Abstract: Objective To establish the treatment technique of therapeutic plasma exchange （TPE） by domestic blood separator （NGL-XCF3000） at bedside for the clinical application to treatments in 154 patients. Methods Medical records of 154 patients，who received TPE treatments in the hospital between 2010 and 2016，were collected and retrospectively analyzed. The TPE at bedside was established，and the clinical feasibility was studied. Results The overall efficacy rate of the treatment technique was 86.4%. The results of TPE clinical applications showed a total effective rate of 83.8% in 74 cases of Guillain-Barre syndrome. An improvement of dyskinesia was seen in the patients when the TPE was used combined with glucocorticoids （P<0. 05）. Similarly，the effective rates of 90 per cent and 86.7 per cent were achieved in 50 cases of myasthenia gravis and 30 cases of acute liver failure，respectively. The overall incidence of adverse effects was found to be 8.4% in 154 cases. Conclusion The domestic products are safe and effective for the therapeutic plasma exchange at bedside and worth using clinically.

Key words: Therapeutic plasma exchange, Treatment technique at bedside, Clinical application