Abstract: Objective To retrospectively discuss and analyze the influencing factors of multiple transfusions on cross transfusion in the patients with extensive burns in order to formulate an appropriate detection and blood cross matching strategy. Methods One hundred and eleven patients with large area （more than 85% ） burn were collected in the surgery department. Among the patients who had received transfusion and anti-infection treatments,the cross matching incompatibility was seen on the primary and secondary sides. Therefor the red blood cells and alloantibodies of patients were detected with absorption and elution technique. Results Forty eight of 111 （43.2％） patients were found to have alloantibodies,26 of them （23.4％） had erythrocyte direct antihuman globulin antibodies,23 of them （20.7%） presented positive abnormal protein aggregation,and 14 of them（12.6%） showed positive antibodies against medicine used. Conclusions A long term use of antibiotics,frequent transfusions of red blood cell suspension and leukocyte poor red blood cells,albumin,frozen plasma,and cryoprecipitate will result in alloantibodies,drug antibodies,autoantibodies,and erythrocyte sensitization,constituting the factors influencing blood cross matching incompatibility.

Key words: Extensive burn, Multi-blood transfusion, Anti-infection, Treatment, Cross-matching incompatibility