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  • 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)

Responsible Institution:

Anhui Commission of Health

Sponsor:

The First Affiliated Hospital of University of Science and Technology of China (Anhui Provincial Hospital) Anhui Provincial Association of Transfusion

Editor-in-Chief:XU Ge-liang

Publication Frequency:Bimonthly

CSSN:

ISSN 1671-2587

CN 34-1239/R

Current Issue

2021, Vol.23, No.2 Date of publication:20 April 2021
Effects of Resveratrol on Platelet Oxidative Stress and Aging Index and Storage Time
JIA Yan-jun, ZHAO Xu-ying, WEI Zhen-zhen
JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE. 2021 (2):  137-141.  DOI: 10.3969/j.issn.1671-2587.2021.02.001
Abstract ( 534 )   PDF(1287KB) ( 903 )  
Objective To explore the effects of resveratrol on platelet oxidative stress and aging index levels and storage time. Methods Plasma rich in platelets was prepared from 6-week-old C57 black 6 mice. Each sample was equally divided into observation group and control group. The observation group was added with resveratrol, and dimethyl sulfoxide was used to calibrate it to 50 μmmol /L, the control group was added with the same amount of dimethyl sulfoxide. Stored in 1, 2, 3, 4, 5, 6, and 7 days for sampling, testing content including: mean platelet volume(MPV), platelet count, pH, glucose levels, reactive oxygen species(ROS)level, proportion of Senescence-associatedβ-galactosidase staining positive cells, platelet CD62P, membrane surface proteins associated protein Ⅴ and MitoSpy positive level. Results During the observation period, there was no significant difference in MPV, platelet count, pH, and glucose level between the two groups at each time point(P>0.05). The ROS level of the observation group at the 5th and 6th day of storage were significantly lower than control group(t=9.977,10.650, P=0.000, 0.000), the SA-β-gal activity intensity of the observation group at the 2 d, 3 d, 4 d, 5 d, 6 d, 7 d were significantly higher than control group(t=3.237, 4.004, 4.909, 3.875, 4.392, 3.359; P=0.013, 0.001, 0.000, 0.002, 0.000, 0.010). There was no statistical difference between the platelet spreading and aggregation levels observed at 1d(P>0.05), the spreading and aggregation levels of the observation group at 3 d and 5 d were significantly above(t=3.096, 3.396, P=0.015, 0.007). Observation group in the storage of 2 d, 3d, 4 d, 5 d, 6 d, 7 d days of platelet CD62P activity were significantly lower than the control group(t=3.175, 3.345, 3.851, 5.169, 4.358, 7.601, P=0.017, 0.010, 0.002, 0.000, 0.000, 0.000), the storage of 3 d, 4 d, 5 d, 6 d, 7 d days of membrane protein V activated platelets were significantly lower than the control group(t=13.480,13.260,14.870,3.223,8.716;P=0.000,0.000,0.000,0.015,0.000), the storage of 5 d,6 d, 7 d MitoSpy positive blood platelet were significantly higher than control group(t=4.279,7.702,10.270;P=0.000,0.000,0.000). Conclusion sResveratrol can significantly improve platelet oxidative stress, aging index,the spreading and aggregation and the storage time during storage, it has good application potential.
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Comparison of the Effect of Rehydration Solutions Prepared in Different Ingredients on Freeze-dried Platelet-rich Plasma Activation and Release of Growth Factors
LIN Fang, ZHOU Mou, SHAN Gui-qiu, et al
JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE. 2021 (2):  142-145.  DOI: 10.3969/j.issn.1671-2587.2021.02.002
Abstract ( 620 )   PDF(1008KB) ( 910 )  
Objective To evaluate the efficacy of rehydration solutions prepared in different ingredients on freeze-dried platelet-rich plasma(FD-PRP)activation and release of growth factors. Methods The experiments were divided into four groups: control group, platelet-poor plasma(PPP); group 1, saline solution; group 2, ultrapure water; group 3, phosphate-buffered saline(PBS)buffer according to the difference between rehydration solutions. After rehydrated FD-PRP with four rehydration solutions, the contents of CD63, P-Selectin, transforming growth factor-beta 1(TGF-β1), vascular endothelial growth factor(VEGF), platelet-derived growth factor-BB(PDGF-BB)and basic fibroblast growth factor(bFGF)were determined by ELISA. Results While P-Selectin and CD63 expression in saline group, ultrapure water group and PBS buffer group were slightly higher than those in PPP group, there was no significant difference. The levels of growth factors in other three rehydration solutions were lower than those in PPP group. There was no significant difference in other four growth factors levels between other three groups and PPP group, except for the statistically significant difference in insulin-like growth factor-I(IGF-I). Conclusion Normal saline is widely available and safe. As a rehydration solution, it had little impact on freeze-dried platelet activation. The levels of growth factors had no significant difference compared to PPP rehydration solution. It can be used as a routine rehydration solution for FD-PRP.
