Abstract: Objective To understand the immune suppression method test the levels of serum creatine kinase MB isoenzyme （CK - MB） in shenzhen area in 5 months ~ 13 years aged healthy children,and provide the Results1062 cases of healthy children CK - MB storage anomaly detection rate was 66.20% （703/1062）,including reference range of analysis with the methodology,the difference of the CK-MB for the region to establish healthy children provides some references for the reference range.Methods Collected March to June 2016 medical care of hospital,1 062 cases of healthy children,according to the age is divided into five months to 3 years old preschool group,4~6 years old children and 7~13 years,primary competitions and other three groups,and selected the same healthy adults 390 cases,the immune suppression method is adopted to detect the contents of serum CK-MB,compared with methodology provides the reference range,content is beyond the scope of the specimen at the same time for troponin I （cTnI）,myoglobin （MYO） and electrocardiogram examination,and the results were analyzed.Results Preschool group,preschool and primary sections CK-MB content abnormal rate was 81.02% （239/295）,66.23% （251/379） and 54.90% （213/388）,differences between groups of CK-MB content abnormal rate was statistically significant （χ²= 3.71~15.95,P<0.01~0.05）,and 390 cases of healthy adults in serum CK - MB content < 24 U/L; Specimens of 703 cases of abnormal CK - MB content of cTnI levels are < 0.034 ng/ml,3 cases of specimens MYO results increased slightly>61.5 ng/ml,but then on the electrocardiogram trace examination,abnormality was not found; Preschool group,infants and children primary competitions in serum CK-MB content were 41.52±19.38 U/L,35.09±14.37 U/L and 28.15±7.86 U/L,between different age children in the serum CK-MB content difference was statistically significant （t=2.596~2.596,P<0.05）; Healthy children in serum CK - MB content was 34.97±10.64 U/L,healthy adults in serum CK - MB content was 16.05±7.13 U/L,between the two results was statistically significant difference （t=13.927,P<0.01）.Conclusion Detection of immune suppression in shenzhen area in 5 months ~ 13 years healthy children serum CK-MB in the abnormal rate is very high,no direct reference methodology to provide clinical reference range,therefore,regional health children CK-MB in time the normal reference range of immune suppression method should be established,in order to better serve the clinical,lest cause misdiagnosis and unnecessary confirmed project inspection.

Key words: Immune suppression method, Children, Creatine muscle enzymes MB isoenzyme