Abstract: Objective To learn the effect of paraprotein in bilirubin measured by diazo method, vanadate oxidation and dry chemistry through bilirubin concentration basing on in the patients with paraprotein. Methods The serum of 247 cases of patients with paraprotein positive from Anqing Municipal Hospital from August 2015 to June 2017 was collected and divided into treatment groups: multiple myeloma (MM) group and non MM group. The serum of 50 case of patients with negative paraprotein was collected as the control group.Each sample was analyzed by dry chemical method, diazonium method, and vanadate oxidation method.. Analyse the test results of the determination of bilirubin in ways of diazo and vanadate compared with dry chemical. Results The difference of the bilirubin concentration by the means of diazonium and vanadate oxidation were statistically significant in the group of MM and non MM,while the difference were not statistically significant in the control group.The D-value of diazo method and dry chemistry were statistically significant in the MM group and control group, as in the groups of MM and non MM,meanwhile they were statistically significant in the non MM group and control group.The D-value of vanadate oxidation and dry chemistry were statistically significant in the MM group and control group, as non MM group and control group ,while they were not statistically significant in the groups of MM and non MM. Conclusion The total bilirubin concentration was affected by diazotization and vanadate oxidation in patients with paraprotein,while paraprotein in patients with MM and non MM patients has different effects on the determination of bilirubin by diazo method.

Key words: Paraprotein, Bilirubin, Diazonium method, Vanadate oxidotion method, Dry chemical method, Influence