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Down-Regulation of S100A1 Negatively Regulates Wnt/β- catenin Signaling Activity and Inhibits Proliferation of Thyroid Cancer SW579 Cells
ZHENG Yan, ZHANG Qian, SUN Fei
JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE. 2021 (2):  146-152.  DOI: 10.3969/j.issn.1671-2587.2021.02.003
Abstract ( 486 )   PDF(2715KB) ( 1310 )  
Objective To explore the effect of S100A1 on the proliferation of human thyroid cancer SW579 cells and its mechanism. Methods The human thyroid cancer data sets GSE50901 and GSE138198 were selected, and R software was used to screen the differentially expressed genes in thyroid cancer tissues and normal thyroid tissues. The differentially expressed genes were verified with TGCA database. CCK-8 experiment was used to detect the effect of S100A1 knockdown or overexpression on the proliferation of human thyroid cancer SW579 cell. Western Blot was used to detect the effect of S100A1 on the WNT/β-catenin signaling pathway. Results A joint analysis of the data sets GSE50901 and GSE138198 showed that 97 genes were up-regulated in both data sets, and 46 genes were down-regulated. S100A1 gene is highly expressed in thyroid cancer. Knockdown of S100A1 could inhibit the proliferation of SW579 cells, and overexpression of S100A1 could promote the proliferation of SW579 cells. GSEA analysis found that WNT signal pathway was significantly enriched after knocking down S100A1 in SW579 cells, and the signal pathway activity was reduced. Western Blot results showed that the protein expression levels of β-catenin, c-myc and cyclin D1 in the siS100A1 group were significantly lower than those in the siNeg group, while the protein expression levels of β-catenin, c-myc and cyclin D1 in the S100A1 group were significant higher than those in the EGFP group. Conclusion S100A1 is highly expressed in thyroid cancer. Knockdown of S100A1 might inhibit the proliferation of thyroid cancer SW579 cells by inhibiting the activity of WNT/β-catenin signaling pathway.
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Investigation of CD36 Antigen Deficiency in Unpaid Blood Donors in Hefei
ZHAO Na-na, WANG Chao, JIN Hui-xin, et al
JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE. 2021 (2):  152-155.  DOI: 10.3969/j.issn.1671-2587.2021.02.004
Abstract ( 771 )   PDF(1342KB) ( 724 )  
Objective To investigate the frequency of CD36 antigen deficiency in blood donors in Hefei. Methods A total of 973 blood samples of healthy platelet donors were randomly collected in Hefei blood center from March 2020 to July 2020. Flow cytometry was used for CD36 antigen detection. Platelet-rich plasma was prepared for detection CD36 antigen on platelets, and the residual cells were treated with erythrocyte lysate for CD36 antigen on monocytes. Results Among 973 platelet donors,16 donors were found platelet CD36 antigen deficiency, of which 2 were type I CD36 deficiency,14 were type II CD36 deficiency with the frequency 0.21% and 1.44% respectively. Conclusion The blood donors population in Hefei has certain CD36 antigen deficiency.
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Analysis of the Changes and Influencing Factors of Blood Cells before and after the Collection of Mononuclear Cells in Patients with Non-Hodgkin's Lymphoma
CHI Hong-xu, JIANG Ying, YAO Huan, et al
JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE. 2021 (2):  156-162.  DOI: 10.3969/j.issn.1671-2587.2021.02.005
Abstract ( 644 )   PDF(2056KB) ( 765 )  
Objective To improve collecting efficiency , we investigated the changes of blood cells and influencing factors of collecting mononuclear cells from peripheral blood before CAR-T treatment. Methods MNC cells were collected from 23 Non Hodgkin's lymphoma patients and 22 healthy donors by using COM.TEC blood cell separator. Routine blood test was performed before and after MNC collection, including red blood cell (RBC), white blood cell(WBC), lymphocyte cell, mononuclear cell and platelet count. The changes of blood cells before and after collection were analyzed. At the same time, the age, height, weight, body mass index (BMI), collection time, white membrane pumping volume, single collection volume, circulation times, single circulation volume, total circulation volume and the final product mononuclear cell count(MNC)were analyzed by multiple linear regression. Results The blood cell count of healthy donors and non-Hodgkin's lymphoma patients decreased after MNC collection(all P<0.05); Multiple linear regression analysis showed that the pre-collection MNC, single collection volume and cycle times were closely related to the final product MNC (all P<0.05). However, age, height, weight, BMI, systemic circulation blood volume, and flow rate, had no significant correlation with the final product MNC (all P>0.05). Conclusion sIn order to ensure the health level of patients and healthy donors, we should make individual collection plan for different recipients. The volume of single collection, the number of cycles and the count of pre-collection monocytes cells are the main factors for the efficiency of MNC collection.
