RhD初筛阴性无偿献血者人群中D变异型漏检及频率研究*

doi:10.3969/j.issn.1671-2587.2025.03.015

临床输血与检验 ›› 2025, Vol. 27 ›› Issue (3): 373-377.DOI: 10.3969/j.issn.1671-2587.2025.03.015

RhD初筛阴性无偿献血者人群中D变异型漏检及频率研究^*

王菲, 武云香, 王华, 左江涛, 赵沛喆, 张欣, 李志诚, 张德梅

太原市血液中心输血技术研究室, 山西太原 030024

收稿日期:2024-09-01 发布日期:2025-06-23
通讯作者: 张德梅,主要从事临床输血与免疫血液学方面研究,（E-mail）13834532717@126.com。
作者简介:王菲,主要从事临床输血与免疫血液学方面研究,（E-mail）782903926@qq.com。
基金资助:
*本课题受太原市科技局太原市卫生健康委“六个一批”专项行动科研项目（No.Y2023013）、太原市医学重点学科、太原市医学科技创新平台资助

Miss Detection and Frequency of the D Variant in Initial RhD-negative Sample Screening

WANG Fei, WU Yunxiang, WANG Hua, ZUO Jiangtao, ZHAO Peizhe, ZHANG Xin, LI Zhicheng, ZHANG Demei

Taiyuan Blood Center, Blood Transfusion Research Laboratory, Taiyuan 030024

Received:2024-09-01 Published:2025-06-23

摘要/Abstract

摘要： 目的探讨太原地区RhD初筛阴性无偿献血者人群中D变异型的漏检率及频率。方法收集2013年1月—2021年12月太原地区6 287份RhD初筛阴性无偿献血者标本,使用试管抗球蛋白法或微柱凝集法进行RhD血型的确认检测,并对与以往检测结果不一致,以及采用不同厂家初筛试剂结果不一致的标本,共计17例,均进行RhD血型基因检测。通过对D变异型献血者人数、献血次数以及漏检次数的统计分析,得出RhD初筛阴性无偿献血者中D变异型的理论漏检率和理论频率。结果 2013年1月—2021年12月太原地区6 287份RhD初筛阴性献血者样本（包括重复献血者）中,D变异型检出率为5.36%（337/6 287）;对其中59位D变异型重复献血者统计分析显示,D变异型的理论漏检率为5.37%。纳入漏检的D变异型后,本组RhD初筛阴性无偿献血者人群中,D变异型的理论频率为7.68%。结论常规的血清学确认检测方法存在漏检D变异型的情况;本研究获得了在RhD初筛阴性无偿献血者人群中较为接近真实情况的D变异型的理论漏检率和理论频率。

关键词: RhD阴性, D变异型, D变异型漏检率, 重复献血者

Abstract: Objective To assess the miss detection rate (MDR) and frequency of the D variant among RhD negativeblood donors in Taiyuan. Methods From January 2013 to December 2021, 6 287 RhD negativeblood samples, initially screened, were collected. These samples were further confirmed using the tube antiglobulin test or microcolumn gel card method. RhD genotyping was done on the 17 specimens which showed discrepancies with previous testresults or the manufacturer's specifications for the primary screening reagent. By analyzing the number of D variant donors, blood donations and missed detections, the theoretical MDR and frequency of D variant were calculated in the RhD negativeblood samples from the primary screening. Results Between January 2013 and December 2021, the detection rate of D variant in the 6 287 RhD negative samples (including repeat donors) by preliminary screening was 5.36%（337/6 287）. Statistical analysis of 59 D variant blood donors who donated more than once revealed that theoretically, 5.37% of D variants were missed. Considering the MDR, the theoretical frequency of D variant in the RhD negative population from the preliminary screening was 7.68%. Conclusion Some D variant blood donors were missed in the conventional test. The theoretical MDR and frequency of D variant in the RhD negative samples by primary screening align more closely with the actual situation.

Key words: RhD negative, D variant, D variant miss detection rate, Repeat blood donor

中图分类号:

R457

王菲, 武云香, 王华, 左江涛, 赵沛喆, 张欣, 李志诚, 张德梅. RhD初筛阴性无偿献血者人群中D变异型漏检及频率研究^*[J]. 临床输血与检验, 2025, 27(3): 373-377.

WANG Fei, WU Yunxiang, WANG Hua, ZUO Jiangtao, ZHAO Peizhe, ZHANG Xin, LI Zhicheng, ZHANG Demei. Miss Detection and Frequency of the D Variant in Initial RhD-negative Sample Screening[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2025, 27(3): 373-377.

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RhD初筛阴性无偿献血者人群中D变异型漏检及频率研究^*

Miss Detection and Frequency of the D Variant in Initial RhD-negative Sample Screening

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