Abstract: ObjectiveTo analyze anti-D and genotype of the partial D phenotype in a pregnant women. MethodsThe RhDCcEe antigens were detected in saline by microcolumn gel card method. The serum anti-D and its titers were detected by routine serological method. The partial D phenotype was confirmed by detecting 10 RHD exons,zygosity and Depitops. ResultsThe pregnant women was found to have dCcee in saline,but DCcee in gel card. RHD gene was found to have deletion of exons 3-6 and the zygosity was D/d. Some of the red cell membrane D epitopes were negative. The results revealed a partial DVI-III phenotype with DVICe/dce genotype. The titer of anti-D was 1：32 in week 20 of pregnancy and 1：64 just before delivery. ConclusionSeveral pregnancies with partial DVI-III phenotype have been reported who presented positive anti-D reaction and hemolytic disease of the newborns. DVI-III is not rare in Chinese and misdiagnosis should be avoidedI in prenatal examination.

Key words: Rh, blood, group, D, antigen, Partial, D, Anti-D, Antibody