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Factors Influencing Blood Transfusion in Patients after Unrelated Umbilical Cord Blood Transplantation
WANG Hui-ru, LIU Hui-lan
JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE. 2021 (2):  162-166.  DOI: 10.3969/j.issn.1671-2587.2021.02.006
Abstract ( 516 )   PDF(1301KB) ( 893 )  
Objective To investigate the related factors associated with blood product and its dose in unrelated umbilical cord blood transplantation (UCBT) patients. Methods The blood transfusion data of 29 patients with hematologic diseases undergoing UCBT were reviewed. The patients were divided into two groups: low dose group, a total of red blood cell (RBC) transfusions and platelet transfusions <18 U; high dose group, a total of RBC transfusions and platelet transfusions ≥18 U. The relationship between pre-transplant clinical data, implantation after transplantation, the incidence of acute graft-versus-host disease(aGVHD), one-year survival rate and blood transfusion were analyzed. Results The average volume of transfused RBCs in 29 patients within 60 days after transplantation was (10.97±11.80) U, and the average volume of transfused platelets was (7.12±5.06) U. There was a significant positive correlation between platelet and RBC transfusions. There was no significant difference in clinical data between the two groups before transplantation. There was a positive correlation between time of neutrophils implantation and platelet transfusions volume (P<0.05). RBC and platelet transfusions in patients with grade Ⅱ~Ⅳ aGVHD after transplantation were significantly higher than those in patients with grade 0 ~Ⅰ acute GVHD. The one-year survival rate in low dose group was significantly higher than that in high dose group (P <0.05). Conclusion The volume of RBC and platelet transfusions in UCBT patients within 60 days after transplantation was significantly correlated with donor implantation, aGVHD and survival after transplantation.
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The Clinical Effect of Plasma Exchange in ABO Incompatible Relatives in vivo Kidney Transplantation
ZHANG Yuan-yuan, YING Mei-ai, PAN Jian, et al
JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE. 2021 (2):  167-172.  DOI: 10.3969/j.issn.1671-2587.2021.02.007
Abstract ( 685 )   PDF(1095KB) ( 725 )  
Objective To observe the clinical effect of plasma exchange in removing blood group antibody in ABO incompatible relatives kidney transplantation. Methods The clinical characteristics of 54 cases of ABO non-identical relatives kidney transplantation in our hospital from July 2017 to January 2019 were analyzed retrospectively, and the changes of blood group antibody titer after plasma exchange treatment were analyzed. Results The titers of blood group antibodies (including IgG and IgM) were successfully reduced to the preoperative target titer in 2/54 patients, and renal transplantation was successfully carried out in 1/52 patients; One patient had the rebound titer of blood group antibody after operation, and the transplanted kidney failed. Two patients did not reduce the titer of blood group antibody to the target level. They gave up the kidney transplantation and continued the hemodialysis replacement treatment. There were 49 mild adverse events in 128 plasma exchange procedures. Conclusion Plasma exchange is an effective method to reduce the blood group antibody (including IgG and IgM) titer of recipients, which can be used in combination with other immunosuppressants to shorten the pre-operation time of patients with high blood group antibody titer.
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Risk Factors for Adverse Reactions and Strategies for Nursing in Perioperative Autologous Blood Donation
TIAN Jing, CHEN Feng, LIU Xiao-min, et al
JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE. 2021 (2):  173-177.  DOI: 10.3969/j.issn.1671-2587.2021.02.008
Abstract ( 544 )   PDF(1905KB) ( 610 )  
Objective To discuss risk factors for adverse reactions and strategies for nursing during perioperative autologous blood donation. Methods A total of 2 036 patients with pre-operative autologous blood donation (PABD) in the first medical center of Chinese PLA General Hospital between January 2017 and May 2019 were selected. We collected general data of patients before donation, recorded the incidence, types and factors associated with adverse events (AEs) during PABD procedure, and worked out strategies for nursing. Results Among the 2 036 patients included in the study, 31 (1.52%) experienced adverse reactions, of which vertigo (64.52%), weakness and sweating (41.94%), pallor (38.71%), nausea (25.81%) were the most common. Age, with underlying medical conditions, stressful blood draw and the number of punctures were the main risk factors associated with AEs during PABD. Conclusion In view of risk factors associated with adverse reactions, the corresponding PABD management and strategies for nursing can effectively reduce the incidence of adverse reactions to protect patients to the greatest extent, and ultimately, ensure the operation smoothly.
