17例ABO血型鉴定中混合视野现象的分析及利用三代测序技术鉴定其等位基因

doi:10.3969/j.issn.1671-2587.2025.03.004

临床输血与检验 ›› 2025, Vol. 27 ›› Issue (3): 306-309.DOI: 10.3969/j.issn.1671-2587.2025.03.004

17例ABO血型鉴定中混合视野现象的分析及利用三代测序技术鉴定其等位基因

夏悦昕¹, 杨宜承¹, 李春祥¹, 周世航¹, 刘志远²

¹大连市血液中心;
²大连市妇女儿童医疗中心（集团）中心实验室, 辽宁大连 116000

收稿日期:2024-12-20 发布日期:2025-06-23
通讯作者: 周世航,主要从事免疫血液学研究,（E-mail）zshsail@163.com。共同通信作者刘志远,主要从事二代测序等相关研究,（E-mail）liubeiereagle@126.com。
作者简介:夏悦昕,主要从事免疫血液学研究,（E-mail）xiayuexin001@163.com。

Analysis of Mixed Vision in 17 Cases of ABO Blood Group Typing and Identification of Their Alleles Using PacBio SMRT Third-generation Sequencing Technology

XIA Yuexin¹, YANG Yicheng¹, LI Chunxiang¹, ZHOU Shihang¹, LIU zhiyuan²

¹Dalian Blood Center, Dalian 116000;
²Dalian Municipal Women and Children's Medical Center (Group), Dalian 116000

Received:2024-12-20 Published:2025-06-23

摘要/Abstract

摘要： 目的对17例在ABO血型鉴定中出现混合视野的血液标本进行单倍体基因型的测定以期准确鉴定ABO血型。方法利用免疫血清学试验鉴定ABO表型,采用PacBio单分子实时荧光（single molecule real-time, SMRT）测序技术检测单倍型。结果 17例标本免疫血清学试验均为亚型特征,三代基因测序技术结果显示其中亚型为9例,分别为：5例A1Bweak,2例cisAB,1例Bweak,1例B（A）;非亚型8例,分别为：6例A1B,1例A1,1例B。结论利用血清学试验及基因测序技术相结合的方法,通过测定其基因型,从而可以准确鉴定其血型,保障临床输血安全。

关键词: ABO基因分型, PacBio三代测序, 混合视野

Abstract: Objective Using PacBio third-generation sequencing technology to determine the haplotype genotypes of 17 blood samples exhibiting mixed vision in ABO blood group typing, with the aim of accurately identifying the ABO blood types. Methods The ABO phenotype was identified by immunoserological method. The ABO genotype was identified by PacBio Third-Generation sequencing technology. Results Seventeen specimens exhibited subtype characteristics in the immunological serology tests. The results of third-generation gene sequencing technology showed that nine of them were subtypes, including five cases of A1Bweak, two cases of cisAB, one case of Bweak, and one case of B(A). The remaining eight cases were non-subtypes, comprising six cases of A1B, one case of A1, and one case of B. Conclusion By combining serological tests with gene sequencing technology to determine the genotype, we can accurately identify blood types and improve the safety of clinical blood transfusions.

Key words: ABO genotype, PacBio third-generation sequencing technology, Mixed Vision

中图分类号:

R446.11Q78

夏悦昕, 杨宜承, 李春祥, 周世航, 刘志远. 17例ABO血型鉴定中混合视野现象的分析及利用三代测序技术鉴定其等位基因[J]. 临床输血与检验, 2025, 27(3): 306-309.

XIA Yuexin, YANG Yicheng, LI Chunxiang, ZHOU Shihang, LIU zhiyuan. Analysis of Mixed Vision in 17 Cases of ABO Blood Group Typing and Identification of Their Alleles Using PacBio SMRT Third-generation Sequencing Technology[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2025, 27(3): 306-309.

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17例ABO血型鉴定中混合视野现象的分析及利用三代测序技术鉴定其等位基因

Analysis of Mixed Vision in 17 Cases of ABO Blood Group Typing and Identification of Their Alleles Using PacBio SMRT Third-generation Sequencing Technology

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