Abstract: Objective To observe the effects of gender，age，peripheral blood WBC and MNC count on the collection of peripheral blood hematopoietic stem cells in children. Methods From January 2016 to January 2018，28 cases of peripheral blood hematopoietic stem cells collected in the hospital，ranging in age from 2 to 10 years and the median age was 10. Among 31 collections，7 autologous cases were given chemotherapy combined with granulocyte colony stimulating factor （GCSF） for mobilization，and 24 allogenic cases received GCSF mobilization，and 4-5 days later，peripheral blood hematopoietic stem cells were collected. The mean single circulating blood volume was 7 593±2 248 mL and the single product volume was 126 ±42 mL. Stem cell products were subjected to blood routine counting and flow cytometry to determine the number of CD34+ cells. Results Achievements of monocytes （MNCs） were uncorrelated to gender and age （P=0.44; P=0.81） in single collection but associated with counts of peripheral WBCs and MNCs （P=0.03; P=0.01）. Moreover，CD34+cell counts were found to be not associated with gender，age and peripheral WBCs counts （P=0.15; P=0.79; P=0.66），but with pre-harvest counts of MNCs （P=0.02）. Twenty four patients had MNCs>3.75×109/L before collection. The number of MNCs in single-collected stem cell products was 9.88±3.16×108 / kg and the number of CD34+cells was 7.51±4.74×106/Kg. Conclusion The number of MNCs in peripheral blood is an important factor affecting the collection efficiency of peripheral blood hematopoietic stem cells in pediatrics. The number of peripheral blood MNCs>3.75×109/L after mobilization would benefit for evaluation of the effect of hematopoietic stem cell mobilization.

Key words: MNC , Children , Peripheral blood, Hematopoietic stem cell collection