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The Change of Laboratory Indexes after Exchange Transfusions for Neonates
LIU Qian, WANG Xiao-huan, GUO Kai, et al
JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE. 2021 (2):  177-180.  DOI: 10.3969/j.issn.1671-2587.2021.02.009
Abstract ( 494 )   PDF(975KB) ( 574 )  
Objective To explore the effect of exchange transfusions (ETs) on the internal environment of newborns, and to provide guidelines for safe procedure. Methods Fourty neonates were included with haemolytic disease of the foetus and newborn(HDFN)from January 2016 to December 2018. The change of blood routine examination, biochemistry and coagulation indicators were evaluated before and after ETs. Results After ETs, total bilirubin, direct bilirubin and indirect bilirubin decreased from 390.4±17.98 μmol/L, 18.41±1.038 μmol/L and 370.4±18.16 μmol/L to 271.6±12.93 μmol/L, 8.08±0.97 μmol/L and 255.5±13.4 μmol/L respectively(P<0.001); There was no significant difference in prothrombin time(PT)and activated partial thromboplastin time(APTT)(P>0.05); Red blood cells count, hemoglobin and blood glucose increased from(3.1±0.13)×1012/L, 114.8±4.66 g/L, 3.92±0.19 mmol/L to(4.58±0.11)×1012/L, 137.3±3.18 g/L and 8.35±0.59 mmol/L respectively(P<0.001). Conclusion ETs can significantly decline the serum bilirubin level and improve clinical symptoms, but it showed some influence on some biochemical, blood routine and coagulation indexes. During and after the procedure, we should pay attention to these indexes for the safety of ETs.
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Retrospective Analysis of Rh (D,C, c, E, e) Antigens Compatibility between Blood Recipients and Donors
WANG Lei, WU Xue-zhong
JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE. 2021 (2):  181-185.  DOI: 10.3969/j.issn.1671-2587.2021.02.010
Abstract ( 910 )   PDF(1821KB) ( 1045 )  
Objective To explore the distribution of Rh(D, C, c, E, e)antigens and phenotypes of blood recipients and their donors in our hospital, and to analyze the compatibility of Rh antigens between recipients and donors as well as its clinical significance. Methods Cross-matching matched blood samples of 418 recipients and 710 donors were collected for Rh (D,C, c, E, e)antigens detection, and the distribution differences of antigens and phenotypes in recipients and donors as well as the antigen compatibility were compared and analyzed. Results There was no significant difference in Rh(D, C, c, E, e) antigens distribution between recipients and donors. The proportion of CcDEe and CCDee phenotype distribution was different between the recipients and the donors(P<0.05). The percentage of simultaneous incompatibility of C and e antigens between blood recipients and donors was the highest(50.00%)among Rh(D, C, c, E, e)antigen-incompatible transfusion. The recipients with ccDEE, ccDEe and CcDEE phenotypes had a higher probability of Rh antigen-incompatible transfusion. The recipients of transfusion from multiple donors had the highest probability of Rh antigen-incompatible transfusion(P<0.05). Conclusion There is a certain proportion of Rh antigen-incompatible transfusion in clinical transfusion. Detection of Rh(D, C, c, E, e)antigens before cross-matching and adoption of compatible transfusion can effectively improve the safety of clinical transfusion.
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The Clinical Use of Blood in Exchange Transfusion for Severe Neonatal Hyperbilirubinemia
GAN Shan, DUAN Ling, HU Hong-bing
JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE. 2021 (2):  185-188.  DOI: 10.3969/j.issn.1671-2587.2021.02.011
Abstract ( 539 )   PDF(1059KB) ( 803 )  
Objective By investigating the clinical use of blood for single disease, statistics and analysis of blood usage of neonates with hyperbilirubinemia receiving exchange transfusion therapy in our hospital were conducted to provide valuable evidence for rational blood storage in blood transfusion department, planned blood supply at blood station and rational clinical use of blood in clinic. Methods The clinical data of 389 neonates with hyperbilirubinemia receiving exchange transfusion therapy in our hospital between January 2016 and December 2019 were retrospectively analyzed. Data of patient's general condition were provided and the volumes of blood used for the exchange transfusion therapy were analyzed. Results Among the 389 cases of exchange transfusion, 212 cases were males, 177 cases were females. There was no statistically significant gender difference (P>0.05). One hundred and fifty five cases were blood type A, 128 cases were blood type B, 80 cases were blood type O, and 26 cases were blood type AB. There was significant difference between blood types (P<0.001). Among patients with hyperbilirubinemia, blood type O had the least volumes of blood for exchange, and there was no significant difference between blood type A and blood type B (P>0.05). Type O packed red blood cells and type AB fresh plasma were the most choice for exchange transfusion therapy. The amount of blood type O packed red blood cells was 1 060U, accounting for 86.14%. The amount of blood type AB plasma was 49 050 mL, accounting for 75.81%. The average transfusion used in each case was 3.13 units of packed red blood cells and 165 mL of fresh frozen plasma. Conclusion Through the analysis of exchange transfusion in severe neonatal hyperbilirubinemia, we summarized the experience of blood use for exchange transfusion, which will help blood transfusion department to develop a blood storage plan, provide evidence for optimizing transfusion practice. While effective treatment, Rational use of blood and saving blood have been emphasized.
