Abstract: Objective To observe the accuracy and sensitivity of the 2 types of Automatic Blood Grouping Instruments in Direct Antiglobulin Test （DAT）,we compare the false-positive rate,false-negative rate as well as the performances of instruments between the Automation Blood Grouping Analyser System of ORTHO AutoVue Innova and the blood grouping system which were composed of Ai gel 300 and the antigen detection card of Jiangyin Libio. Methods 500 randomLy selected patient samples were detected for DAT by above two types of instruments. The results were verified by Intube method of DAT. Results The ORTHO AutoVue Innova got the false-positive rate of 8.98% without detecting any false-negative sample. Meanwhile,the blood grouping system composed of Ai gel 300 and the antigen detection card of Jiangyin Libio showed 30.6% of the false-negative rate of and 2.25% of the false-positive rate. There was significant difference in detection between these two systems（P<0.05）. Conclusion The results show that ORTHO AutoVue Innova has obvious better performances and more clinical practice value in DAT.

Key words: Blood grouping instruments, Direct antiglobulin test （DAT）