Abstract: Objective To explore if the leukemia cells count affect the K⁺ （potassium） and Glu （glucose） detection in PB （peripheral blood）. Methods According to the clinical diagnose and the leukemia cells count,group the leukemia PB samples. K⁺ and Glucose concentration were detected. Leukemia cells were isolated from peripheral blood,labeled by PI and Annexin-V and detected by flow cytometry. Results Individual case indicated that pseudo-hyperkalemia may be present when WBC count is high. In PB samples no matter collected from acute or chronic leukemia patients,compared with control group （WBC<20×10⁹/L）,the K⁺ concentration increased significantly in WBC （20~50,50~100 and >100）×10⁹/L groups,the Glucose concentration decreased significantly in WBC （50~100 and >100） ×10⁹/L groups. Necrosis rate of leukemia cells increased significantly as in vitro time increased. K⁺ and glucose concentration change was not significant in 6 hours after separate serum from blood cells. Conclusion leukemia cells count affects K⁺ and Glucose concentration in PB,cell necrosis is part of the important reasons. Separation serum is one of effective way to avoid pseudo-hyperkalemia and pseudo-hypoglycemia in PB samples from leukemia patients.

Key words: leukemia cell, Blood potassium, Blood glucose, Peripheral blood