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Clinical Significance of Direct Anti-human Globulin Test in Patients' Blood Transfusion Effect
DUAN Bing-zheng, WANG Shu-lian, LIAN Jun-hui, et al
JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE. 2021 (2):  189-191.  DOI: 10.3969/j.issn.1671-2587.2021.02.012
Abstract ( 993 )   PDF(961KB) ( 1187 )  
Objective To study the effect of direct anti-human globulin test (DAT) on the clinical efficacy of transfusion patients. Methods Collect 60 clinically DAT-positive patients to be transfused as the study group, and select 60 DAT-negative patients to be transfused during the same period as the control group. At the same time, 60 DAT-positive specimens were tested for antibody typing and complement typing, antibody strength determination, Comparative analysis of the marked efficiency after blood transfusion was performed in two groups of patients and patients with different types and antibody intensities of DAT-positive antibodies. Results After transfusion, the effective rate of DAT-negative patients was 88.33%, and the effective rate was 63.34%, the effective rate of DAT-positive patients was 75.00%, and the effective rate was 26.67%, which was statistically significant(P<0.01). The antibody types of 60 DAT-positive patients were mainly composed of multiple antibody groups(including IgG + C3d and IgG + C3d antibodies containing IgM, IgA antibodies and / or C3c complement), accounts for 61.67%, IgG alone accounts for 20.00%, C3d accounts for 18.33%. The efficiency of C3d group after transfusion was higher than that of IgG group(P<0.05), and the efficiency of IgG group was higher than that of multiple antibody group(P<0.05). The comparison of different antibody strengths found that the significant efficiency after transfusion in the 3+ ~4+ group was significantly lower than that in the 1+ group, which was statistically significant(P<0.01). Conclusion Positive anti-human globulin test is one of the important factors affecting the effect of blood transfusion treatment, and the treatment effect has a certain relationship with antibody type and antibody intensity. Therefore, DAT detection, antibody type and antibody intensity have important reference significance for the effect of transfusion treatment and prognosis of anemia patients.
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An Evaluation Model of Clinical Blood Transfusion Records Based on Cov-AHP-fuzzy Comprehensive Evaluation Method
YANG Chun-chen, RONG Rui-ming, LI Yang, et al
JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE. 2021 (2):  192-196.  DOI: 10.3969/j.issn.1671-2587.2021.02.013
Abstract ( 783 )   PDF(1047KB) ( 1166 )  
Objective To construct an evaluation model of clinical blood transfusion records based on Cov-AHP-fuzzy comprehensive evaluation method. Methods An index system for the quality evaluation of clinical blood transfusion records was established by the professional scoring method. The weight of each index was determined by covariance-analytic hierarchy process (Cov-AHP). Finally, 100 clinical blood transfusion records were collected and evaluated to verify the rationality of the model. Results The evaluation index system was established according to four dimensions, including blood transfusion application process, blood transfusion document, blood transfusion evaluation and blood transfusion nursing. The weight of each index was determined. The evaluation result of the 100 records was consistant with the normal distribution. Conclusion This evaluation model can more scientifically and rationally evaluate the clinical blood transfusion records, and strengthen doctors' attention to clinical blood transfusion. In addition, the model can also supervise the clinical blood transfusion chain, improve haemovigilance system, and effectively reduce the occurrence of transfusion adverse events.
