Abstract: Objective To study the effect of direct anti-human globulin test (DAT) on the clinical efficacy of transfusion patients. Methods Collect 60 clinically DAT-positive patients to be transfused as the study group, and select 60 DAT-negative patients to be transfused during the same period as the control group. At the same time, 60 DAT-positive specimens were tested for antibody typing and complement typing, antibody strength determination, Comparative analysis of the marked efficiency after blood transfusion was performed in two groups of patients and patients with different types and antibody intensities of DAT-positive antibodies. Results After transfusion, the effective rate of DAT-negative patients was 88.33%, and the effective rate was 63.34%, the effective rate of DAT-positive patients was 75.00%, and the effective rate was 26.67%, which was statistically significant（P<0.01）. The antibody types of 60 DAT-positive patients were mainly composed of multiple antibody groups（including IgG + C3d and IgG + C3d antibodies containing IgM, IgA antibodies and / or C3c complement）, accounts for 61.67%, IgG alone accounts for 20.00%, C3d accounts for 18.33%. The efficiency of C3d group after transfusion was higher than that of IgG group（P<0.05）, and the efficiency of IgG group was higher than that of multiple antibody group（P<0.05）. The comparison of different antibody strengths found that the significant efficiency after transfusion in the 3+ ~4+ group was significantly lower than that in the 1+ group, which was statistically significant（P<0.01）. Conclusion Positive anti-human globulin test is one of the important factors affecting the effect of blood transfusion treatment, and the treatment effect has a certain relationship with antibody type and antibody intensity. Therefore, DAT detection, antibody type and antibody intensity have important reference significance for the effect of transfusion treatment and prognosis of anemia patients.

Key words: Direct anti-human globulin test, Antibody type, Antibody intensity, Transfusion effect