were detected from 1 720 cases of hospitalized children with AURTI drains content or swallow test specimen,positive rate was 21.05% （362/1 720）,the boy's positive rate was 10.06% （173/1 720）,the girl's positive rate was 10.99% （189/1 720）,there was no statistically significant difference between male and female children with positive rate （χ²=0.917,P>0.05）,and healthy children hMPV were all negative; <1 year old group AURTI hospitalized with an hMPV infection rate was 31.46%,significantly higher than the other group,and the difference was statistically significant （χ²=6.105~11.973,P<0.05~0.01）;1~3 month AURTI hospitalized children with hMPV infection positive rate was 49.74%,significantly higher than the other month,difference was statistically significant between （χ²=7.927~15.038,P<0.05~0.01）; The virus nucleotide of the six hMPV positive strains was 91.0%~100.0%. 6 strains of hMPV positive strains belong to the A2b and B1 and B2 subtype respectively. Conclusionh MPV is one of the main pathogens of infection in children with AURTI in the hospital in Shenzhen area,occurs in winter and spring seasons and children under the age of 1,A and B are two prevalent genotypes.

Key words: Acute upper respiratory tract infection, Children, Human pneumovirus, Status, Genetic analysis