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Application of Quality Control Circle Activity in Reducing Emergency Transfusion Waiting Time of Severe Postpartum Hemorrhage Patients during Obstetric Surgery
GUO Gan-ping, JIANG Bi, WANG Xiang-dong, et al
JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE. 2021 (2):  197-201.  DOI: 10.3969/j.issn.1671-2587.2021.02.014
Abstract ( 525 )   PDF(1316KB) ( 704 )  
Objective To discuss the effect of quality control circle activity in emergency transfusion waiting time of severe postpartum hemorrhage patients during obstetric surgery. Methods First, emergency transfusion waiting time of postpartum hemorrhage patients (amount of bleeding≥1 000 mL) from January 2018 to March 2019 was reviewed. Then, according to the ten steps of quality control circle activities, the links of emergency transfusion during obstetric surgery were improved. At last, the difference of the emergency transfusion waiting time of severe postpartum hemorrhage patients and the satisfaction of emergency transfusion in operating room between before the operation of quality control circle activity and after it was compared. Results The emergency transfusion waiting time of severe postpartum hemorrhage patients was 53±11 minutes and the satisfaction of operating staff with emergency transfusion was 70% before the quality control circle activity;The emergency transfusion waiting time of severe postpartum hemorrhage patients is 33±8 minutes(t=7.5,P<0.05) and the satisfaction of operating staff with emergency transfusion is 95% after the quality control circle activity. Conclusion The improvement of the link of emergency transfusion during obstetric surgery can reduce emergency transfusion waiting time of severe postpartum hemorrhage patients and improve the satisfaction of operating staff with emergency transfusion.
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Meta-analysis:Unexpected Antibody Positive Rate of Blood Donors in China
GUO Wei-jie, LIU Ze-ya, ZHANG Fan, et al
JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE. 2021 (2):  202-212.  DOI: 10.3969/j.issn.1671-2587.2021.02.015
Abstract ( 650 )   PDF(1420KB) ( 1196 )  
Objective Meta-analysis was performed to analysis positive rate and distribution characteristics of unexpected antibodies in Chinese blood donors to provide data for judging whether blood donors in China needs to carry out unexpected antibodies detection. Methods We searched PubMed,CNKI,WanFang Data,VIP,and CBM databases and entered specific search terms for relevant literature on unexpected antibodies in Chinese donors from inception to December 2019. According to inclusion and exclusion criteria,five researchers independently screened literature, extracted relevant data and assessed the methodological quality. The R3.6.2 software was used to perform meta-analysis. Results A total of 38 cross-sectional studies were included. Meta-analysis results showed that total antibody positive rate of donors in China was 0.20% [95%CI(0.15%,0.25%)]. The antibody positive rate of male donors was 0.13% [95%CI(0.10%,0.16%)], and that of female donors was 0.31% [95%CI(0.26%,0.37%)]. The antibody positive rate of female donors was higher than that of male donors. There was no statistically significant difference. Among the positive antibodies, the most abundant antibody isotype was IgM,accounting for 59.78%[95%CI(50.01%,69.54%)]. IgG antibody accounted for 31.33% [95%CI (23.44%, 39.22%)] of the total positive antibodies and IgG antibodies were more common in females. Based on the results of antibody identification, the positive rate of antibody in each blood group system was different: Rh system accounting for about 31.64%[95%CI(25.25%,38.03%)] (about 99.61% were IgG antibodies,mainly anti-D and anti-E),MNS system accounting for about 23.73%[95%CI(18.80%,28.65%)] (about 88.74% were IgG antibodies,mainly anti-M),Lewis system accounting for about 8.18%[95%CI(5.71%,10.66%)] (mainly IgG antibodies,mainly anti-Lea),P system accounting for about 4.54%[95%CI(2.58%,6.51%)] (mainly IgM antibody,mainly anti-P),other systems (including non-specific antibody and autoantibody) accounting for 22.78%[95%CI(14.09%,31,46%)] (mainly IgM antibody), Kell system accounting for about 0.05%[95%CI(0,0.46%)], Duffy system accounting for about 0.05%[95%CI(0,0.46%)] (mainly IgG antibody, mainly anti-Fya),Kidd system accounting for about 0.01%[95%CI(0,0.42%)] (all anti-Jka), and the Diego system accounting for about 0.01%[95%CI(0,0.4%)] (all anti-Dia). Antibody frequency ranking: anti-M>anti -D>anti -E>anti -Lea>anti -P>anti -Leb>anti -N>anti -cE>anti -C>anti -Fya>anti -Jka>anti -Dia. Conclusion It is necessary to bring the unexpected antibody screening test into routine project for blood donors. It will reduce experimental cost and benefit implementation of electronic cross-matching to develop special spectrum of blood donor screening cells aiming at most common unexpected antibodies present in blood donors.
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Epidemiological Characteristics of Recent HIV-1 Infection in Blood Donors in Fuzhou Area
LIN Shou, JIANG Wei-mei, ZHAN You-zhi, et al
JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE. 2021 (2):  212-215.  DOI: 10.3969/j.issn.1671-2587.2021.02.016
Abstract ( 673 )   PDF(970KB) ( 868 )  
Objective To understand the new HIV-1 infection status and distribution characteristics of unpaid donors in Fuzhou area, and provide evidence for decision-making for blood collection and supply institutions to formulate HIV prevention and control strategies. Methods Samples from unpaid donors in Fuzhou area that were confirmed to be positive for HIV-1 antibody, were tested by LAg-Avidity EIA and BED assays between 2011 and 2018, and the characteristics of the recently infected donors were analyzed. Results A total of 109 confirmed HIV positive samples were collected from 2011 to 2018, and 43 cases of new HIV infections were determined. The proportion of new infections accounted for 39.45%. Among all newly infected donors, 95.35% were males and 4.65% were females; 69.76% were under 30-year-olds, and 30.24% were over 30-year-olds; 62.79% were high school or secondary technical school or above, 37.21% were under high school, and 25.58% were college students. Conclusion sThe majority of new HIV-1 infected donors in Fuzhou area were younger males with higher education, and corresponding prevention and control strategies should be adopted for specific groups of people when pre-donation counselling and recruitment.
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Analysis on Curative Effect of VCD Regimen Combined with Ubenimex on Multiple Myeloma and Factors Influencing Prognosis
CHU Hai-liang, XING Ming-quan, GE Hong-feng
JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE. 2021 (2):  216-221.  DOI: 10.3969/j.issn.1671-2587.2021.02.017
Abstract ( 943 )   PDF(1194KB) ( 854 )  
Objective To explore clinical curative effect of VCD combined with ubenimex on multiple myeloma (MM) and to analyze influencing factors of prognosis. Methods The data of 96 MM patients who were admitted to the hospital from April 2015 to June 2019 were retrospectively collected. According to different treatment methods, they were divided into VCD group (47 cases) and combination group (49 cases). All patients were given VCD (velcade, cyclophosphamide, dexamethasone) regimen for chemotherapy. They were given continuous administration of ubenimex during chemotherapy in combination group. After 3 cycles of chemotherapy, curative effect was observed. The changes in biochemical indexes were measured. Adverse reactions and prognosis were statistically analyzed. Results The disease remission rate in combination group was significantly higher than that in VCD group (77.55% vs 57.45%) (P<0.05). Compared with those before treatment, plasmocyte proportion and serum M protein in bone marrow of both groups were significantly decreased, while hemoglobin (Hb) was significantly increased (P<0.05). The improvement effect in combination group was better than that in VCD group (P<0.05). There were adverse reactions such as constipation, lethargy, infectious fever and bone marrow suppression, all mainly on level Ⅰ-Ⅱ. There was no significant difference in incidence between the two groups (P>0.05). Of the 96 patients, 2-year overall survival (OS) rate and progression-free survival (PFS) rate were 76.04% and 61.46%, respectively. Univariate analysis showed that ISS staging, treatment regimen and curative effect were correlated with OS rate and PFS rate (P<0.05). COX multivariate analysis showed that ISS staging, treatment regimen and curative effect were all risk factors influencing OS rate (P<0.05). Only curative effect was the risk factor influencing PFS rate (P<0.05). Conclusion VCD regimen combined with ubenimex in treatment of MM can effectively improve clinical curative effect, adjust levels of biochemical indexes, and improve prognosis without increasing adverse reactions. In addition to treatment regimen, ISS staging and curative effect are also independent factors influencing prognosis.
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Association of Guillain-Barré Syndrome and Hepatitis B Virus Infection
YAN Song-xia, WANG Hai-yan, JING Zhong-cui, et al
JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE. 2021 (2):  222-226.  DOI: 10.3969/j.issn.1671-2587.2021.02.018
Abstract ( 804 )   PDF(936KB) ( 939 )  
Objective To investigate hepatitis B virus (HBV) infection in patients with Guillain-Barré syndrome (GBS), we discussed whether HBV infection was a short-term poor prognostic factor for GBS, based on the association between these two diseases. Methods This retrospective study included 115 patients hospitalized with GBS in the department of neurology, Affiliated Hospital of Qingdao University between January 2010 and December 2019. During the same period, 150 patients with non-GBS in the same department were selected as the control group.Five items of HBV were tested. We analyzed HBV infection and its correlation with the outcome of patients with GBS.We also collected data of the time from symptom onset to hospital admission, hospital inpatient days, history of pre-infection, limb muscle strength at the time of admission and discharge, blood biochemical indicators and outcomes etc..Statistical analysis of risk factors for affecting the prognosis of patients was performed. Results The positive rate of HBcAb in GBS patient group (52.2%) was higher than that in control group (37.3%). The proportion of patients with lower Medical Research Council (MRC) score and poor prognosis in positive HBcAb group was significantly higher than that in negative HBcAb group, with statistical differences (P<0.05). Logistic regression analysis in patients with poor prognosis showed that HBV infection, respiratory paralysis and lower MRC score were independent risk factors for affecting short-term prognosis of patients with GBS. Conclusion This study found that the positive rate of HBV infection in GBS patients was higher, and HBV infection was associated with the occurrence and short-term prognosis of GBS.
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Changes of Coagulation Function and Risk Factors in Early Pregnancy Patients with Missed Abortion
JIANG Tong-can, FANG Zhang-hua, REN Jun-jiao, et al
JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE. 2021 (2):  227-231.  DOI: 10.3969/j.issn.1671-2587.2021.02.019
Abstract ( 713 )   PDF(1842KB) ( 823 )  
Objective To investigate risk factors and the changes of coagulation function in early pregnancy patients with missed abortion. Methods The retrospective study was performed. A total of 349 patients diagnosed with missed abortion in early pregnancy in Maternal and Child Health Hospital Affiliated to Anhui Medical University between March 2018 and September 2018 were selected as observation group. 153 normal pregnancy women who had artificial abortion due to unplanned pregnancy at the same period were selected as control group. The characteristics of general information, blood routine and coagulation tests of the two groups were analyzed. Logistic regression analysis was used to identify risk factors. Results ①Age in observation group was older than that in control group(29.68±4.72 vs 27.64±5.38, P<0.001). The number of spontaneous abortions was also higher than that in control group(0.19±0.53 vs 0.06±0.29, P=0.001). ② The level of D-dimer was higher, and fibrinogen degradation product(FDP)and fibrinogen were lower in observation group compared to those in control group. In observation group, D-dimer level in recurrence group was higher than that in newly diagnosed group. Prothrombin time(PT), activated partial thromboplastin time (APTT) and thrombin time (TT) in observation group were higher than that in control group(P<0.01). There was no significant difference in platelet count between the two groups(P>0.05). ③Logistic regression analysis showed that age and history of spontaneous abortion were risk factors for missed abortion: Age≥25~30 years old (odds ratio(OR)5.690; 95% CI 3.184~10.168), age ≥30 years(OR 9.882; 95% CI 4.949~19.731), history of spontaneous abortion(OR4.994; 95% CI 1.881~13.257). Conclusion Although patients with missed abortion during early pregnancy showed hyperfibrinolysis, most patients had no obvious coagulation abnormality. Age and a history of spontaneous abortion were the risk factors for missed abortion. For women aged older than 25 years or with a history of spontaneous abortion in early pregnancy, coagulation tests might help reduce their incidence of missed abortion by appropriate interventions.
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Clinical Observation of Children with Immune Thrombocytopenia and the Study of the Effect of ANA/ANAs in the Disease
ZHENG Jing-xian, LI Xiao-hui, SUN Hong-qiang
JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE. 2021 (2):  231-235.  DOI: 10.3969/j.issn.1671-2587.2021.02.020
Abstract ( 773 )   PDF(1099KB) ( 1210 )  
Objective To explore the characteristics, the effect of different treatments, and the role of antinuclear antibody/antinuclear antibody spectrum (ANA/ANAs) of immune thrombocytopenia (ITP) in children. Methods We retrospectively analyzed the characteristics of 204 children with new-onset ITP in the First Affiliated Hospital of Harbin Medical University from February 2017 to January 2019. Among them, 135 cases who could be followed up were classified according to intravenous immunoglobulin(IVIg)or symptomatic treatment, ANA/ ANAs positive or negative. The basic characteristics and treatment response of different groups were compared. Results The initial platelet count of the patients treated with IVIg was lower than that in symptomatic treatment group, and the platelet elevation, complete response rate (CR), overall response (CR+R) rate and ANA/ANAs positive rate were higher than those in the symptomatic treatment group, but there was no difference in the chronic rate. The initial platelet count of patients with ANA/ANAs positive was lower than that of negative patients. On the 5th day after admission, the platelet elevation and CR rate of patients with ANA/ ANAs positive treated with IVIg were lower than those of negative patients. The time of platelet elevation to normal in ANA/ANAs positive patients was longer. There was no difference between the CR+R rate and chronic rate. Conclusion In the treatment of children ITP,IVIg can rapidly increase platelets, but there is no advantage in long-term response. The treatment response of ANA/ANAs positive patients is worse than that of negative patients, so it is necessary to take more active treatment in the early stage.
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